A water activity control system for enzymatic reactions in organic media

Petersson, Anna E. V.; Adlercreutz, Patrick; Mattìasson, Bo

doi:10.1002/bit.21229

Cited by 44 publications

(44 citation statements)

References 40 publications

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“…As a consequence of these specifications, the preparation is limited by mass transfer under certain conditions, such as low substrate concentrations or high reaction rates. Novozym 1 435 has often been shown to work well or even best under dry conditions (Chamouleau et al, 2001;Petersson et al, 2007). It is clear from the results in the present study that this is not always true.…”

Section: Water Activity Dependence With Different Acyl Donorscontrasting

confidence: 58%

“…These reactions typically involve at least one hydrophobic substrate (Chamouleau et al, 2001;Petersson et al, 2007). It is noteworthy that low water activity optima occur even in hydrolytic reactions, as reported in a study of the hydrolysis of very hydrophobic esters using either Novozym 1 435 or Lipozyme RM IM as the catalyst.…”

Section: Water Activity Dependence With Different Acyl Donorsmentioning

confidence: 91%

“…Since the catalytic properties of enzymes are known to be highly dependent upon their hydration level, there is clear reason for studying enzymatic reactions at a variety of different water activity levels, allowing water activity profiles to be generated. Several previous studies using Novozym 1 435 have indicated that low water activity levels provide the highest rates of synthesis with this preparation (Petersson et al, 2007). These studies have mostly been made using straightchain, hydrophobic substrates, however.…”

Section: Introductionmentioning

confidence: 98%

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Efficient enzymatic acrylation through transesterification at controlled water activity

Nordblad¹,

Adlercreutz²

2007

Biotech & Bioengineering

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Enzymatic acrylation is a process of potentially strong interest to the chemical industry. Direct esterification involving acrylic acid is unfortunately rather slow, with inhibition phenomena appearing at high acid concentrations. In the present study the acrylation of 1-octanol catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was shown to be as much as an order of magnitude faster when ethyl acrylate served as the donor of the acrylic group. Water activity is a key parameter for optimizing the rate of ester synthesis. The optimum water activity for the esterification of octanol by acrylic acid was found to be 0.75, that for its esterification by propionic acid to be 0.45 and the transesterification involving ethyl acrylate to be fastest at a water activity of 0.3. The reasons for these differences in optimum water activity are discussed in terms of enzyme specificity, substrate solvation, and mass transfer effects.

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Section: Water Activity Dependence With Different Acyl Donorscontrasting

confidence: 58%

Section: Water Activity Dependence With Different Acyl Donorsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 98%

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Efficient enzymatic acrylation through transesterification at controlled water activity

Nordblad¹,

Adlercreutz²

2007

Biotech & Bioengineering

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“…For N435 there is a discrepancy in the sample after 72 h between the acrylation and the esterification reaction-there is a clear activation in the acrylation which cannot be seen in the esterification. It is known that CalB preparations such as N435 have an increasing activity with decreasing water activity (Petersson et al, 2007). One plausible explanation for the initial activation of the preparations from 6 to 72 h could therefore be that water is stripped from the preparations by the reaction mixture.…”

Section: Enzyme Stability In Acrylation Reactionmentioning

confidence: 99%

Biocatalytic polyester acrylation—process optimization and enzyme stability

Hagström

Nordblad

Adlercreutz

2008

Biotech & Bioengineering

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An OH-functional polyester has been acrylated via transesterification of ethyl acrylate, catalyzed by Candida antarctica lipase B (CalB) in two different preparations: Novozym 435 and immobilized on Accurel MP1000. The batch process resulted in incomplete acrylation as well as severe degradation of the polyester. A high degree of acrylation was achieved by optimization through the application of low pressure (15 kPa), continuous inflow of ethyl acrylate and continuous distillation to evaporate the by-product, ethanol. The enzyme preparations displayed good stability with half-lives of 180 and 324 h for Novozym 435 and CalB/MP1000, respectively. This translates into product yields of 3600 and 6200 times the weight of the catalyst, indicating that the enzyme will have a marginal impact on the total process cost.

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“…However, too much water can cause hydrolysis of the TAG [14]. The activity of lipases at diferent water activities is dependent on the source of the enzyme and the type of solvent and immobilization support used [103]. Lipases from molds have shown to be more tolerant to low water activity than bacterial lipases.…”

Section: Inluence Of Water Activitymentioning

confidence: 99%

Synthesis and Characterization of Phenolic Lipids

Roby¹

2017

Phenolic Compounds - Natural Sources, Importance and Applications

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Omega-3 polyunsaturated faty acids (ω3 PUFAs) from ish oils promote well-established health and antiaging beneits that justify their use as functional ingredients in dietary supplements, healthy foods, and nutraceutical products. Dietary supply is needed because human metabolism exhibits limited to synthesize ω3 PUFAs. However, the practical use of such lipids as food ingredients is often limited due to their high susceptibility to oxidation, which is responsible for the undesirable of-lavor and odor of rancid oils, associated with the loss of nutritional value. Produced phenolic lipids were a good solution for all these problems. These phenolic lipids are preferentially produced by enzymatic bioprocesses that exhibit high selectivity toward polyfunctional substrates and mild reaction conditions compared with chemical synthesis pathways. This chapter presents the acylation of phenolic compounds and lipids using enzyme under various operating conditions. In conclusion, the acylation of lipids with natural phenolic compounds resulted in the formation of a lipophilic ester that should be able to stabilize the oil, fats, and emulsions against oxidation. Acylation of lipids to phenolic compounds that have antioxidant properties thus protects the lipids from oxidation, and the phenolic lipid derivatives carry the combined health beneicial properties of lipids and the phenolic molecules.

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A water activity control system for enzymatic reactions in organic media

Cited by 44 publications

References 40 publications

Efficient enzymatic acrylation through transesterification at controlled water activity

Efficient enzymatic acrylation through transesterification at controlled water activity

Biocatalytic polyester acrylation—process optimization and enzyme stability

Synthesis and Characterization of Phenolic Lipids

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