A Wheat R2R3-type MYB Transcription Factor TaODORANT1 Positively Regulates Drought and Salt Stress Responses in Transgenic Tobacco Plants

Wei, Qiuhui; Luo, Qingchen; Wang, Ruibin; Zhang, Fan; He, Yuan; Zhang, Yang; Qiu, Ding; Li, Kexiu; Chang, Junli; Yang, Guangxiao; He, Guoqing

doi:10.3389/fpls.2017.01374

Cited by 114 publications

(72 citation statements)

References 60 publications

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“…Soil salinization is a serious threat to the environment and agricultural productivity worldwide. Research into salt tolerance and its underlying mechanisms has been carried out in many plant species, such as rice [1,2], melon [3], maize [4], tobacco [5] and tomato [6]. Investigations of plant salt tolerance are crucial for the sustainable development of saline agriculture.…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

et al. 2019

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Background Clerodendrum inerme (L.) Gaertn, a halophyte, usually grows on coastal beaches as an important mangrove plant. The salt-tolerant mechanisms and related genes of this species that respond to short-term salinity stress are unknown for us. The de novo transcriptome of C. inerme roots was analyzed using next-generation sequencing technology to identify genes involved in salt tolerance and to better understand the response mechanisms of C. inerme to salt stress. Results Illumina RNA-sequencing was performed on root samples treated with 400 mM NaCl for 0 h, 6 h, 24 h, and 72 h to investigate changes in C. inerme in response to salt stress. The de novo assembly identified 98,968 unigenes. Among these unigenes, 46,085 unigenes were annotated in the NCBI non-redundant protein sequences (NR) database, 34,756 sequences in the Swiss-Prot database and 43,113 unigenes in the evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) database. 52 Gene Ontology (GO) terms and 31 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were matched to those unigenes. Most differentially expressed genes (DEGs) related to the GO terms “single-organism process”, “membrane” and “catalytic activity” were significantly enriched while numerous DEGs related to the plant hormone signal transduction pathway were also significantly enriched. The detection of relative expression levels of 9 candidate DEGs by qRT-PCR were basically consistent with fold changes in RNA sequencing analysis, demonstrating that transcriptome data can accurately reflect the response of C. inerme roots to salt stress. Conclusions This work revealed that the response of C. inerme roots to saline condition included significant alteration in response of the genes related to plant hormone signaling. Besides, our findings provide numerous salt-tolerant genes for further research to improve the salt tolerance of functional plants and will enhance research on salt-tolerant mechanisms of halophytes.

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Section: Introductionmentioning

confidence: 99%

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

et al. 2019

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“…The MYB transcription factor contains a MYB domain that is highly conserved across all eukaryotes 116 . Numerous studies have suggested that MYB proteins are also involved in regulating plant responses to biotic and abiotic stresses [121][122][123] . Overexpression of TaODORANT1 enhances resistance to drought and salt stress in transgenic plants 123 .…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have suggested that MYB proteins are also involved in regulating plant responses to biotic and abiotic stresses [121][122][123] . Overexpression of TaODORANT1 enhances resistance to drought and salt stress in transgenic plants 123 . Moreover, PbrMYB21 gene by regulating of polyamine synthesis pathway plays a role in drought tolerance mechanisms of plants 124 .…”

Section: Discussionmentioning

confidence: 99%

A genome-wide identification, characterization and functional analysis of salt-related long non-coding RNAs in non-model plant Pistacia vera L. using transcriptome high throughput sequencing

Jannesar

Seyedi

Jazi

et al. 2020

Sci Rep

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Long non-coding RNAs (lncRNAs) play crucial roles in regulating gene expression in response to plant stresses. Given the importance regulatory roles of lncRNAs, providing methods for predicting the function of these molecules, especially in non-model plants, is strongly demanded by researchers. Here, we constructed a reference sequence for lncRNAs in P. vera (Pistacia vera L.) with 53220 transcripts. In total, we identified 1909 and 2802 salt responsive lncRNAs in Ghazvini, a salt tolerant cultivar, after 6 and 24 h salt treatment, respectively and 1820 lncRNAs in Sarakhs, a salt sensitive cultivar, after 6 h salt treatment. Functional analysis of these lncRNAs by several hybrid methods, revealed that salt responsive NAT-related lncRNAs associated with transcription factors, CERK1, LEA, Laccase genes and several genes involved in the hormone signaling pathways. Moreover, gene ontology (GO) enrichment analysis of salt responsive target genes related to top five selected lncRNAs showed their involvement in the regulation of ATPase, cation transporter, kinase and UDP-glycosyltransferases genes. Quantitative real-time PCR (qRT-PCR) experiment results of lncRNAs, pre-miRNAs and mature miRNAs were in accordance with our RNA-seq analysis. In the present study, a comparative analysis of differentially expressed lncRNAs and microRNA precursors between salt tolerant and sensitive pistachio cultivars provides valuable knowledge on gene expression regulation under salt stress condition. Pistacia belongs to Anacardiaceae family and contains 13 or more species, which among them pistachio (Pistacia vera L.) is the only cultivated and economically important species that is called as the 'green gold tree'. The other species are used mainly as a rootstock for pistachio cultivation 1,2. Like many other fruit trees, P. vera is hard to root and thus requires a rootstock for vegetative propagation 3. Pistachio is a deciduous (2n = 30), wind-pollinated tree species 4,5. Studies indicate that pistachio probably originated in the Middle East 6-8. It has a long history of cultivation (3000-4000 years) in Iran 9. In 2017, Iran has been ranked first in area harvested (429535 ha) and production quantity (574987 tons) of pistachio among United States, Turkey, and Syria as biggest pistachio producer countries 10. According to the number of pistachio genotypes, Iran is one of the rich countries in the world 7,8,11. In addition to fruit, other parts of plant, including leaf, flower and resins, have pharmacological properties 12,13. Salinity is major and ever-increasing problem in arid and semi-arid regions that affect plant production and growth throughout the world 14. The cultivated areas of pistachio in Iran are often in arid and semi-arid regions that the most important problem for economic crop production in these areas is high concentration of ions

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“…MYBs presented similar patterns and conserved motifs, suggesting that their conserved features play similar roles in group-specific functions. As part of a myb-bHLH-WD40 complex, they are involved in plant secondary metabolism and responses to abiotic and biological stresses [24,25]. Several R2R3 MYBs were analyzed for their possible involvement in anthocyanin biosynthesis and accumulation, and fructan exohydrolase regulation when subjected to different stress and hormone treatments [26,27].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Rosa roxbunghii Tratt transcriptome and analysis of MYB genes

Yan

Huang

Zhai

et al. 2018

Preprint

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16Rosa roxbunghii Tratt belongs to the Rosaceae family, and the fruit is flavorful, 17 economic, and highly nutritious, providing health benefits. MYB proteins play key roles 18 in R. roxbunghii' fruit development and quality. However, the available genomic and (37,545/63,727). This transcriptome data will be valuable for 33 identifying genes of interest and studying their expression and evolution.

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A Wheat R2R3-type MYB Transcription Factor TaODORANT1 Positively Regulates Drought and Salt Stress Responses in Transgenic Tobacco Plants

Cited by 114 publications

References 60 publications

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

A genome-wide identification, characterization and functional analysis of salt-related long non-coding RNAs in non-model plant Pistacia vera L. using transcriptome high throughput sequencing

Characterization of the Rosa roxbunghii Tratt transcriptome and analysis of MYB genes

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