1999
DOI: 10.1159/000031026
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A Very–High–Purity von Willebrand Factor Preparation Containing High–Molecular–Weight Multimers

Abstract: Background and Objectives: We investigated large–scale production of very–high–purity von Willebrand factor (vWf) containing high–molecular–weight vWf multimers, using chromatography alone. Materials and Methods: Factor VIII (FVIII)–containing vWf was produced by initial separation of vWf from plasma by gel filtration followed by two ion exchange steps. Two virus inactivation steps were incorporated in the process. Results: The process resulted in FVIII–containing vWf preparations with a mean specific activity… Show more

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