A water-soluble lysosome-targetable fluorescent probe for carboxylesterase detection and its application in biological imaging

Esterase Specific Fluorescent Probe: Mechanistic Understanding Using QM/MM Calculation and Cell States Discrimination

“…[9] Contrary, a ratiometric fluorescent probe can minimize errors due to the ratio of dual channel signals. [10,11] The reported esterase-specific probes are majorly localized to the cytosol and lysosomes, [3,[12][13][14][15] and very few probes are localized to mitochondria (Table S2). [16][17][18][19][20][21] Moreover, the hydrolysis mechanism and enzyme's active binding sites with the existing esterase probes were not explored systematically.…”

Tunable full-color emission of stilbazoles containing a 2-halo-3,4-dicyanopyridine acceptor

“…1). 19–45 This is due to the fact that the pyridine ring acts as an electron-withdrawing group in the push–pull chromophore system. 44,45 Moreover, stilbazoles are often characterized by facile preparation methods and a wide application range.…”

“…44,45 Moreover, stilbazoles are often characterized by facile preparation methods and a wide application range. For example, they can be used as non-linear optical materials (NLO), 19–22 light-absorbing components of dye-sensitized solar cells (DSSC), 23,24 organic light emitting diodes (OLED), 25,26 memory devices, 27 materials for two-photon absorption, 28,29 chemosensors, 30–36 fluorescent label probes 37–40 and dyes, 41–43 etc .…”

“…Thus, it should be noted that a huge amount of data on the synthesis and application of various heterocyclic styryl derivatives has been collected to date. 19–45,47–50 However, stilbazoles with a nicotinonitrile fragment remain less studied, and there are also no data about azastilbenes with a dicyanopyridine fragment in the literature.…”

Construction of a novel aminofluorene-based ratiometric near-infrared fluorescence probe for detecting carboxylesterase activity in living cells

“…Among numerous methods for analyzing CE activity, the uorescence imaging technique has attracted extensive attention due to its high sensitivity, noninvasive nature, high spatiotemporal resolution, etc. 11,12 Until now, many CE-specic response probes have been developed, which are usually based on the following uorophores: coumarin, 13 naphthalimide, [14][15][16] cyanine, 17 hemicyanine, [18][19][20] BODIPY, 21,22 rhodamine, 23 benzothiazole, 24,25 dicyanomethylene (DCM), 26 spiro-acridine, 27,28 avone, 29 diketopyrrolopyrrole (DTPP), 30 dicyanoisophorone (DCIP), 31,32 etc. However, most of them are "turn-on" uorescence signals with a single detection region, which is easily inuenced by some factors such as probe concentration, pH, temperature, and polarity.…”