A very small porcine virus with circular single-stranded DNA

Tischer, Ilse; Gelderblom, Hans R.; Vettermann, W.; Koch, Meinrad A.

doi:10.1038/295064a0

Cited by 662 publications

(479 citation statements)

References 8 publications

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“…PCV2 é o menor vírus animal descrito com capacidade de replicação autônoma em células de mamíferos, que ocorre, obrigatoriamente, durante a fase S da mitose, pois esse agente biológico depende de proteínas produzidas durante essa fase do ciclo celular. Essa característica deve ser considerada quando se pretende trabalhar com o isolamento, e a D-glucosamina é amplamente utilizada para induzir a fase S nas células, sendo recomendados a concentração de 300 mM e o período de incubação de 30 minutos (TISCHER et al, 1982). Porém, em razão da natureza tóxica desse reagente, observa-se severa degeneração nas células infectadas e tratadas com D-glucosamina quando comparadas com as células infectadas e não tratadas (STEVENSON et al, 1999).…”

Section: Introductionunclassified

Circovírus suíno 2b: isolamento e taxa de mutação em linhagem de célula de macrófagos (J744)

et al. 2017

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RESUMO: Porcine circovirus 2 (PCV2) está associado a vários sinais clínicos que são designados coletivamente como Circovirose e tem grande impacto na suinocultura. O isolamento viral é classicamente realizado em células da linhagem PK-15, contudo outras células têm sido testadas. Apesar dos avanços nos estudos com PCV2, o isolamento ainda é um desafio. Diante da dificuldade de manutenção dessas linhagens celulares comumente utilizadas associadas à necessidade do uso de substâncias tóxicas para o isolamento de PCV2, os objetivos do presente trabalho foram descrever o primeiro isolamento de Porcine circovirus 2b em linhagens de células de macrófago (J744) e verificar a taxa de mutação nesse sistema. Uma amostra de pulmão foi submetida ao sequenciamento e agrupada ao genótipo PCV2b. Essa amostra foi utilizada para inocular uma garrafa de J744 (com 30% de confluência em meio RPMI com 10% de soro fetal bovino) e submetida a cinco passagens, as quais foram acompanhadas por reação em cadeia da polimerase quantitativa (PCRq). As cargas virais inicial e final foram de 2,90 × 103 e de 4,45 × 108 cópias de DNA/µL para PCV2b, respectivamente. O sequenciamento confirmou o isolamento e descartou o coisolamento de mais de um genótipo. Após cinco passagens, o isolado apresentou identidade de 99,7%, com descrição de cinco mutações pontuais, uma sinônima e quatro não sinônimas, observadas nas regiões do gene cap e rep. Os resultados obtidos demonstram que as células J744 apresentam a susceptibilidade, e a instabilidade do vírus em J744 será importante para a compreensão do vírus.

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Section: Introductionunclassified

Circovírus suíno 2b: isolamento e taxa de mutação em linhagem de célula de macrófagos (J744)

et al. 2017

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“…Porcine circovirus 2 (PCV2; Circovirus, Circoviridae; Hamel et al, 1998) is a small, icosahedral, nonenveloped virus that contains a single-stranded circular DNA genome of 1.76 kb (Tischer et al, 1982). This virus is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in domestic pigs (Allan et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Detection and Genetic Characterization of Porcine Circovirus 2 Isolates From the First Cases of Postweaning Multisystemic and Wasting Syndrome in Wild Boars in Greece

Sofia

Billinis

Psychas

et al. 2008

Journal of Wildlife Diseases

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ABSTRACT:In 2002, postweaning multisystemic wasting syndrome (PMWS) was diagnosed in a European female wild boar (Sus scrofa), based on the detection of porcine circovirus 2 (PCV2) DNA in various organs, including the uterus, and on histopathologic lesions. This is the first detection of PCV2 DNA in the uterus of a wild boar. Three years later (2005), a wild boar ,6-8 mo of age was found moribund. It presented wasting and dyspnea and finally died. PCV2 DNA was detected in tissue samples, and histopathologic lesions consistent with PMWS were observed. Both wild boars were from neighboring hunting areas in central Greece. Two PCV2 strains from the wild boars were genetically characterized and compared to other reported PCV2 sequences from wild boars and domestic pigs. The PCV-2 sequences from the wild boars in this study were closely related to each other and were grouped with two isolates from wild boars from Hungary. The phylogenetic analysis revealed that the virus might be transmitted between hunting areas. In addition, PCV2 may spread from domestic pigs to wild boars and vice versa.

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“…The virions are spherical, non-enveloped with an icosahedral symmetry. The genome consists of a singlestranded molecule of circular DNA [18,25]. PCV2 is recognised as the cause of a clinical condition of swine named postweaning multisystemic wasting syndrome (PMWS) [2,7,9].…”

Section: Introductionmentioning

confidence: 99%

Lack of an effect of a commercial vaccine adjuvant on the development of postweaning multisystemic wasting syndrome (PMWS) in porcine circovirus type�2 (PCV2) experimentally infected conventional pigs

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Segalés

Balasch³

et al. 2004

Vet. Res.

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-The objective of this study was to evaluate the effect of a commercial vaccine adjuvant on the clinical and pathological outcome of PCV2 experimentally infected 8 to 9-week-old conventional pigs. Forty-four pigs were divided into four groups: non-infected control pigs, pigs that received a vaccine adjuvant, pigs inoculated with PCV2, and pigs inoculated with PCV2 together with the vaccine adjuvant. Infection was monitored until 69 days post-inoculation (PI). Some PCV2 inoculated pigs had hyperthermia, but no other clinical signs were recorded. No characteristic PMWS gross or microscopic lesions were observed in any of the pigs. PCV2 DNA was detected in lymphoid tissues by in situ hybridisation in 6 PCV2 inoculated pigs on day 69 PI. All PCV2 inoculated pigs seroconverted between days 21 and 49 PI, shortly after viremia detection. Moreover, viremia was detected between days 7 and 69 PI using PCR. A peak of the virus load was detected by real-time quantitative PCR between days 14 and 21 PI. There were no significant differences in the proportion of PCV2 positive serum and in the viral load between PCV2 and PCV2 + adjuvant inoculated pigs. Although PMWS was not reproduced in neither PCV2 nor PCV2 + adjuvant inoculated pigs, viremia detection and seroconversion indicated that all PCV2 inoculated pigs developed a chronic long-term asymptomatic infection. An increase of PCV2 replication was not observed in pigs inoculated with the adjuvant. These results indicate that the principle of immunostimulation may not be applicable under the experimental conditions used, suggesting that not all adjuvants used in commercial vaccines are capable of triggering mechanisms for PMWS development.

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A very small porcine virus with circular single-stranded DNA

Cited by 662 publications

References 8 publications

Circovírus suíno 2b: isolamento e taxa de mutação em linhagem de célula de macrófagos (J744)

Circovírus suíno 2b: isolamento e taxa de mutação em linhagem de célula de macrófagos (J744)

Detection and Genetic Characterization of Porcine Circovirus 2 Isolates From the First Cases of Postweaning Multisystemic and Wasting Syndrome in Wild Boars in Greece

Lack of an effect of a commercial vaccine adjuvant on the development of postweaning multisystemic wasting syndrome (PMWS) in porcine circovirus type�2 (PCV2) experimentally infected conventional pigs

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