A WRKY Transcription Factor PmWRKY57 from Prunus mume Improves Cold Tolerance in Arabidopsis thaliana

Wang, Yiguang; Dong, Bin; Wang, Nannan; Zheng, Zifei; Yang, Liyuan; Zhong, Shiwei; Fang, Qiu; Zheng, Xiao; Zhao, Hongbo

doi:10.1007/s12033-022-00645-3

Cited by 18 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate the function of PbWRKY31 in plants, we first aligned the protein sequence of PbWRKY31 with other known WRKY31 protein sequences. As shown in Figure 8 A, PbWRKY31 consists of 606 amino acids, and the WRKY domain is highly conserved in pear, apple [ 22 ], grapevine [ 25 ], soybean [ 26 ], and Arabidopsis [ 27 ], particularly the 7-peptide WRKYGQK motif at the N-terminus [ 20 ], implying that they may have relatively conserved functions ( Figure 8 A).…”

Section: Resultsmentioning

confidence: 99%

“…On the contrary, OsWRKY31 has transactivation activity in yeast, indicating that sequences outside the WRKY domain may also be necessary for its activation activity [ 36 ]. Subcellular localization experiments found that PbWRKY31 located in and outside the nucleus, while WRKY31 from Arabidopsis , Populus × euramericana , rice, and soybean are all exclusively located in the nucleus [ 21 , 26 , 27 , 36 ], suggesting PbWRKY31 may also function outside the nucleus.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Time-Series Transcriptome Analysis Reveals the Molecular Mechanism of Ethylene Reducing Cold Sensitivity of Postharvest ‘Huangguan’ Pear

Wei

et al. 2023

IJMS

View full text Add to dashboard Cite

‘Huangguan’ pear (Pyrus bretschneideri Rehd) fruit is susceptible to cold, characterized by developing peel browning spots (PBS) during cold storage. Additionally, ethylene pretreatment reduces chilling injury (CI) and inhibits PBS occurrence, but the mechanism of CI remains unclear. Here, we deciphered the dynamic transcriptional changes during the PBS occurrence with and without ethylene pretreatment via time-series transcriptome. We found that ethylene suppressed the cold-signaling gene expression, thereby decreasing the cold sensitivity of the ‘Huangguan’ fruit. Moreover, the “Yellow” module closely correlated with PBS occurrence was identified via weighted gene co-expression network analysis (WGCNA), and this module was related to plant defense via Gene Ontology (GO) enrichment analysis. Local motif enrichment analysis suggested that the “Yellow” module genes were regulated by ERF and WRKY transcription factors. Functional studies demonstrated that PbWRKY31 has a conserved WRKY domain, lacks transactivation activity, and localizes in the nucleus. PbWRKY31-overexpressed Arabidopsis were hypersensitive to cold, with higher expression levels of cold signaling and defense genes, suggesting that PbWRKY31 participates in regulating plant cold sensitivity. Collectively, our findings provide a comprehensive transcriptional overview of PBS occurrence and elucidate the molecular mechanism by which ethylene reduces the cold sensitivity of ‘Huangguan’ fruit as well as the potential role of PbWRKY31 in this process.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Time-Series Transcriptome Analysis Reveals the Molecular Mechanism of Ethylene Reducing Cold Sensitivity of Postharvest ‘Huangguan’ Pear

Wei

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Enzymatic hybridization experiments have shown that ZmB12D interacts with ZmWRKY70 , regulating maize waterlogging resistance [ 46 ]. The PmWRKY70 gene was cloned from the Prunus mume cultivar “Guhong zhusha”, and the overexpression of PmWRKY57 enhanced the cold stress resistance of Arabidopsis thaliana plants [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Evolution of the WRKY Family in Angiosperms and Functional Diversity under Environmental Stress

