A water-soluble boronate masked benzoindocyanin fluorescent probe for the detection of endogenous mitochondrial peroxynitrite in live cells and zebrafish as inflammation models

Endoplasmic Reticulum-Targeting Near-Infrared Fluorescent Probe for CYP2J2 Activity and Its Imaging Application in Endoplasmic Reticulum Stress and Tumor

“…ER stress was an abnormal response of ER toward the injury of external damage, which commonly occurred during various diseases. Herein, a classic DTT-induced ER stress model was established in HUVEC cells according to the previous reports. , Next, the regulation of endogenous CYP2J2 was imaged using ER-BnXPI ; briefly, after the DTT (2 mM) treatment for 6 h, ER-BnXPI (10 μM) was added into the cells and incubated for 30 min. After the staining, the cells were imaged as above mentioned.…”

“…Recently, the fluorescence technology has been widely used in the phototherapy, diagnosis, visual detection, and other fields. − The advantages of fluorescent probes such as noninvasive, real-time sampling capability, and sensitive and high spatiotemporal resolution make it much more convenient for the specific assay of target enzymes. − Among various fluorophores, the near-infrared (NIR) fluorescent probes exhibited some prominent advantages owing to its excellent tissue penetration and low background fluorescence, which allowed the visual detection of some vital metabolic enzymes for in vivo imaging. − …”

Affinity Studies of Hemicyanine Derived Water Soluble Colorimetric Probes with Reactive Oxygen/Nitrogen/Sulfur Species

“…In Table S1, we have compiled the literature on various water (100 %) soluble peroxynitrite selective probes (i. e., 0 % organic solvent requirement for the dissolution of the probe). [31,32,38,41,47,[55][56][57][58][59][60] There are not many water-soluble and peroxynitrite selective probes in the literature. Hence, further attention to the development of peroxynitrite specific and water-soluble probes is warranted.…”

Regulating the activity of boronate moiety to construct fluorescent probes for the detection of ONOO−in vitroandin vivo

“…, ONOO − , H 2 O 2 )-recognizing portion, with some developed fluorescent probes listed in Schemes S1–S3 (ESI†), the substituent effect has not been studied in the reported publications. 32–67 Resorufin dye possesses several advantages, including bright red emission, excellent stability, high fluorescence quantum yield, and good photostability. 68,69 Several boronate-containing resorufin probes have been reported to be able to detect tyrosinase, benzoyl peroxide (BPO), or hydrogen peroxide (H 2 O 2 ); however, the response rates were slow (10 min to 10 h, Table S1, ESI†).…”