A versatile technique for patterning biomolecules onto glass coverslips

Lom, Barbara; Healy, Kevin E.; Hockberger, Philip E.

doi:10.1016/0165-0270(93)90044-r

Cited by 138 publications

(74 citation statements)

References 27 publications

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“…Lom et al 116,269 used patterns of aminosilane (N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane [EDS]) and alkylsilane (dimethyldichlorosilane [DMS]) SAMs to spatially control the attachment of osteosarcoma cells; bone-derived cells required the presence of serum or vitronectin to organize in compliance with the lithographically defined surface chemistry. 270 Cells were randomly distributed over the EDS/DMS surface at the time of plating but reorganized on the EDS regions within 30 minutes.…”

Section: Vg Bone Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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Section: Vg Bone Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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“…Upon serum starvation, Rac1-expressing cells or control cells showed abundant focal adhesion structures at the periphery of cells Western blot of N1E-115 cells expressing empty vector, Rac1 or Rac3 that were cultured in 2% serum (lanes 1, 2 and 3). As positive control (Lom et al, 1993), N1E-115 cells were starved during 4 hours and subsequently stimulated with 5 M LPA for 15 min to induce maximal MLC phosphorylation. As a loading control, both ␤-actin and total MLC levels are shown.…”

Section: Rac3-expressing Cells Show Defect Cell-matrix Adhesionsmentioning

confidence: 99%

Rac1 and Rac3 have opposing functions in cell adhesion and differentiation of neuronal cells

Hajdo-Milašinović

Ellenbroek

et al. 2007

Journal of Cell Science

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“…Several methods have been developed for patterning surfaces to control neuronal attachment and growth (Hammarback et al, 1985;Kleinfeld et al, 1988;Corey et al, 1991;Stenger et al, 1992;Matsuda et al, 1992;Lom et al, 1993;Hickman et al, 1994;Corey et al, 1997;Branch et al, 1998;Ravenscroft et al, 1998;Branch et al, 2000;James et al, 2000;Lauer et al, 2001). Using these techniques it is possible to direct neuron cell bodies to electrodes and thus provide the ability to study individual neurons within a neural network.…”

Section: Introductionmentioning

confidence: 99%

Low-density neuronal networks cultured using patterned poly-l-lysine on microelectrode arrays

Jun

Hynd

Dowell-Mesfin

et al. 2007

Journal of Neuroscience Methods

101

100

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Synaptic activity recorded from low-density networks of cultured rat hippocampal neurons was monitored using microelectrode arrays (MEAs). Neuronal networks were patterned with poly-Llysine (PLL) using microcontact printing (µCP). Polydimethysiloxane (PDMS) stamps were fabricated with relief structures resulting in patterns of 2 µm-wide lines for directing process growth and 20 µm-diameter circles for cell soma attachment. These circles were aligned to electrode sites. Different densities of neurons were plated in order to assess the minimal neuron density required for development of an active network. Spontaneous activity was observed at 10-14 days in networks using neuron densities as low as 200 cells/mm 2 . Immunocytochemistry demonstrated the distribution of dendrites along the lines and the location of foci of the presynaptic protein, synaptophysin, on neuron somas and dendrites. Scanning electron microscopy demonstrated that single fluorescent tracks contained multiple processes. Evoked responses of selected portions of the networks were produced by stimulation of specific electrode sites. In addition, the neuronal excitability of the network was increased by the bath application of high K + (10-12 mM). Application of DNQX, an AMPA antagonist, blocked all spontaneous activity, suggesting that the activity is excitatory and mediated through glutamate receptors.

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A versatile technique for patterning biomolecules onto glass coverslips

Cited by 138 publications

References 27 publications

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Rac1 and Rac3 have opposing functions in cell adhesion and differentiation of neuronal cells

Low-density neuronal networks cultured using patterned poly-l-lysine on microelectrode arrays

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