A walk on the wild side: Wild ungulates as potential reservoirs of multi-drug resistant bacteria and genes, including Escherichia coli harbouring CTX-M beta-lactamases

Torres, Rita Tinoco; Cunha, Mónica V.; Araújo, Débora América Frezza Villar de; Ferreira, Helena; Fonseca, Carlos; Palmeira, Josman Dantas

doi:10.1016/j.envpol.2022.119367

Cited by 11 publications

(7 citation statements)

References 61 publications

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“…Subsequently, the number of related studies steadily increased, with a notable acceleration between 2016 and 2020, during which approximately 60% of these studies were published [23]. The CTX-M1 group identified in this study exhibited the highest prevalence among free-ranging bantengs, consistent with previous studies by Homeier-Bachmann et al [24], Torres et al [26], Formenti et al [27], Sasaki et al [28] and Niumsup et al [29] on other wild ungulates and healthy individuals in rural Thailand regions. The prevalence of CTX-M1 and CTX-M9 observed in this study is consistent with the findings reported in other studies involving domesticated animals [30,31] and humans [28,29] in South-east Asia.…”

Section: Discussionsupporting

confidence: 87%

Epidemiological study of antimicrobial-resistant bacteria in healthy free-ranging bantengs (Bos javanicus) and domestic cattle

Konputtar,

Yossapol,

Phaechaiyaphum

et al. 2023

Vet World

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Background and Aim: Antimicrobial-resistant microorganisms (ARMs) have been increasing among wild animals. Interactions occurring at the interface between wildlife, humans, and livestock can lead to the transmission of ARMs. Thus, the prevalence of ARMs in wild and domestic animals should be determined to address and prevent this issue. This study aimed to determine the resistance patterns of cefotaxime (CTX)-resistant Escherichia coli and identify the presence of extended-spectrum beta-lactamase (ESBL) genes in ESBL-producing E. coli among a population of wild banteng (Bos javanicus) and domestic cattle kept on farms located close to the Lam Pao non-hunting area, Kalasin province, Thailand. Materials and Methods: Forty-five fecal samples were taken from wild bantengs inhabiting the Lam Pao non-hunting area in Thailand, alongside 15 samples from domestic cattle. Bacterial culture, triple sugar iron, and motile indole lysine tests were conducted to identify E. coli. A polymerase chain reaction (PCR) was conducted for specific confirmation. MacConkey agar supplemented with 2 μg/mL of CTX was used to identify CTX-resistant E. coli, which would be used to identify ESBL production based on a double-disk synergy test. Extended-spectrum beta-lactamase-producing samples were subjected to disk diffusion tests to determine resistant patterns, and the sizes of PCR bands and DNA sequencing were used to differentiate ESBL gene types. Results: All samples tested positive for E. coli. Forty-five isolates from 15 banteng samples and three isolates from one domestic cattle sample displayed CTX-resistant and ESBL-producing traits. The banteng and domestic cattle populations exhibited nine and three distinct resistant patterns, respectively. The PCR results indicated that the banteng isolates harbored the following genes: Cefotaxime-M1 (n = 38), CTX-M9 (n = 5), and the SHV group (n = 2). All three isolates from the domestic cattle sample contained the CTX-M1 gene. Classification of ESBL genes based on the DNA sequences of the banteng isolates showed the characteristics of CTX-M15 (n = 20), CTX-M55 (n = 6), CTX-M14 (n = 5), and CTX-M79 (n = 1). The three domestic cattle isolates exhibited the characteristics of CTX-M15, CTX-M55, and CTX-M79. Conclusion: Despite no previous antibiotic applications, approximately one-third of the banteng samples displayed CTX resistance, indicating ARM contamination within the ecosystem. The similarity in ESBL genes between the banteng and domestic cattle populations suggests potential gene transmissions between these animal groups. However, the initial source of ARMs remains unclear, as the banteng population exhibited more ESBL genes than the domestic cattle, suggesting the possibility of multiple ARM sources. These findings raise concerns because the banteng population inhabits an area that is an important source of freshwater and nourishes the entire north-east region of Thailand and other South-east Asian countries, including Laos, Cambodia, and Southern Vietnam. Keywords: antimicrobial resistance, banteng, cattle, extended-spectrum beta-lactamase, human-wildlife interface, wildlife.

