A widely useful chiral stationary phase for the high-performance liquid chromatography separation of enantiomers

Pirkle, William H.; Finn, John M.; Schreiner, J.L.; Hamper, Bruce C.

doi:10.1021/ja00403a076

Cited by 342 publications

(113 citation statements)

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“…The HPLC methods using chiral stationary phases (Pirkletype columns, [47][48][49] cyclodextrin bonded columns 50,51) ) are also useful. In the present article, a reversed-phase HPLC method using precolumn chiral derivatization with OPA and N-tert-butyloxycarbonyl-L-cysteine (Boc-L-Cys) is described as a simultaneous determination method for D-Asp, D-Ser and D-Ala in mammalian tissues.…”

Section: Analytical Methods For the Determina-tion Of D-amino Acids Imentioning

confidence: 99%

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Hamase

Konno

Morikawa

et al. 2005

Biological & Pharmaceutical Bulletin

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Section: Analytical Methods For the Determina-tion Of D-amino Acids Imentioning

confidence: 99%

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Hamase

Konno

Morikawa

et al. 2005

Biological & Pharmaceutical Bulletin

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“…The principle was introduced by Pirkle [43,44]. The first phase developed in this group contained (R)-N-(3,5-dinitrobenzoyl)phenylglycine (R)-N-DNBPG as a chiral selector with p-acceptor properties.…”

Section: Direct Methodsmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

223

135

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This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

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“…To date, CSPs with several chiral selectors, such as cyclodextrins [1], macrocyclic antibiotics [2], polysaccharide derivatives [3], modified amino acids [4], proteins [5] and cinchona alkaloids [6], have been reported and some of them are commercially available. We recently developed new series of the CSPs having poly(L-Phe) peptide selector and successfully applied for chiral separations of some pharmaceuticals in reversed-and normal-phase modes [7,8].…”

Section: Introductionmentioning

confidence: 99%

Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly(l-phenylalanine) Chiral Stationary Phase

et al. 2011

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Characteristic of chiral stationary phase (CSP) with poly(L-phenylalanine) peptide selector, which is in -helical state, was reported. Since environmental factors affect peptide conformation, the changes of enantioselectivity were examined depending on column temperature and mobile phase conditions (ionic strength, pH, mobile phase composition). Column temperature and pH drastically affected the enantioselectivity.Based on these changes, the relation between chiral recognition and secondary structure of the peptide selector was discussed. The column stability during sequential analysis under different separation conditions was also evaluated.

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A widely useful chiral stationary phase for the high-performance liquid chromatography separation of enantiomers

Cited by 342 publications

References 0 publications

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Chiral separation by chromatographic and electromigration techniques. A Review

Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly(l-phenylalanine) Chiral Stationary Phase

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