A wide diversity of bacteria from the human gut produces and degrades biogenic amines

Pugin, Benoı̂t; Barcik, Weronika; Westermann, Patrick; Heider, Anja; Wawrzyniak, Marcin; Hellings, Peter W.; Akdiş, Cezmi A.; O’Mahony, Liam

doi:10.1080/16512235.2017.1353881

Cited by 126 publications

(132 citation statements)

References 44 publications

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“…Of recent interest are studies showing that gut bacteria in humans are able to produce other metabolites with pro-and anti-inflammatory potential, such as biogenic amines (including histamine) (Pugin et al, 2017) and oxylipins such as 12,13-di-HOME (Levan et al, 2019). The number of histamine-secreting bacteria is significantly higher in fecal samples of asthma patients compared with non-asthmatic volunteers .…”

Section: Bacteria Are the Largest Microbial Population In The Gut Andmentioning

confidence: 99%

The Role of Lung and Gut Microbiota in the Pathology of Asthma

et al. 2020

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Section: Bacteria Are the Largest Microbial Population In The Gut Andmentioning

confidence: 99%

The Role of Lung and Gut Microbiota in the Pathology of Asthma

et al. 2020

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“…Roeder and Schink described a new strain close to Clostridium aminobutyricum , able to degrade cadaverine, in co-culture with the archaea Methanospirullum (Roeder and Schink, 2009). On the other hand, some Clostridium were also identified to be involved in the histamine degradation (Pugin et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Integrative analyses to investigate the link between microbial activity and metabolites degradation during anaerobic digestion

Cardona¹,

Cao²,

Puig‐Castellví

et al. 2020

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Tel + 33 1 40 96 65 06 16 Fax + 33 1 40 96 61 99 17 olivier.chapleur@irstea.fr 18 https://orcid.org/0000-0001-9460-921X 19 20 Abstract 21Anaerobic digestion (AD) is a promising biological process to convert waste into sustainable 22 energy. However, the microbiota involved in this bioprocess is complex and additional 23 knowledge is still needed to fully exploit its capability. High throughput methodologies open 24 new perspectives, but innovative data integration methodologies are required for extracting 25 relevant information from these rich data. We analysed the association between microbial 26 activity and the patterns of substrate degradation during a lab-scale co-digestion experiment. 27These parameters were longitudinally monitored using 16S rRNA sequencing and untargeted 28 metabolomics. In this experiment, samples were collected from digesters fed with 9 different 29 mixtures of fish, sewage sludge, and grass. Our objective was to identify microorganisms 30 2 responsible for the degradation of molecules specific of each co-substrate. Five main groups 31 of correlated features were successfully evidenced. For example, the degradation of 32 cadaverine was found to be correlated with microorganisms from the order Clostridiales and 33 the genus Methanosarcina, and the degradation of lignin compounds was correlated with 34 cellulolytic degraders Lactobacillales. This study highlights the potential of data integration 35 towards a comprehensive understanding of AD microbiota. 36 37 Keywords 38 16S RNA sequencing; metabolomic; data integration; methanisation; co-digestion; PLS 39 canonical 40 41 101 4°C until the incubation experiments.102 Bioreactors experimental set-up 103 Binary mixtures of sludge with linearly increasing (0-100, 25-75, 50-50, 75-25, 100-0) 104 percentages of either fish or grass were prepared (Figure 1). Experiments were carried out in 105 51L glass bottles (700 mL working volume) at 35°C in the dark without agitation. The same 106 quantity of carbon was added in all the digesters, and the ratio of substrate/inoculum used to 107 feed and inoculate all the digesters was fixed at 12 gCOD/1.2 gCOD (Table S1). All the 108 bioreactors were complemented with a biochemical potential buffer (International Standard 109 ISO 11734 (1995)) to reach a final working volume of 700 mL. All incubations were 110 performed in triplicate. The bioreactors were then sealed with a screw cap and a rubber 111 septum and the headspaces were then flushed with N 2 (purity >99.99%, Linde gas SA). In 112 total 27 anaerobic bioreactors were set-up. 113Weekly (at days 0, 7, 14, 21), for every reactor, 6 mL of liquid phase were sampled 114 through the septum using a syringe. The collected samples were centrifuged at 10 000g for 10 115 minutes to separate the supernatants from the pellets. Supernatant were snap frozen using 116 liquid nitrogen and kept at -20°C for metabolomic analysis and pellets kept at -80°C for 117 microbial analysis. 118 119 RNA extraction and 16S rRNA sequencing 126Based on the biogas production, a t...

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“…We infer that JUb39 TyrDC likely generates TA from tyrosine. Morganella strains (Morganellaceae family) have been reported to produce TA under certain conditions 28 , despite having no discernible tyrDC orthologs (Fig. 3c, Fig.…”

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Modulation of sensory behavior and food choice by an enteric bacteria-produced neurotransmitter

O’Donnell¹,

Fox

Chao³

et al. 2019

Preprint

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Animals coexist in commensal, pathogenic or mutualistic relationships with complex communities of diverse organisms including microbes 1 . Some bacteria produce bioactive neurotransmitters which have been proposed to modulate host nervous system activity and behaviors 2 . However, the mechanistic basis of this microbiota-brain modulation and its physiological relevance is largely unknown. Here we show that in C. elegans, the neuromodulator tyramine (TA) produced by gut-colonizing commensal Providencia bacteria can bypass the requirement for host TA biosynthesis to manipulate a host sensory decision. Bacteriallyproduced TA is likely converted to octopamine (OA) by the host tyramine beta-hydroxylase enzyme. OA, in turn, targets the OCTR-1 receptor on the ASH/ASI sensory neurons to modulate an aversive olfactory response. We identify genes required for TA biosynthesis in Providencia, and show that these genes are necessary for modulation of host behavior. We further find that C. elegans colonized by Providencia preferentially select these bacteria in food choice assays, and that this selection bias requires bacterially-produced TA. Our results demonstrate that a neurotransmitter produced by gut microbiota mimics the functions of the cognate host molecule to override host control of a sensory decision, thereby promoting fitness of both host and microbe.

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A wide diversity of bacteria from the human gut produces and degrades biogenic amines

Cited by 126 publications

References 44 publications

The Role of Lung and Gut Microbiota in the Pathology of Asthma

The Role of Lung and Gut Microbiota in the Pathology of Asthma

Integrative analyses to investigate the link between microbial activity and metabolites degradation during anaerobic digestion

Modulation of sensory behavior and food choice by an enteric bacteria-produced neurotransmitter

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