A versatile real-time PCR method to quantify bovine contamination in buffalo products

Drummond, Marcela Gonçalves; Brasil, B.S.A.F.; Dalsecco, Lissandra Sousa; Brasil, R.S.A.F.; Teixeira, Lílian Viana; Oliveira, Denise Aparecida Andrade de

doi:10.1016/j.foodcont.2012.05.051

Cited by 52 publications

(29 citation statements)

References 33 publications

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“…A positive (cultured isolates C. pecorum E58, C. psittaci CR009 and C. abortus B577 DNA) and negative (MilliQ water) control was included in each run, with all samples tested in duplicate . Bovine mitochondrial DNA ( Cytochrome B) was used as an internal standard to assess the integrity of the sample and the presence and/or absence of PCR inhibitors . In this study, an animal was considered positive for Chlamydia spp., if chlamydial DNA was detected from a single anatomical site, with each sample positive in duplicate, whereas a herd was considered positive if at least one animal was tested positive for Chlamydia spp.…”

Section: Chlamydia Detection Rates In Samples From Dairy Herds and Onmentioning

confidence: 99%

Chlamydial infection and on‐farm risk factors in dairy cattle herds in South East Queensland

2019

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Chlamydial infections in dairy cattle are common and have been sporadically associated with reduced performance and severe disease manifestations. While chlamydial infections are well described in sheep, very little is known about the epidemiology of these infections in dairy cattle in Australia. In this study, we screened for chlamydial infections and assessed on-farm risks in dairy cattle herds from Southeast Queensland (SE Qld) region of Australia.In total, 228 paired vaginal and rectal swabs were collected from 114 visually healthy dairy cows from four farms in SE Qld. Risk factors were rated by observational study and included: hygiene and cleanliness of cows, walkway and parlour, incidence of perinatal mortality, external replacements, mode of breeding, calving pen management, heat reduction strategies, and feed ration usage.Testing for chlamydial pathogens (Chlamydia pecorum, Chlamydia psittaci and Chlamydia abortus) was done using species-specific quantitative polymerase chain reaction (qPCR) assays. Detected rates of chlamydial infection were evaluated against the on-farm risk factors.C. pecorum infection was widespread in all four farms, with 56.1% (64/114) of individual animals shedding this organism from vaginal and rectal, or both sites. C. abortus and C. psittaci were not detected in any animals. No association was found to exist with risk factors and C. pecorum infection rates in our study, however the number of Chlamydia positive animals was statistically different between the herds.This study suggests that subclinical chlamydial infections may impact on dairy herd health at the production level rather than affecting individual animal.

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Section: Chlamydia Detection Rates In Samples From Dairy Herds and Onmentioning

confidence: 99%

Chlamydial infection and on‐farm risk factors in dairy cattle herds in South East Queensland

2019

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“…The method for 10‐species detection developed in this study is based on the principles of species‐specific real‐time PCR, combined with amplification of a CytB gene fragment by universal primers (used here as IPC) and the use of melt curve analysis for amplicon check. Several real‐time PCR methods are available for species detection in meat and meat products, most of them concerning the use of TaqMan probes (Zhang and others ; Cammà and others ; Kesmen and others ; Drummond and others ; Kim and others ). Some methods are based on the DNA intercalating dye SYBR green to detect pork in poultry meat products (Soares and others ) and bovine and poultry in foodstuffs (Safdar and Junejo ).…”

Section: Resultsmentioning

confidence: 99%

“…Buffalo‐specific primers used in this study were described by Drummond and others () and bovine‐specific primers were modified from those described by Zhang and others (; Table ). The species‐specific primers for the remaining 8 species were designed using published sequences from CytB gene (accession numbers presented in Table ), with support from Primer‐BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast) by NCBI (Natl.…”

Section: Methodsmentioning

confidence: 99%

A Fast and Reliable Real‐Time PCR Method for Detection of Ten Animal Species in Meat Products

Dalsecco

Palhares²,

Oliveira³

et al. 2018

Journal of Food Science

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“…A series of methods based on the quantity and nature of the milk proteins in cheese have been developed to detect this type of adulteration . One of the points of interest in evaluating the authenticity of cheeses is to identify single markers or groups of markers that characterise the individuality of the species in the elaboration of the products, and electrophoretic and chromatographic methods stand out amongst the techniques used for this purpose Loparelli et al, 2007;Russo et al, 2012;Drummond et al, 2013;Guerreiro et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the peptide profile with a view to authenticating buffalo mozzarella cheese

Gonçalves

Silva

Pontes

et al. 2016

Int J of Food Sci Tech

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Summary Electrophoretic and chromatographic techniques were used to determine water‐soluble peptide profiles aiming to identify the adulteration of buffalo milk mozzarella cheese by the addition of cow's milk. Thus, cheeses were produced with contents of cow's milk varying from 0% to 100%, and the peptides extracted after production and after 20 days of refrigeration. Polyacrylamide gel electrophoresis under denaturing conditions in the presence of sodium dodecyl sulphate (SDS‐PAGE) identified a potential peptide marker of exclusively bovine origin with a size of about 21 kDa for the addition of cow's milk above 30%. Reverse‐phase high‐performance liquid chromatography (RP‐HPLC) indicated the existence of two potential peptides present in higher concentrations in buffalo milk and one exclusive for cow's milk, the latter making it possible to estimate the addition of cow's milk to buffalo milk. Six commercial brands of buffalo mozzarella cheese were evaluated, and indications of adulteration found in four of them.

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A versatile real-time PCR method to quantify bovine contamination in buffalo products

Cited by 52 publications

References 33 publications

Chlamydial infection and on‐farm risk factors in dairy cattle herds in South East Queensland

Chlamydial infection and on‐farm risk factors in dairy cattle herds in South East Queensland

A Fast and Reliable Real‐Time PCR Method for Detection of Ten Animal Species in Meat Products

Evaluation of the peptide profile with a view to authenticating buffalo mozzarella cheese

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