A versatile immobilization-free photoelectrochemical biosensor for ultrasensitive detection of cancer biomarker based on enzyme-free cascaded quadratic amplification strategy

Ge, Lei; Wang, Wenxiao; Hou, Ting; Li, Feng

doi:10.1016/j.bios.2015.09.041

Cited by 106 publications

(34 citation statements)

References 42 publications

(38 reference statements)

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“…The enzyme‐based biosensors have unique advantages, such as high specificity, good selectivity, reusability, low‐cost, facile preparation, and easy miniaturization. However, they suffer from some deficiencies, including instability, limited lifetime, irretrievable enzyme denaturation under the harsh experimental conditions, difficulties in storage, susceptibility to the sample matrix, and sensitivity to the temperature and pH variations (Ge et al, 2016; Y. Han et al, 2013; Othman et al, 2016; Rodríguez‐Delgado et al, 2015; Syshchyk et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Recent advances in computational methods for biosensor design

Khoshbin

Housaindokht

Izadyar

et al. 2020

Biotech & Bioengineering

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Biosensors are analytical tools with a great application in healthcare, food quality control, and environmental monitoring. They are of considerable interest to be designed by using cost‐effective and efficient approaches. Designing biosensors with improved functionality or application in new target detection has been converted to a fast‐growing field of biomedicine and biotechnology branches. Experimental efforts have led to valuable successes in the field of biosensor design; however, some deficiencies restrict their utilization for this purpose. Computational design of biosensors is introduced as a promising key to eliminate the gap. A set of reliable structure prediction of the biosensor segments, their stability, and accurate descriptors of molecular interactions are required to computationally design biosensors. In this review, we provide a comprehensive insight into the progress of computational methods to guide the design and development of biosensors, including molecular dynamics simulation, quantum mechanics calculations, molecular docking, virtual screening, and a combination of them as the hybrid methodologies. By relying on the recent advances in the computational methods, an opportunity emerged for them to be complementary or an alternative to the experimental methods in the field of biosensor design.

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Section: Introductionmentioning

confidence: 99%

Recent advances in computational methods for biosensor design

Khoshbin

Housaindokht

Izadyar

et al. 2020

Biotech & Bioengineering

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“…† In addition, this single-step detection strategy had a dramatically shorter incubation time in comparison with most of the previously reported amplification strategies. 26,27,35–37 …”

Section: Resultsmentioning

confidence: 99%

“… 25 For example, an aptamer transduction-responsive amplification strategy was developed for CEA detection based on catalytic hairpin assembly (CHA) and the hybridization chain reaction (HCR). 26 Similarly, a target binging-induced cascade amplification method was constructed for CEA detection using an Exo III-assisted DNA walker. 27 However, these methods suffer from long assay times and multi-step reactions.…”

Section: Introductionmentioning

confidence: 99%

Single-step and ultrasensitive detection of carcinoembryonic antigen based on an aptamer transduction-mediated exonuclease III-assisted dual-amplification strategy

Fan

et al. 2018

RSC Adv.

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“…Inorganic semiconductors such as titanium dioxide (TiO 2 ) (Tian et al, 2018), cadmium telluride (CdTe) (Lin et al, 2016; Hao et al, 2017b; Li et al, 2018a), cadmium sulfide (CdS) (Gong et al, 2015; Ge et al, 2016a; Lv et al, 2017), Molybdenum disulphide (MoS 2 ) (Zang et al, 2016; Wang et al, 2019), cadmium selenide (CdSe) (Matylitsky et al, 2009; Wang et al, 2014c), and zinc oxide (ZnO) (Han et al, 2017b) are used extensively in affinity based PEC biosensors (Hong et al, 2015; Lin et al, 2016; Hao et al, 2017b; Li et al, 2018a). Inorganic semiconductors offer a few advantages over their organic counterparts as discussed in the following section.…”

Section: Photoactive Species For Pec Biosensorsmentioning

confidence: 99%

Affinity-Based Detection of Biomolecules Using Photo-Electrochemical Readout

et al. 2019

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Detection and quantification of biologically-relevant analytes using handheld platforms are important for point-of-care diagnostics, real-time health monitoring, and treatment monitoring. Among the various signal transduction methods used in portable biosensors, photoelectrochemcial (PEC) readout has emerged as a promising approach due to its low limit-of-detection and high sensitivity. For this readout method to be applicable to analyzing native samples, performance requirements beyond sensitivity such as specificity, stability, and ease of operation are critical. These performance requirements are governed by the properties of the photoactive materials and signal transduction mechanisms that are used in PEC biosensing. In this review, we categorize PEC biosensors into five areas based on their signal transduction strategy: (a) introduction of photoactive species, (b) generation of electron/hole donors, (c) use of steric hinderance, (d) in situ induction of light, and (e) resonance energy transfer. We discuss the combination of strengths and weaknesses that these signal transduction systems and their material building blocks offer by reviewing the recent progress in this area. Developing the appropriate PEC biosensor starts with defining the application case followed by choosing the materials and signal transduction strategies that meet the application-based specifications.

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A versatile immobilization-free photoelectrochemical biosensor for ultrasensitive detection of cancer biomarker based on enzyme-free cascaded quadratic amplification strategy

Cited by 106 publications

References 42 publications

Recent advances in computational methods for biosensor design

Recent advances in computational methods for biosensor design

Single-step and ultrasensitive detection of carcinoembryonic antigen based on an aptamer transduction-mediated exonuclease III-assisted dual-amplification strategy

Affinity-Based Detection of Biomolecules Using Photo-Electrochemical Readout

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