A Versatile Method to Prepare Protein Nanoclusters for Drug Delivery

Abstract

Nanocarriers based on natural biomaterials such as peptides and proteins have shown great advantages in the field of nanomedicine. However, the complicated preparation process and possible denaturation of proteins may limit their further applications. Herein, a novel method is developed to prepare protein nanocluster drug delivery system based on the self-aggregated property of proteins under the isoelectric point condition. The crosslinked protein nanoclusters, prepared by adding modified natural crosslinking agent polysaccharide, exhibit excellent stability and autofluorescent property in physiological conditions. Hemoglobin, a model protein, is chosen for preparation of drug-loaded nanoclusters. The as-prepared nanoclusters demonstrate a pH-responsive drug release behavior and can successfully deliver drugs into cancer cells. Moreover, this approach can be extended to various proteins, exemplifying the universal applicability of our new preparation method for protein-based nanoparticles.

In vitro antioxidant and antitumor study of zein/SHA nanoparticles loaded with resveratrol

“…Measured the release of resveratrol in vitro using the previously reported dialysis method. (Wang et al., 2018). Put 2 ml of RES loaded zein‐SHA NPs with a certain concentration into the dialysis bag.…”

Construction of a CRISPR‐Biolayer Interferometry Platform for Real‐Time, Sensitive, and Specific DNA Detection

“…Several strategies such as amino or carboxyl reaction, [33,34] click chemistry, [35] Schiff base, [36] and host-guest recognition are usually attempted for labelling protein. [37] In this study, after excluding methods requiring complicated defective expression or multiple-steps, we selected three facile approaches to modify the CRISPR protein with biotin: 1) reacting biotin-sulfo-NHS ester with amino groups of proteins; 2) binding carboxyl groups on dCas9 via EDC activation; and 3) using a site-specific modification or host-guest interaction.…”

Encapsulation of L‐dopa and catechol in bovine serum albumin nanocarrier using desolvation method and their in vitro release studies

“…Chromatogram of L-dopa (a) and catechol (b), after complete release of drug from BSA@drug NPs using methanol (1) and supernatant solution at the end of the nanoparticle preparation process (2)…”

“…[1] Proteins are regarded as biological polymers that originate from amino acids and among all the available materials, the versatile albumin is an ideal material to fabricate nanodrugs. [2] Albumin has been used as a drug deliverer due to several advantages. First, Human Serum Albumin (HSA) is recognized as an endogenous protein by the human body in addition to the facts that it is nontoxic and biodegradable, originally.…”