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Section: Discussionsupporting

confidence: 87%

Epidemiological study of antimicrobial-resistant bacteria in healthy free-ranging bantengs (Bos javanicus) and domestic cattle

Konputtar,

Yossapol,

Phaechaiyaphum

et al. 2023

Vet World

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“…Moreover, there have been a growing number of global reports on bla CTX-M-55 . This study revealed that the detection rate of bla CTX-M-55 in waterfowl-derived E. coli was considerably higher than that of other subtypes, marking the earlier reports of such high prevalence ( Kiratisin et al, 2007 ; Hopkins et al, 2008 ; Sun et al, 2010 ; Ma et al, 2012 ; Torres et al, 2022 ). This situation indicates that the high detection rate of bla CTX-M-55 in China's waterfowl breeding industry is not coincidental, which suggests that this genotype may even become the dominant subtype within the bla CTX-M family in this field.…”

Section: Resultssupporting

confidence: 78%

“…In this study, bla CTX-M-65 had the second-highest detection rate. Previous studies have reported that bla CTX-M-15 or bla CTX-M-14 were the most commonly detected subtypes among the bla CTX-M family ( Woodford et al, 2004 ; Damjanova et al, 2008 ; Zhanel et al, 2010 ; Sultan et al, 2022 ; Torres et al, 2022 ). However, in the present study, bla CTX-M-15 was only carried by E. coli LAY43, while bla CTX-M-14 was found in 4 E. coli strains.…”

Section: Resultsmentioning

confidence: 99%

Implications of different waterfowl farming on cephalosporin resistance: Investigating the role of blaCTX-M-55

et al. 2023

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“…According to a study conducted in Switzerland, of the 84 red deer and 64 roe deer tested, ESBL-producing E. coli ( bla CTX-M-1 ) were isolated from only one roe deer [ 26 ]. bla CTX-M-14 - and bla CTX-M-15 -producing E. coli were isolated from red deer (1/62, 1.6%) and fallow deer (1/29, 3.4%) [ 29 ]. ESBL-producing E. coli was also isolated from roe deer (13/573, 2.3%) [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

A survey of antimicrobial-resistant <i>Escherichia coli</i> prevalence in wild mammals in Japan using antimicrobial-containing media

Asai

Usui

Sugiyama

et al. 2022

The Journal of Veterinary Medical Science

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The emergence and spread of antimicrobial-resistant bacteria and resistance genes pose serious human and animal health concerns. Therefore, to control antimicrobial-resistant bacteria in the environment, the status of antimicrobial resistance of Escherichia coli in a variety of wild mammals and their prevalence were examined using antimicrobial-containing media. In total, 750 isolates were obtained from 274/366 (74.9%) wild mammals, and antimicrobial-resistant E. coli was detected in 37/750 isolates (4.9%) from 7 animal species (26/366 [7.1%] individuals). Using antimicrobial-containing media, 14 cefotaxime (CTX)-and 35 nalidixic acid-resistant isolates were obtained from 5 (1.4%) and 17 (4.6%) individuals, respectively. CTX-resistant isolates carried blaCTX-M-27, blaCTX-M-55, blaCTX-M-1, and blaCMY-2, with multiple resistance genes. Fluoroquinolone-resistant isolates had multiple mutations in the quinolone-resistance determining regions of gyrA and parC or qnrB19. Most resistant isolates exhibited resistance to multiple antimicrobials. The prevalence of antimicrobial-resistant bacteria observed in wild mammals was low; however, it is essential to elucidate the causative factors related to the low prevalence and transmission route of antimicrobial-resistant bacteria/resistance genes released from human activities to wild animals and prevent an increase in their frequency.

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A walk on the wild side: Wild ungulates as potential reservoirs of multi-drug resistant bacteria and genes, including Escherichia coli harbouring CTX-M beta-lactamases

Cited by 11 publications

References 61 publications

Epidemiological study of antimicrobial-resistant bacteria in healthy free-ranging bantengs (Bos javanicus) and domestic cattle

Epidemiological study of antimicrobial-resistant bacteria in healthy free-ranging bantengs (Bos javanicus) and domestic cattle

Implications of different waterfowl farming on cephalosporin resistance: Investigating the role of blaCTX-M-55

A survey of antimicrobial-resistant <i>Escherichia coli</i> prevalence in wild mammals in Japan using antimicrobial-containing media

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