A viable foal obtained by equine somatic cell nuclear transfer using oocytes recovered from immature follicles of live mares

Choi, Young-Ho; Norris, Jody D.; Velez, I. C.; Jacobson, Candace C.; Hartman, David L.; Hinrichs, Katrin

doi:10.1016/j.theriogenology.2012.12.005

Cited by 20 publications

(12 citation statements)

References 29 publications

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“…This rate compares favorably with blastocyst rates achieved in our previous studies using 24 h maturation and a 2-h delay from reconstruction to activation, using a similar activation protocol (0-12.5%; Choi et al, 2013a;Choi et al, 2014). The live birth rate from MII ooplasts in the 5-to 8-h R-A treatments (4/17, 24%) is similar to that previously achieved in our laboratory using 24 h of maturation and a 2-h R-A delay, with a similar activation protocol (17-50%; Choi et al, 2009;Choi et al, 2013a;Hinrichs et al, 2007). The optimal R-A delay varies among species (Choi et al, 2004;Liu et al, 2013;Wakayama et al, 1998).…”

Section: Discussionsupporting

confidence: 79%

“…S everal articles have been published reporting production of cloned foals (Choi et al, 2009;Choi et al, 2013a;Choi et al, 2014;Galli et al, 2003;Gambini et al, 2012;Hinrichs et al, 2006;Hinrichs et al, 2007;Lagutina et al, 2005). In these reports, the rates of blastocyst production were low, typically < 10%.…”

Section: Introductionmentioning

confidence: 99%

“…Collection of oocytes from live mares by transvaginal ultrasound-guided aspiration (TVA; Jacobson et al 2010) is an alternative method for obtaining equine oocytes. Production of cloned foals using immature oocytes recovered by TVA is possible (Choi et al, 2013a;Choi et al, 2014), but because of the low number of oocytes obtained per aspiration and the low blastocyst rate, it is laborintensive, time-consuming, and costly. To support continued work in equine NT, research is needed on factors affecting the efficiency of blastocyst production.…”

Section: Introductionmentioning

confidence: 99%

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Timing Factors Affecting Blastocyst Development in Equine Somatic Cell Nuclear Transfer

ChoiYoung-Ho

VelezIsabel

Macías-GarcíaBeatriz

et al. 2015

Cellular Reprogramming

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In nuclear transfer (NT), exposure of donor cell chromatin to the ooplast cytoplasm may aid reprogramming; however, the length of exposure feasible is limited by the developmental life span of the oocyte. We examined the effect of duration of nucleus-cytoplasmic exposure before activation and of in vitro maturation (IVM) in equine NT. In experiment 1, 24 h IVM and a delay of 2, 5, or 8 h between reconstruction and activation yielded 4%, 15%, and 11% blastocysts, respectively. In experiment 2, a 5-h activation delay yielded 17% and 22% blastocysts with two donor cell lines. In experiment 3, using a 5-h activation delay, the blastocyst rate was significantly higher using oocytes after 20 h IVM than after 24 h IVM; however, only 28% of oocytes were in metaphase II (MII) at 20 h. In experiment 4, oocytes were denuded of cumulus at 20 h, and those in metaphase I (MI) were returned to culture for 3 h (20+3H treatment); blastocyst rates were 30% and 27%, respectively (8-h and 5-h delay to activation, respectively). Four live foals resulted from the transfer of 17 blastocysts (24%) produced using MII oocytes and a 5- or 8-h activation delay. Use of equine oocytes immediately after reaching MII, combined with a longer delay from reconstruction to activation, increased developmental competence after equine NT.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Timing Factors Affecting Blastocyst Development in Equine Somatic Cell Nuclear Transfer

ChoiYoung-Ho

VelezIsabel

Macías-GarcíaBeatriz

et al. 2015

Cellular Reprogramming

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“…The development of this technique in equines has several interests (Choi et al, ; Hinrichs , ; Smits, Hoogewijs, Woelders, Daels, & Van Soom, ; Vanderwall et al, , ) genetic preservation of some endangered species and breeds; genetic preservation of high value animals that can no longer breed, such as geldings; and replacement of pets for sentimental and emotional purposes. The preservation of high genetic value animals is the first application of cloning currently (Choi et al, ; Vanderwall et al, ). The development of this technique was done gradually, and distribution of cloning varies over the world.…”

Section: Introductionmentioning

confidence: 99%

“…The first time that the reproductive capacity of a cloned mare was tested was in 2008 when Prometea gave birth to a healthy foal, demonstrating the fertility and fecundity of cloned mares. (Choi et al, ).…”

Section: Introductionmentioning

confidence: 99%

Assessing the fertility of two mares cloned from the same founder animal

Dordas-Perpinyà

Pintart

Terris³

et al. 2019

Reprod Domestic Animals

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Two cloned mares, produced from the same sample of skin fibroblasts, were bred during four breeding seasons from their second year of age, as embryo donors, in exactly the same conditions, using the same stallions for both cloned mares. The aim of this study was to test the embryo donor potential of cloned mares and to compare the results obtained from two cloned mares of the same mare with other embryo donor mares (n = 31–39 per breeding season) at the same stud. For both cloned mares, 19 embryos were recovered by 43 collection attempts (44%) (7/22 for one; 12/21 for the other), 16 (84%) pregnancies (5/7 for one, 11/12 for the other) were obtained at day 14 post‐ovulation (D14), and 12 (3/7 for one; 9/12 for the other) foals were born. One cloned mare was a less efficient donor mare than the other (p < .05), In control donor mares, 623 embryo collections were performed, with a recovery rate (80%—496/623) significantly higher than for cloned mares. The recovery rate in the subpopulation of 2–5‐year‐old control donor mares (same age of cloned mares) (89%—127/143) and The recovery rate in the subpopulation of 12 control mares bred with the seven same stallions as clones (55%—17/31), were both higher than for cloned mare (p < .05). The success rate of transfer was not different between embryos produced by cloned mares (84%—16/19) and those produced by control donor mares (79%—392/496). However, the foaling rate per embryo collection was significantly lower for cloned mares (28%—12/43) than for control donor mares (52% ‐ 325/623) (p < .05).

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Horse Somatic Cell Nuclear Transfer Using Zona Pellucida-Enclosed and Zona-Free Oocytes

Salamone

Maserati

2023

Methods in Molecular Biology

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A viable foal obtained by equine somatic cell nuclear transfer using oocytes recovered from immature follicles of live mares

Cited by 20 publications

References 29 publications

Timing Factors Affecting Blastocyst Development in Equine Somatic Cell Nuclear Transfer

Timing Factors Affecting Blastocyst Development in Equine Somatic Cell Nuclear Transfer

Assessing the fertility of two mares cloned from the same founder animal

Horse Somatic Cell Nuclear Transfer Using Zona Pellucida-Enclosed and Zona-Free Oocytes

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