A water extract of Samchulkunbi-tang attenuates airway inflammation by inhibiting inos and MMP-9 activities in an ovalbumin-induced murine asthma model

Abstract

BackgroundIn this study, we investigated the effect of Samchulkunbi-tang water extract (SCTE) in an established mouse model of ovalbumin (OVA)-induced allergic asthma. The effects of SCTE on the production of Th1 and Th2 cytokines, eotaxin, and total and OVA-specific immunoglobulin E, inducible nitric oxide synthase expression, and matrix metalloproteinase-9 activity were measured.MethodsMice were sensitized on days 0 and 14 with an intraperitoneal injection of 20 μg ovalbumin (OVA) emulsified in 2 mg aluminum hydroxide in 200 μL PBS buffer. On days 21, 22, and 23, mice received an airway exposure to OVA (1%, w/v, in PBS) for 1 h. SCTE was administered orally to mice at doses of 200 and 400 mg/kg per day from days 18 to 23.ResultsSCTE reduced the number of inflammatory cells, cytokines, and chemokines in bronchoalveolar lavage fluids and iNOS expression and MMP-9 activity in mouse lung tissue. Histological studies using hematoxylin & eosin and periodic acid-schiff staining showed that SCTE substantially inhibited OVA-induced inflammatory cell infiltration in lung tissue and goblet cell hyperplasia in the airway. SCTE also reduced IL-4 and IL-13 expression in concanavalin-A-stimulated splenocytes. These results were similar to those obtained with montelukast as a positive control.ConclusionsCollectively, these results suggest that SCTE may be an effective oral treatment for allergic airway inflammation by virtue of its anti-inflammatory activity.

Antiallergic Effects of Caffeic Acid in Blomia Tropicalis Murine Model of Experimental Asthma

“…31 IL-4 promotes the differentiation and proliferation of Th2-type T cells, and the switching of B cells to produce IgG1 and IgE iso types antibodies. 37 In the present study, the serum level of Bt-specific IgG1 in Bt-sensitized mice was significantly reduced by administration of CA. The production of IL-4, from isolated splenocytes incubated in the presence of PWM, was remarkably inhibited by the addition of CA, and the modulation on IgG1 could be related the decrease in IL-4.…”

Samsoeum water extract attenuates allergic airway inflammation via modulation of Th1/Th2 cytokines and decrease of iNOS expression in asthmatic mice

“…Our findings indicate that SSEW administration significantly decreased the increased Th2-type cytokines and chemokine production. Several studies have shown that modulation of Th1/Th2 cytokines can inhibit airway inflammation in OVA-induced mice [ 33 , 34 ]. Therefore, these findings suggest that SSEW attenuates airway inflammation through the modulation of the Th1/Th2 balance.…”

Preparation and evaluation of gastroretentive effervescent floating drug delivery system of Samchulkunbi-tang

“…Samchulkunbi-tang (ST) is a traditional herbal medicine widely used for treatment of chronic gastritis, gastric ulcers and gastroptosis. It is a composite traditional Korean medicine consisting basically of 14 herbs (Lee et al 2010(Lee et al , 2012. Pharmacological studies of ST have been confined to its roles in gastroprotection (Kim and Lee 1988) and immune regulation (Lee and Kim 1998).…”