A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max

Kandoth, Pramod Kaitheri; Heinz, Robert; Yeckel, Gregory; Gross, Nathan W.; Juvale, Parijat S.; Hill, John H.; Whitham, Steven A.; Baum, Thomas J.; Mitchum, Melissa G.

doi:10.1186/1756-0500-6-255

Cited by 30 publications

(26 citation statements)

References 22 publications

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“…BPMV VIGS has also been effective in screens of genes required for resistance to SCN in roots (Liu et al 2012;Kandoth et al 2013). The SCN resistance gene Rhg4 was mapped to a genetic interval containing two genes that were not similar to previously identified plant disease resistance genes (Liu et al 2012).…”

Section: Scnmentioning

confidence: 99%

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Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

Whitham

Eggenberger

Zhang

et al. 2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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Section: Scnmentioning

confidence: 99%

“…A very important consideration for these studies was the establishment of appropriate conditions under which nematode loss-of-resistance phenotypes could be identified reproducibly. In light of this, specific protocols were developed for VIGS of genes involved in soybean-SCN interactions in roots (Kandoth et al 2013). …”

Section: Scnmentioning

confidence: 99%

Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

Whitham

Eggenberger

Zhang

et al. 2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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“…So the knowledge of effector proteins from nematodes could point out many candidate genes for nematode resistance in crop plants. A recently developed virus-induced gene silencing (VIGS) method provides a new tool to identify putative genes involved in soybean-nematode interactions (Kandoth et al, 2013). This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Plant-nematode Interactions: From Genomics to Metabolomics

Ali¹,

Abbas²,

Azeem³

et al. 2015

IJAB

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Plant parasitic nematodes are obligate parasites causing serious reduction in crop yields. Several economically important species parasitize various plant species, but the root knot and cyst nematodes belonging to the Heteroderidae family are especially dangerous. Plant parasitic nematodes result in crop losses of over $150 billion worldwide. This review gives an account of morpho-physiological and molecular events during parasitism of root-knot and cyst nematodes. It describes the transcriptomes and parasitomes of various nematodes indicating that the effector proteins are crucial for the compatible plant nematode interactions. Various sequencing techniques used in plant-nematode genomics and transcriptomics are discussed. Moreover, the dynamics of host transcriptomes in response to infection with different nematode species have been reported. The host transcriptomes have unrevealed many candidate genes, which are involved in both compatible and incompatible plant nematode interactions. The strategy of manipulation of expression of the genes induced and suppressed by the nematodes in the feeding sites has also been suggested for enhancement of resistance against nematodes. This review will provide the researchers with the information regarding transcriptional changes in the nematodes as well as host plants, which would be important for the induction of resistance against nematodes in different crop plants.

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“…glycinea Whitham et al 2016b). In addition, the BPMV system can be used to silence genes in the roots (Juvale et al 2012), and protocols have been developed that enable identification of genes involved in soybean-SCN interactions (Liu et al 2012;Kandoth et al 2013). With regard to abiotic stresses, the BPMV system has been used to assess functions of genes in iron-deficiency chlorosis and salt stress (Rao et al 2013;Atwood et al 2014).…”

Section: Virus-induced Gene Silencing (Vigs)mentioning

confidence: 99%

Soybean Functional Genomics: Bridging the Genotype-to-Phenotype Gap

O’Rourke

Graham

Whitham

2017

Compendium of Plant Genomes

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Technological advances coupled with the economic importance of soybean have led to increased efforts to understand gene function and associate genes with phenotypes of agronomic and fundamental interest. Functional genomics approaches aim to develop sufficient understanding needed to bridge the genotype-tophenotype gap. In general terms, functional genomics approaches begin by using highly parallelized methods to analyze genomes, transcriptomes, proteomes, and metabolomes to generate hypotheses about genes that control phenotypes. Candidate genes are then tested for their contributions to phenotypes through various methods such as RNA silencing, genetic mutation, or overexpression. In this chapter, we review the current approaches, tools, and resources that are being applied for functional genomics research in soybean. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Technological advances coupled with the economic importance of soybean have led to increased efforts to understand gene function and associate genes with phenotypes of agronomic and fundamental interest. Functional genomics approaches aim to develop sufficient understanding needed to bridge the genotype-to-phenotype gap. In general terms, functional genomics approaches begin by using highly parallelized methods to analyze genomes, transcriptomes, proteomes, and metabolomes to generate hypotheses about genes that control phenotypes. Candidate genes are then tested for their contributions to phenotypes through various methods such as RNA silencing, genetic mutation, or overexpression. In this chapter, we review the current approaches, tools, and resources that are being applied for functional genomics research in soybean.

show abstract

A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max

Cited by 30 publications

References 22 publications

Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

Plant-nematode Interactions: From Genomics to Metabolomics

Soybean Functional Genomics: Bridging the Genotype-to-Phenotype Gap

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