A wide range of carbohydrate modifications by a single micro-organism: leuconostoc mesenteroides

Soetaert, Wim; Schwengers, Dieter; Buchholz, Klaus; Vandamme, Erick

doi:10.1016/s0921-0423(06)80116-4

Cited by 19 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dextransucrases from Leuconostoc mesenteroides have been widely used in the pharmaceutical and alimentary industries since the last century. Dextran produced by L. mesenteroides NRRL B-512F (ATCC 10830A) was one of the first biopolymers to be produced on an industrial scale [15], finding several applications in medicine, separation technology, and biotechnology [14,40]. Leuconostoc spp.…”

Section: Introductionmentioning

confidence: 99%

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

Vidal¹,

Martínez²,

Moulis³

et al. 2011

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

The industrial Leuconostoc strain B/110-1-2 producing dextran and dextran derivatives was taxonomically identified by 16S rRNA as L. citreum. Its dextransucrase enzymes were characterized according to their cellular location and reaction specificity. In the presence of sucrose, the strain B/110-1-2 produced two cell-associated dextransucrases (31.54% of the total glucosyltransferase activity) with molecular weights of 160 and 240 kDa and a soluble dextransucrase (68.46%) at 160-180 kDa. Two open reading frames (ORF) coding for L. citreum strain B/110-1-2 dextransucrases were identified. One of them shared a 52% identity with the alternansucrase ASR of L. citreum NRRL B-1355 and with a putative annotated alternansucrase sequence found in the genome of L. citreum KM20. The structural analysis (HPAEC-PAD, HPSEC, and (13)C-NMR) of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases suggest this novel glucansucrase has specificity similar to a dextransucrase but not to an alternansucrase, producing a soluble linear dextran with glucose molecules linked mainly in α-1,6 and α-1,3 with α-1,4 branches. These results enhance the understanding of this industrially significant strain and will aid in distinguishing between physiologically similar Leuconostoc spp. strains.

show abstract

Section: Introductionmentioning

confidence: 99%

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

Vidal¹,

Martínez²,

Moulis³

et al. 2011

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…21 Also, the final result is a poor mannitol yield, with only approximately 15% crystalline mannitol, and in this process, mannitol is the only by-product in a reaction that produces mostly sorbitol; this makes mannitol production dependent on the sorbitol market and price. 12 Various authors have lately proven that heterofermentative lactic acid bacteria are able to convert D-fructose into D-mannitol under mild conditions, [21][22][23][24][25] whereas cyanobacteria have been considered a mannitol producer. 26…”

Section: Mannitol Productionmentioning

confidence: 99%

The use of mannitol in neurosurgery and neuro-ophthalmology

Turliuc¹,

Cucu²,

Costăchescu³

et al. 2019

Cellulose Chem. Technol.

View full text Add to dashboard Cite

“…However, only a few such applications have progressed to the industrial scale to date. The linear dextran produced by L. mesenteroides NRRL B-512F has been used as a blood plasma substitute (Alsop 1984) and is now exploited for use in chromatographic supports and also as an iron carrier (Soetaert et al 1995). This dextran has also recently been approved as an additive in panification (Vandamme et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Glucansucrases of GH family 70: What are the determinants of their specifities?

Fabre¹,

Joucla²,

Moulis³

et al. 2006

Biocatalysis and Biotransformation

View full text Add to dashboard Cite

Glucansucrases from family 70 of glycoside-hydrolases catalyse the synthesis of a-glucans with various types of osidic linkages from sucrose. Among these enzymes, alternansucrase (ASR) and dextransucrase E (DSR-E) catalyse the formation of unusual a-glucans. ASR catalyses the synthesis of linear glucan with a-1,3 and a-1,6 alternating linkages and DSR-E synthesizes a glucan containing a-1,6 linkages in the linear chain and a-1,2 branches. The sequence analysis of these enzymes enabled the identification of structural elements suspected to be involved in the enzyme specificities. Biochemical characterization of ASR and DSR-E variants obtained from gene truncations or site-directed mutagenesis experiments showed that the specificity of these enzymes to form different types of osidic linkage is controlled by two different approaches. For ASR, the double specificity is controlled by only one catalytic domain where important amino acids involved in the enzyme specificity have been identified. In the case of DSR-E, the double specificity is controlled by two different catalytic domains both belonging to family 70, each domain being specific of one type of linkage.

show abstract

A wide range of carbohydrate modifications by a single micro-organism: leuconostoc mesenteroides

Cited by 19 publications

References 12 publications

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

The use of mannitol in neurosurgery and neuro-ophthalmology

Glucansucrases of GH family 70: What are the determinants of their specifities?

Contact Info

Product

Resources

About