A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells

Song, Lei; Santos‐Sacchi, Joseph

doi:10.1007/978-1-4939-3615-1_28

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…Biol. 2016 Song and Santos-Sacchi (Song & Santos-Sacchi, 2016). WT C57BL/6J and βV spectrin KO littermates at postnatal day 13 and at 2-month-old were compared.…”

Section: Methodsmentioning

confidence: 99%

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Outer Hair Cell Function is Normal in βV Spectrin Knockout Mice

Stankewich

Bai

Stabach

et al. 2021

Preprint

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Reports have proposed a putative role for βV spectrin in outer hair cells (OHCs) of the cochlea. In an ongoing investigation of the role of the cytoskeleton in electromotility, we tested mice with a targeted exon deletion of βV spectrin (Spnb5), and unexpectedly find that Spnb5(-/-) animals’ auditory thresholds are unaffected. Similarly, these mice have normal OHC electromechanical activity (otoacoustic emissions) and non-linear capacitance. Moreover, Spnb5 mRNA is undetectable in the organ of Corti or OHCs. In contrast, magnitudes of auditory brainstem response (ABR) peak 1-amplitudes are significantly reduced. Evidence of a synaptopathy was absent with normal hair cell CtBP-2 counts. In Spnb5(-/-) mice, the number of afferent and efferent nerve fibers is decreased. Taken together, these data establish that βV spectrin is important for hearing, affecting neuronal structure and function. Significantly, these data exclude βV spectrin as functionally important to OHCs as has been previously suggested.

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“…Biol. 2016 Song and Santos-Sacchi (Song & Santos-Sacchi, 2016). WT C57BL/6J and βV spectrin KO littermates at postnatal day 13 and at 2-month-old were compared.…”

Section: Methodsmentioning

confidence: 99%

“…Nonlinear capacitance was conducted in a fashion much like that previously described (Song & Santos-Sacchi, 2016). C57BL/6J WT and βV spectrin KO littermates at postnatal day 13 and at 2 months of age were compared.…”

Section: Methodsmentioning

confidence: 99%

Outer Hair Cell Function is Normal in βV Spectrin Knockout Mice

Stankewich

Bai

Stabach

et al. 2021

Preprint

Self Cite

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“…Nonlinear capacitance was conducted in a fashion much like that previously described (Song & Santos-Sacchi, 2016)…”

Section: Nonlinear Capacitance (Nlc)mentioning

confidence: 99%

Outer hair cell function is normal in βV spectrin knockout mice

Stankewich

Bai²,

Stabach³

et al. 2022

Hearing Research

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“…Because OHC somatic motility, but not hair bundle motility, is the basis of the cochlear amplifier, and because somatic motility is another major source contributing to DPOAE, we further sought to determine the OHC electromechanical transduction status upon Atg7 conditional knockout 28 . We evaluated the voltagedependent charge movement by NLC as a suitable surrogate 29 . As early as P30, typical Atg7-deficient OHCs exhibited reduced NLC/C lin and Q max /C lin , a V h shifted toward a more positive potential, and an increased zvalue.…”

Section: Atg7 Is Essential For the Physiological Function Of Ohcsmentioning

confidence: 99%

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice

Zhou

Qian

et al. 2020

Cell Death Dis

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Atg7 is an indispensable factor that plays a role in canonical nonselective autophagy. Here we show that genetic ablation of Atg7 in outer hair cells (OHCs) in mice caused stereocilium damage, somatic electromotility disturbances, and presynaptic ribbon degeneration over time, which led to the gradual wholesale loss of OHCs and subsequent early-onset profound hearing loss. Impaired autophagy disrupted OHC mitochondrial function and triggered the accumulation of dysfunctional mitochondria that would otherwise be eliminated in a timely manner. Atg7-independent autophagy/mitophagy processes could not compensate for Atg7 deficiency and failed to rescue the terminally differentiated, non-proliferating OHCs. Our results show that OHCs orchestrate intricate nonselective and selective autophagic/mitophagy pathways working in concert to maintain cellular homeostasis. Overall, our results demonstrate that Atg7-dependent autophagy plays a pivotal cytoprotective role in preserving OHCs and maintaining hearing function.

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A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells

Cited by 4 publications

References 36 publications

Outer Hair Cell Function is Normal in βV Spectrin Knockout Mice

Outer Hair Cell Function is Normal in βV Spectrin Knockout Mice

Outer hair cell function is normal in βV spectrin knockout mice

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice

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