Wu,

Yang,

et al. 2024

IJMS

View full text Add to dashboard Cite

The transcription factor is an essential factor for regulating the responses of plants to external stimuli. The WRKY protein is a superfamily of plant transcription factors involved in response to various stresses (e.g., cold, heat, salt, drought, ions, pathogens, and insects). During angiosperm evolution, the number and function of WRKY transcription factors constantly change. After suffering from long-term environmental battering, plants of different evolutionary statuses ultimately retained different numbers of WRKY family members. The WRKY family of proteins is generally divided into three large categories of angiosperms, owing to their conserved domain and three-dimensional structures. The WRKY transcription factors mediate plant adaptation to various environments via participating in various biological pathways, such as ROS (reactive oxygen species) and hormone signaling pathways, further regulating plant enzyme systems, stomatal closure, and leaf shrinkage physiological responses. This article analyzed the evolution of the WRKY family in angiosperms and its functions in responding to various external environments, especially the function and evolution in Magnoliaceae plants. It helps to gain a deeper understanding of the evolution and functional diversity of the WRKY family and provides theoretical and experimental references for studying the molecular mechanisms of environmental stress.

show abstract

“…For WRKY57 , the first intron is nearly entirely deleted in both XIS cucumbers (XIS49 and BN80), which results in expression suppression ( Figure 3 ). WRKY57 positively regulates drought tolerance and cold tolerance in Arabidopsis [ 40 , 41 , 42 ]. The ABHD6 gene has 10 CDS in CL cucumber, but the 9th intron and the 10th CDS are lost in both XIS cucumbers.…”

Section: Discussionmentioning

confidence: 99%

Lipid-Related Domestication Accounts for the Extreme Cold Sensitivity of Semiwild and Tropic Xishuangbanna Cucumber (Cucumis sativus L. var. xishuangbannanesis)

Zhang,

Liu,

Song

et al. 2023

IJMS

View full text Add to dashboard Cite

Xishuangbanna (XIS) cucumber (Cucumis sativus L. var. xishuangbannanesis) is a semiwild variety originating from low latitude tropic areas, and therefore shows extreme cold sensitivity and heat tolerance. Here, we mapped the quantitative trait loci (QTLs) that control the cold sensitivity and heat tolerance of XIS cucumber seedlings. Using bulked segregant analysis (BSA), we identified three QTLs (HTT1.1, HTT3.1, and HTT3.2, with a total length of 11.98 Mb) for heat tolerance and two QTLs (LTT6.1 and LTT6.2, with a total length of 8.74 Mb) for cold sensitivity. The QTL LTT6.1 was then narrowed down to a length of 641 kb by using kompetitive allele-specific PCR (KASP) markers. Based on structural variants (SVs) and single-nucleotide polymorphisms (SNPs), we found the LTT6.1 is covered by a high divergent region including a 50 kb deletion in the XIS49 genome, which affects the gene structure of lipase abhydrolase domain containing 6 (ABHD6, Csa_6G032560). Accordingly, there is a very big difference in lipid composition, but not in other osmoprotectants like free amino acids and fatty acids, between XIS49 and cultivated cucumber CL. Moreover, we calculated the composite likelihood ratio (CLR) and identified selective sweeps from 115 resequencing data, and found that lipid- and fatty-acid-related processes are major aspects in the domestication of the XIS group cucumber. LTT6.1 is a particularly special region positioned nearby lipid-related selective sweeps. These studies above suggested that the lipid-related domestication of XIS cucumbers should account for their extreme cold sensitivity.

show abstract

A WRKY Transcription Factor PmWRKY57 from Prunus mume Improves Cold Tolerance in Arabidopsis thaliana

Cited by 18 publications

References 32 publications

Time-Series Transcriptome Analysis Reveals the Molecular Mechanism of Ethylene Reducing Cold Sensitivity of Postharvest ‘Huangguan’ Pear

Time-Series Transcriptome Analysis Reveals the Molecular Mechanism of Ethylene Reducing Cold Sensitivity of Postharvest ‘Huangguan’ Pear

Evolution of the WRKY Family in Angiosperms and Functional Diversity under Environmental Stress

Lipid-Related Domestication Accounts for the Extreme Cold Sensitivity of Semiwild and Tropic Xishuangbanna Cucumber (Cucumis sativus L. var. xishuangbannanesis)

Contact Info

Product

Resources

About