A visual atlas of meiotic protein dynamics in living fission yeast

Abstract

Meiosis is a carefully choreographed dynamic process that re-purposes proteins from somatic/vegetative cell division, as well as meiosis-specific factors, to carry out the differentiation and recombination pathway common to sexually reproducing eukaryotes. Studies of individual proteins from a variety of different experimental protocols can make it difficult to compare details between them. Using a consistent protocol in otherwise wild-type fission yeast cells, this report provides an atlas of dynamic protein behaviour of representative proteins at different stages during normal zygotic meiosis in fission yeast. This establishes common landmarks to facilitate comparison of different proteins and shows that initiation of S phase likely occurs prior to nuclear fusion/karyogamy.

Dynamic configurations of meiotic DNA-break hotspot determinant proteins

“…2C, Fig. 4; Table S3), consistent with results of a recent publication that included Rec27 (Escorcia et al, 2021). These results support and extend LinEs being involved in the meiotic chromosomal structural transitions reported in electron microscopy studies of nuclear spreads.…”

“…The time-lapse data of live zygotic meiosis unambiguously showed the LinE structural transitions (Fig. 5; Escorcia et al, 2021;Ding et al, 2021). Moreover, we found that several recombination-deficient LinE mutants form only dotty LinE structures, not the fully established linear forms in wild type (Fig.…”