A versatile method to separate complex lipid mixtures using 1-butanol as eluent in a reverse-phase UHPLC-ESI-MS system

Kok, Niels A. W. de; Exterkate, Marten; Andringa, Ruben L. H.; Minnaard, Adriaan J.; Driessen, Arnold J. M.

doi:10.1016/j.chemphyslip.2021.105125

Cited by 12 publications

(23 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lipidome of the MW001 strain was analyzed during the exponential and stationary phases of growth in the absence and presence of terbinafine ( Figure 3 , Table 1 ). The accumulation of inositol phosphate dialkyl glycerol diether lipid (IP-DGD, m / z 893.685 [M-H] − ) was observed in exponential and stationary phase cells ( Figure 3 and Figure 4 ) [ 15 , 27 ]. IP-DGD was the only diether lipid that showed consistent accumulation across growth phases ( Figure 3 and Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…IP-DGD was the only diether lipid that showed consistent accumulation across growth phases ( Figure 3 and Figure 4 ). The levels of DGGGP ( m / z 715.507 [M-H] − ) and archaetidic acid (AA, m / z 731.632 [M-H] − ) were slightly elevated during the exponential phase; however, they were depleted during the stationary phase ( Figure 3 and Figure 4 ) [ 15 , 27 ]. The lipidome of S. acidocaldarius was examined for GDGTs with various cyclopentane rings (0–8) [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…The levels of DGGGP ( m / z 715.507 [M-H] − ) and archaetidic acid (AA, m / z 731.632 [M-H] − ) were slightly elevated during the exponential phase; however, they were depleted during the stationary phase ( Figure 3 and Figure 4 ) [ 15 , 27 ]. The lipidome of S. acidocaldarius was examined for GDGTs with various cyclopentane rings (0–8) [ 27 ]. A slight decrease in the levels of GDGT-1 to 3 was observed in the exponential phase of growth with terbinafine ( Supplementary Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Membrane Adaptations and Cellular Responses of Sulfolobus acidocaldarius to the Allylamine Terbinafine

Rao

Kok

Driessen

2023

IJMS

Self Cite

View full text Add to dashboard Cite

Cellular membranes are essential for compartmentalization, maintenance of permeability, and fluidity in all three domains of life. Archaea belong to the third domain of life and have a distinct phospholipid composition. Membrane lipids of archaea are ether-linked molecules, specifically bilayer-forming dialkyl glycerol diethers (DGDs) and monolayer-forming glycerol dialkyl glycerol tetraethers (GDGTs). The antifungal allylamine terbinafine has been proposed as an inhibitor of GDGT biosynthesis in archaea based on radiolabel incorporation studies. The exact target(s) and mechanism of action of terbinafine in archaea remain elusive. Sulfolobus acidocaldarius is a strictly aerobic crenarchaeon thriving in a thermoacidophilic environment, and its membrane is dominated by GDGTs. Here, we comprehensively analyzed the lipidome and transcriptome of S. acidocaldarius in the presence of terbinafine. Depletion of GDGTs and the accompanying accumulation of DGDs upon treatment with terbinafine were growth phase-dependent. Additionally, a major shift in the saturation of caldariellaquinones was observed, which resulted in the accumulation of unsaturated molecules. Transcriptomic data indicated that terbinafine has a multitude of effects, including significant differential expression of genes in the respiratory complex, motility, cell envelope, fatty acid metabolism, and GDGT cyclization. Combined, these findings suggest that the response of S. acidocaldarius to terbinafine inhibition involves respiratory stress and the differential expression of genes involved in isoprenoid biosynthesis and saturation.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Membrane Adaptations and Cellular Responses of Sulfolobus acidocaldarius to the Allylamine Terbinafine

Rao

Kok

Driessen

2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lipid extracts were analyzed by UHPLC-MS for the determination of the presence of various lipid species and their saturation state as described in detail elsewhere 61,62 . In brief, 5 µl of a lipid extract (10 mg/ml in methanol) was injected on a Waters Acquity CSH C18 column.…”

Section: Lipidomic Analysis Of the S Aureus Lipid Extractsmentioning

confidence: 99%

Lateral membrane organization as target of an antimicrobial peptidomimetic compound

Melcrová

Maity

Melcr

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Antimicrobial resistance is one of the leading concerns in medical care. Here we resolve the functional mechanism of the antimicrobial action of the cationic tripeptide AMC-109 by combining high speed-atomic force microscopy, molecular dynamics, fluorescence assays, and lipidomic analysis. We show that AMC-109 activity on the negatively charged plasma membrane of Staphylococcus aureus consists of two crucial steps. First, AMC-109 self-assembles into stable aggregates with specificity for negatively charged membranes. Second, by incorporation into the S. aureus membrane the lateral membrane organization is affected, dissolving membrane nanodomains. Domain dissolution affects membrane functions such as protein sorting and cell wall synthesis, and is suggested to cause a loss of resistance of methicillin-resistant S. aureus (MRSA) to methicillin. As the AMC-109 mode of action is similar to the activity of the disinfectant benzalkonium chloride (BAK), a broad applicability, but with low cytotoxicity to human cells, is expected.

show abstract

“…Voltage parameters were as follows: 3kV (spray), -75 V (capillary), -190 V (tube lens) and -46 V (Skimmer). Eluent A was MilliQ:MeCN (40:60) and Eluent B was MilliQ:MeCB:1-BuOH (0.5:10:90), both containing 5 mM ammonium formate [53]. A linear elution gradient was used: 55/45 eluent A/B for 2.5 minutes; 10/90 eluent A/B from 2.5 -24.5 mins; returning to 55/45 eluent A/B for 25-33 mins.…”

Section: Lipid Extraction and Analysesmentioning

confidence: 99%

Membrane Adaptations and Cellular Responses of Sulfolobus acidocaldarius to the allylamine terbinafine

Rao¹,

Driessen²

2022

Preprint

View full text Add to dashboard Cite

show abstract

A versatile method to separate complex lipid mixtures using 1-butanol as eluent in a reverse-phase UHPLC-ESI-MS system

Cited by 12 publications

References 71 publications

Membrane Adaptations and Cellular Responses of Sulfolobus acidocaldarius to the Allylamine Terbinafine

Membrane Adaptations and Cellular Responses of Sulfolobus acidocaldarius to the Allylamine Terbinafine

Lateral membrane organization as target of an antimicrobial peptidomimetic compound

Membrane Adaptations and Cellular Responses of Sulfolobus acidocaldarius to the allylamine terbinafine

Contact Info

Product

Resources

About