A Work-Performing Heart Preparation for Myocardial Performance Analysis in Murine Hearts

Bittner, Hartmuth B.; Chen, Edward P.; Peterseim, David S.; Trigt, Peter Van

doi:10.1006/jsre.1996.0306

Cited by 15 publications

(21 citation statements)

References 2 publications

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“…The values of CF found in the present study under baseline loading conditions are comparable to those described previously [4,[9][10][11]23]. The observation that CF could increase by an additional 50% during the construc-188 and LV dP/dt min were 80±16 mm Hg, 5100 ±1617 mm Hg s -1 and -2885±476 mm Hg s -1 , respectively.…”

Section: Effect Of Ca 2+ On Cardiac Functionsupporting

confidence: 91%

“…In the present study CO increased from about 12 to 19 ml min -1 when preload was increased from 10 to 25 mm Hg at a fixed afterload of 50 mm Hg. These cardiac output values are close to those (16 ml min -1 ) found in mice in vivo [2,12] and substantially higher than the CO values of 1-4 ml min -1 reported by Bittner et al [4] with comparable loading conditions. A comparison with the functional characteristics reported by the group of Grupp [11,23] is more difficult as these investigators normally used a fixed left atrial inflow instead of a fixed filling pressure.…”

Section: Effect Of Ca 2+ On Cardiac Functionsupporting

confidence: 82%

“…The development of such a model, however, is not without problems. Haemodynamic function in terms of cardiac output and contractility of the isolated ejecting mouse heart reported in previous studies [4,9,23] differs markedly from the performance reported in the intact mouse [2,12,13,15,17,20].…”

Section: Introductioncontrasting

confidence: 70%

“…With the present experimental set-up and using loading conditions comparable to those reported by the groups of Bittner et al [4], Grupp et al [11,23] and Gauthier et al [9,10], the haemodynamic function of the isolated, working, mouse heart is relatively stable for at least 100 min: CO decreased by less than 10% over this period. More importantly, the haemodynamic performance in terms of CO and contractility compares favourably with the in vivo situation, in contrast to the other studies.…”

Section: Effect Of Ca 2+ On Cardiac Functionsupporting

confidence: 50%

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An improved isolated, left ventricular ejecting, murine heart model

Windt

Willems

Reneman

et al. 1999

Pfl�gers Archiv European Journal of Physiology

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An improved, isolated, left ventricular-ejecting, murine heart model is described and evaluated. Special attention was paid to the design and impedance characteristics of the artificial aortic outflow tract and perfusate composition, which contained glucose (10 mM plus insulin) and pyruvate (1.5 mM) as substrates. Temperature of the isolated perfused hearts was maintained at 38.5 degrees C. During antegrade perfusion (preload 10 mm Hg, afterload 50 mm Hg, 2.5 mM Ca2+) proper design of the aortic outflow tract provided baseline values for cardiac output (CO), left ventricular developed pressure (LVDP) and the maximum first derivative of left ventricular pressure (LV dP/dtmax) of 11.1+/-1.7 ml min-1, 83+/-5 mm Hg and 6283+/-552 mm Hg s-1, respectively, resembling findings in the intact mouse. During 100 min normoxic antegrade perfusion CO declined non-significantly by less than 10%. Varying pre- and afterloads resulted in typical Frank-Starling relationships with maximal CO values of 18.6+/-1.8 ml min-1 at pre- and afterload pressures of 25 and 50 mm Hg, respectively. Left ventricular function curves were constructed at free [Ca2+] of 1.5 and 2.5 mM in the perfusion medium. Significantly higher values for CO, LVDP and LV dP/dtmax and LV dP/dtmin were obtained at 2.5 mM Ca2+ at all loading conditions investigated. Phosphocreatine and creatine levels remained stable throughout the perfusion period. Despite a small but significant decline in tissue ATP content, the sum of adenine nucleotides did not change during the normoxic perfusion period. The tissue content of glycogen increased significantly.

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Section: Effect Of Ca 2+ On Cardiac Functionsupporting

confidence: 91%

Section: Effect Of Ca 2+ On Cardiac Functionsupporting

confidence: 82%

Section: Introductioncontrasting

confidence: 70%

Section: Effect Of Ca 2+ On Cardiac Functionsupporting

confidence: 50%

See 2 more Smart Citations

An improved isolated, left ventricular ejecting, murine heart model

Windt

Willems

Reneman

et al. 1999

Pfl�gers Archiv European Journal of Physiology

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show abstract

“…The heart and aorta were dissected free and perfused in the Langendorff mode at 37.5°C with a (retrograde) perfusion pressure of 100 cm H 2 O. The preparation was then converted to a working mode with a left atrial pressure of 15 cm H 2 O and an anterograde perfusion pressure of 80 cm H 2 O 11,12 (online data supplement).…”

Section: Hemodynamic Studies (Working Heart Preparation)mentioning

confidence: 99%

Adaptive Mechanisms That Preserve Cardiac Function in Mice Without Myoglobin

Meeson

Radford

Shelton

et al. 2001

Circulation Research

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Abstract-Mice lacking myoglobin survive to adulthood and meet the circulatory demands of exercise and pregnancy without cardiac decompensation. In the present study, we show that many myoglobin-deficient embryos die in utero at midgestation with signs of cardiac failure. Fetal mice that survive to gestational day 12.5, however, suffer no subsequent excess mortality. Survival in the absence of myoglobin is associated with increased vascularity and the induction of genes encoding the hypoxia-inducible transcription factors 1␣ and 2, stress proteins such as heat shock protein 27, and vascular endothelial growth factor. These adaptations are evident in late fetal life, persist into adulthood, and are sufficient to maintain normal myocardial oxygen consumption during stressed conditions. These data reveal that myoglobin is necessary to support cardiac function during development, but adaptive responses evoked in some animals can fully compensate for the defect in cellular oxygen transport resulting from the loss of myoglobin.

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Cardiovascular activity1

Vogel

Schölkens

et al. 2002

Drug Discovery and Evaluation

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A Work-Performing Heart Preparation for Myocardial Performance Analysis in Murine Hearts

Cited by 15 publications

References 2 publications

An improved isolated, left ventricular ejecting, murine heart model

An improved isolated, left ventricular ejecting, murine heart model

Adaptive Mechanisms That Preserve Cardiac Function in Mice Without Myoglobin

Cardiovascular activity1

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