A very efficient bioconversion of soybean phytosterols mixtures to androstanes by mycobacteria

Pérez, Celso; Falero, Alina; Duc, H. Luu; Balcinde, Yaisme; Hung, Blanca Rosa

doi:10.1007/s10295-006-0148-6

Cited by 29 publications

(17 citation statements)

References 15 publications

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“…By conducting biocatalytic processes in non-aqueous systems, one can extend catalytic specificity of the enzymes and substrates toward new substrates in novel reactions systems. For instance, substrates such as plant sterols [4], cinnamoylated lipids [5] and commodity vegetable oils [6], which are less soluble in water, could benefit significantly from non-aqueous media in which the substrates dissolve and the reaction occurs at a reasonable rate.…”

Section: Introductionmentioning

confidence: 99%

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Supported Phospholipid Bilayer Interaction with Components Found in Typical Room-Temperature Ionic Liquids – a QCM-D and AFM Study

Evans

2008

IJMS

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Quartz crystal microbalance with dissipation (QCM-D) monitoring and atomic force microscopy (AFM) were combined to evaluate the defects created by an ionic liquid anion and a cation in a supported phospholipid bilayer composed of zwitterionic lipids on a silica surface. The cation 1-octyl-3-methyl imidazolium (OMIM + ) was shown to remove lipids from the bilayer, increase the roughness to approximately 2.8 nm (~0.2 for stable supported bilayer) and possibly redeposit lipids with entrapped water. The anion bis(trifluoromethylsulfonyl)imide (Tf 2 N -) was found to leave distinct defects within the bilayer that had large pore-like interiors which left the surrounding bilayer intact. However, the ionic liquid 1-butyl-1-methyl pyrrolidinium bis(trifluoromethylsulfonyl)imide (BMPTf 2 N) formed a film over the supported bilayer. This work demonstrates, for the first time, the direct effects common components of ionic liquids have on a supported phospholipids bilayer.

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Section: Introductionmentioning

confidence: 99%

“…Typically, bioconversion of substrates like sterols, lipids and vegetable oils is done in organic solvents [4–6] because of the substrates’ incompatibility with water. However, typical organic solvents are hazardous because of their flammability and high vapor pressure.…”

Section: Introductionmentioning

confidence: 99%

Supported Phospholipid Bilayer Interaction with Components Found in Typical Room-Temperature Ionic Liquids – a QCM-D and AFM Study

Evans

2008

IJMS

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“…It is well-known that phytosterols (PSs) are suitable raw materials for microbial degradation to 17-ketosteroids because of low cost and easy availability (Fernandes and Cabral, 2007). In recent years, microbial selective cleavages of PS to AD and ADD have been reported by many researchers (Huang et al, 2006;Pérez et al, 2006;Sripalakit et al, 2006;Wang et al, 2006). All things considered, the major obstacle of microbial transformation of PS is the low aqueous solubility (1 μmol/L) of substrate (Malaviya and Gomes, 2008).…”

Section: Introductionmentioning

confidence: 99%

Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

Zhang

Peng

et al. 2013

J. Zhejiang Univ. Sci. B

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Biotransformation of phytosterol (PS) by a newly isolated mutant Mycobacterium neoaurum ZJUVN-08 to produce androstenedione has been investigated in this paper. The parameters of the biotransformation process were optimized using fractional factorial design and response surface methodology. Androstenedione was the sole product in the fermentation broth catalyzed by the mutant M. neoaurum ZJUVN-08 strain. Results showed that molar ratio of hydroxypropyl-β-cyclodextrin (HP-β-CD) to PS and substrate concentrations were the two most significant factors affecting androstenedione production. By analyzing the statistical model of three-dimensional surface plot, the optimal process conditions were observed at 0.1 g/L inducer, pH 7.0, molar ratio of HP-β-CD to PS 1.92:1, 8.98 g/L PS, and at 120 h of incubation time. Under these conditions, the maximum androstenedione yield was 5.96 g/L and nearly the same with the non-optimized (5.99 g/L), while the maximum PS conversion rate was 94.69% which increased by 10.66% compared with the non-optimized (84.03%). The predicted optimum conditions from the mathematical model were in agreement with the verification experimental results. It is considered that response surface methodology was a powerful and efficient method to optimize the parameters of PS biotransformation process.

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“…These include mycobacteria, which seem perform the best. This is why most of the microbial strains used in industrial and academic research are members of the genus Mycobacterium (Atrat, et al, 1991;Fernandes, et al, 2003;Perez, et al, 2006). However, mycobacteria have two serious deficiencies.…”

Section: Development Of Ideal Organismsmentioning

confidence: 99%

From Soybean Phytosterols to Steroid Hormones

Wang¹,

Yao²,

Wei³

2011

Soybean and Health

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A very efficient bioconversion of soybean phytosterols mixtures to androstanes by mycobacteria

Cited by 29 publications

References 15 publications

Supported Phospholipid Bilayer Interaction with Components Found in Typical Room-Temperature Ionic Liquids – a QCM-D and AFM Study

Supported Phospholipid Bilayer Interaction with Components Found in Typical Room-Temperature Ionic Liquids – a QCM-D and AFM Study

Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08

From Soybean Phytosterols to Steroid Hormones

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