A Whole-Genome Radiation Hybrid Map of the Dog Genome

Priat, Catherine; Hitte, Christophe; Vignaux, Françoise; Renier, Corinne; Jiang, Zhihua; Jouquand, Sophie; Chéron, Angélique; André, Catherine; Galibert, Francis

doi:10.1006/geno.1998.5602

Cited by 122 publications

(108 citation statements)

References 49 publications

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“…The sequence assembly validated much of what was already hypothesized from comparative genome map studies. The genome is approximately 2.4 billion bases and is comprised of approximately 250 conserved segments when compared to the human genome 16,17,21,[65][66][67] . The assembled sequence is estimated to cover 98% of the dog genome with the majority of genes containing no sequence gaps.…”

Section: Canine Genome Maps and The Canine Genome Sequencementioning

confidence: 99%

“…Recent studies have brought advances in understanding the genetics of breed development 15 and the availability of ever higher resolution maps [16][17][18][19][20][21] . These have led to the mapping of disease loci for a variety of diseases including metabolic disorders 22,23 , blindness [24][25][26][27][28] , cancer 29,30 neurologic disorders 31,32 , hip dysplasia 33 epilepsy 34 , as well as several morphologic traits 35,36 .…”

Section: Introductionmentioning

confidence: 99%

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Finding cardiovascular disease genes in the dog

Parker

Meurs

Ostrander

2006

Journal of Veterinary Cardiology

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Recent advances in canine genomics are changing the landscape of veterinary biology, and by default, veterinary medicine. No longer are clinicians locked into traditional methods of diagnoses and therapy. Rather major advances in canine genetics and genomics from the past five years are now changing the way the veterinarian of the 21 st century practices medicine.First, the availability of a dense genome map gives canine genetics a much needed foothold in comparative medicine, allowing advances made in human and mouse genetics to be applied to companion animals. Second, the recently released 7.5x whole genome sequence of the dog is facilitating the identification of hereditary disease genes. Finally, development of genetic tools for rapid screening of families and populations at risk for inherited disease means that the cost of identifying and testing for disease loci will significantly decrease in coming years.Out of these advances will come major changes in companion animal diagnostics and therapy. Clinicians will be able to offer their clients genetic testing and counseling for a myriad of disorders. Such advances are certain to generate healthier and more long lived dogs, improving quality of life for owner and pet alike. The clinician of the 21 st century, therefore, faces incredible opportunities as well as challenges in the management of genetic disease. In this review we summarize recent findings in canine genomics and discuss their application to the study of canine cardiac health.

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Section: Canine Genome Maps and The Canine Genome Sequencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Finding cardiovascular disease genes in the dog

Parker

Meurs

Ostrander

2006

Journal of Veterinary Cardiology

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“…C'est ainsi que nous avons construit une collection d'hybrides cellulaires [30] et avons isolé et caractérisé plusieurs centaines de marqueurs canins [31][32][33]. Ce panel RH constitué de 118 lignées indépendantes résultant de la fusion aléatoire de fibroblastes canins irradiés à 5 000 rads et de cellules de hamster porteuses d'une mutation dans le gène de la thymidine kinase a permis la construction de cartes de plus en plus riches en marqueurs polymorphes (microsatellites) ou en gènes [34][35][36][37]. Ce panel RH, distribué à la communauté internationale s'intéressant à la génétique canine, sert actuellement de référence pour la cartographie de ce génome ainsi que pour la localisation de gènes candidats ou impliqués dans des anomalies génétiques spécifiques de races.…”

Section: Analyse Structurale Du Génome Caninunclassified

“…Les arguments développés en faveur de cette analyse étaient de trois ordres: l'interprétation de la séquence du génome humain, maintenant disponible dans sa version définitive (version 34 …”

Section: Séquençage Du Génome Caninunclassified

Le chien, un modèle pour la génétique des mammifères

2004

Self Cite

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“…It was Walter et al (5) who returned to the original Goss and Harris protocols and presented a panel of cell hybrids that they had constructed by fusion of irradiated diploid human fibroblasts with hamster cells. Since then, whole-genome RH panels have been constructed and characterized for a variety of mammalian species other than human, including pig (6,7), cow (8), rat (9,10), dog (11,12), cat (13) and mouse (14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Interspersed repetitive sequence (IRS)-PCR for typing of whole genome radiation hybrid panels

Himmelbauer

Schalkwyk

Lehrach

2000

Nucleic Acids Research

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The typing of a radiation hybrid (RH) panel is generally achieved using a unique primer pair for each marker. We here describe a complementing approach utilizing IRS-PCR. Advantages of this technology include the use of a single universal primer to specify any locus, the rapid typing of RH lines by hybridization, and the conservative use of hybrid DNA. The technology allows the mapping of a clone without the requirement for STS generation. To test the technique, we have mapped 48 BAC clones derived from mouse chromosome 12 which we mostly identified using complex probes. As mammalian genomes are repeat-rich, the technology can easily be adapted to species other than mouse.

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A Whole-Genome Radiation Hybrid Map of the Dog Genome

Cited by 122 publications

References 49 publications

Finding cardiovascular disease genes in the dog

Finding cardiovascular disease genes in the dog

Le chien, un modèle pour la génétique des mammifères

Interspersed repetitive sequence (IRS)-PCR for typing of whole genome radiation hybrid panels

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