A water-soluble two-photon fluorescent probe for rapid and reversible monitoring of redox state

Methylthio‐substituted coumarin‐based fluorescent probe for highly specific and fast detection of hypochlorite and bioimaging application

“…The recently developed hemicyanine‐based fluorescent probe showed excellent efficiency for simultaneous detection of ClO − and viscosity 27 . Selenium‐containing 1,8‐naphthalimide‐derived probe NA‐Se served a nearly perfect example for rapid and reversible fluorescence turn‐on monitoring of ClO − , were it not for the slight fluorescence enhancement caused by several other ROS including H 2 O 2 , ONOO − , • OH and 1 O 2 during the sensing process 35 . Overall, probe MOMTC displayed unique advantages for fulfilling multiple requirements needed by an efficient hypochlorite fluorescent probe: (1) high specificity toward hypochlorite without any interference from other common ROS analytes; (2) rapid response to hypochlorite within 30 s; (3) long wavelength fluorescence emission at 555 nm; and (4) high stability at pH 3–10 and a broad applicable range of pH 3–8.…”

“…27 Seleniumcontaining 1,8-naphthalimide-derived probe NA-Se served a nearly perfect example for rapid and reversible fluorescence turn-on monitoring of ClO À , were it not for the slight fluorescence enhancement caused by several other ROS including H 2 O 2 , ONOO À , • OH and 1 O 2 during the sensing process. 35 Overall, probe MOMTC displayed unique advantages for fulfilling multiple requirements needed by an efficient hypochlorite fluorescent probe:…”

“…In this regard fluorescent chemosensors receive extensive attention and application in view of their high sensitivity and selectivity, and temporal and spatial resolution for real-time imaging. [12][13][14][15][16][17][18] The design of ClO À fluorescent probes is mainly based on the selective oxidation of specific response groups by ClO À , including C=N double bond, [19][20][21][22][23][24][25][26] C=C double bond, [27][28][29][30] chalcogenide [sulphur (S) and selenium (Se)] atoms, [31][32][33][34][35] amido group, [36][37][38][39][40] p-aminophenol analogues, [41][42][43][44] thiocarbamate moiety, [45][46][47] aryl boronate group, 48,49 carbonsulphur bond 50,51 and several other bonds or groups. [52][53][54] By far people have synthesised a variety of fluorescent probes derived from different fluorophore platforms that specifically detect ClO À in living systems, achieving encouraging advancement in this field.…”

A phenothiazine and semi-cyanine based colorimetric and fluorescent probe for the rapid detection of hypochlorous acid

“…[14][15][16] These probes can be structurally divided into the following categories: (1) oxidation of CQC 17,18 or CQN 19,20 bonds; (2) oxidation of oximes 21,22 and hydrazines; 23,24 (3) oxidation of p-alkoxyaniline; 25,26 (4) oxidation of sulfur atoms [27][28][29][30][31][32][33][34][35] and some others. [36][37][38] By contrast (the properties of reported probes are listed in ESI † Table S1), sulfur atoms can be used in the construction of probes due to their various forms (thioethers, thioesters, thioacetals, etc.) and various reaction mechanisms with HOCl (shown in Scheme 1).…”

Construction of a Dual-Functional, Reversible, Ratiometric, Golgi-Targeting Fluorescent Probe for Real-time Monitoring of Dynamics of Intracellular Redox Homeostasis

“…As a result, fluorescent probes for monitoring the ClO – /GSH redox pair should be reversible. Recently, several reversible “off–on” probes for detection of the ClO – /GSH redox pair have been reported. − Despite their usefulness, these “off–on” probes are susceptible to the probe’s concentration and instrumental factors, which are detrimental to the performance and reliability of the bioimaging of the dynamic redox environments. Ratiometric fluorescent probes are more favorable than “off–on” probes because they have a higher accuracy during quantitative analysis and their operation is more simplified simply through self-calibration using two independent signals.…”