A versatile signal-on electrochemical biosensor for Staphylococcus aureus based on triple-helix molecular switch

Cai, Rongfeng; Zhang, Zhongwen; Chen, Haohan; Tian, Yaping; Zhou, Nandi

doi:10.1016/j.snb.2020.128842

Cited by 68 publications

(46 citation statements)

References 45 publications

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“…In the work performed by Cai and co-workers [ 40 ], the amplification step was performed for 45 min, which is a shorter time compared with the original 2-h SDA process which has been considered too long for the development of biosensors. Moreover, SDA reactions have shown high sensitivity to background DNA, which will coamplify following non-specific primer binding as a result of reduced stringency conditions present at 37 °C.…”

Section: Isothermal Dna Amplification-based Biosensors: State Of Tmentioning

confidence: 99%

“…Moreover, Arabidopsis and tomato plants were analysed by a multiplexed RPA-based SERS platform [ 33 ] for the detection of P. syringae , B. cinerea and F. oxyspurim. Finally, the SDA-based biosensor was applied to the detection of E. coli in spiked lake water, tap water and honey samples [ 40 ]. Some of the mentioned works showed good recovery values [ 19 , 24 , 40 ] and correlated well with results obtained with other techniques such as qPCR [ 28 , 32 ] or PCR and gel electrophoresis [ 20 , 29 , 33 ].…”

Section: Isothermal Dna Amplification-based Biosensors: Key Factormentioning

confidence: 99%

“…The complex asynchronous reactions occur concurrently at 37 °C (after initial heat denaturation for dsDNA targets). Although SDA was first described in 1992 [72], to the best of our knowledge, only one SDA-based biosensor for the detection of S. aureus has been published [40]. In this work, an aptamer is used as a biorecognition molecule.…”

Section: Sda-based Biosensorsmentioning

confidence: 99%

“…This method uses a hairpin DNA probe, a short primer, and a DNA polymerase. While the hairpin loop sequence is complementary to the In the work performed by Cai and co-workers [40], the amplification step was performed for 45 min, which is a shorter time compared with the original 2-h SDA process which has been considered too long for the development of biosensors. Moreover, SDA reactions have shown high sensitivity to background DNA, which will coamplify following non-specific primer binding as a result of reduced stringency conditions present at 37 • C. The use of aptamers provides high selectivity to the assay ascribed to the high specificity of the aptamers for their corresponding targets.…”

Section: Isdpr-based Biosensorsmentioning

confidence: 99%

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Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Leonardo

Toldrà

Campàs

2021

Sensors

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The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the field of food safety and environmental monitoring. Optical and electrochemical biosensors based on loop mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), helicase dependent amplification (HDA), strand displacement amplification (SDA), and isothermal strand displacement polymerisation (ISDPR) are described, and an overview of their current advantages and limitations is provided. Although further efforts are required to harness the potential of these emerging analytical techniques, the coalescence of the different isothermal amplification techniques with the wide variety of biosensing detection strategies provides multiple possibilities for the efficient detection of bacteria far beyond the laboratory bench.

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Section: Isothermal Dna Amplification-based Biosensors: State Of Tmentioning

confidence: 99%

Section: Isothermal Dna Amplification-based Biosensors: Key Factormentioning

confidence: 99%

Section: Sda-based Biosensorsmentioning

confidence: 99%

Section: Isdpr-based Biosensorsmentioning

confidence: 99%

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Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Leonardo

Toldrà

Campàs

2021

Sensors

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“…An electrochemical biosensor for Staphylococcus aureus was designed, based on a triple-helix molecular switch, which can control the switching of electrochemical signals [116]. The biosensor showed a dynamic range from 30 to 3 x 10 8 CFUmL -1 , with a detection limit of 8 CFUmL -1 .…”

Section: Pathogenic Bacteriamentioning

confidence: 99%

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Curulli

2021

Preprint

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Safety and quality are key issues for the food industry. Consequently, there is a growing demand to preserve the food chain and products against substances toxic, harmful to human health such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long time of analyses, expensive and complex procedures, and they require skilled personnel. Therefore, the development of performant electrochemical biosensors can significantly support the screening of food chain and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in the food screening and the challenges to address.

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Conjugation of Carbohydrates with Quinolines: A Powerful Synthetic Tool

Kaufman¹,

Larghi

2022

Eur J Org Chem

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A detailed revision on natural and synthetic glycosylated quinolines, spanning the last 20 years, is presented. Exhaustive descriptions about the state of the art of the synthesis and relevant applications of these glyco‐heterocycles are provided. In addition, an account of the natural products containing this motif and their associated bioactivity data are also outlined. For the sake of consistency, quinolones are also covered as well as hybrid compounds, embodied with linkers between the heterocyclic ring and the carbohydrate fragment.

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A versatile signal-on electrochemical biosensor for Staphylococcus aureus based on triple-helix molecular switch

Cited by 68 publications

References 45 publications

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Conjugation of Carbohydrates with Quinolines: A Powerful Synthetic Tool

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