A Versatile Gold Surface Approach for Fabrication and Interrogation of Glycoarrays

Abstract

Glycoarrays on gold: A designer gold surface incorporating a self-assembled monolayer with weak protein absorption properties has been optimised for rapid display and interrogation of both native and derivatised glycans in array formats. This rapid, facile approach has diverse applications in glycomics, through exploitation of fluorescence, SPR and MALDI-ToF MS detection methods

SPOT Synthesis of Peptide Arrays on Self‐Assembled Monolayers and their Evaluation as Enzyme Substrates

“…[2] SAMs present well-defined biocompatible surfaces that are easy to prepare and are amenable to detailed physicochemical analysis of molecular structure and binding interactions, in particular through MALDI-ToF MS [3,4,5] (matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry) and SPR [6,7,8] (surface plasmon resonance). So far, a broad range of chemical and biochemical reactions have been described [5,9] that are compatible with SAMs, but in all cases the number of consecutive reaction steps conducted on the surface has been small, [10,11] in particular in array format.…”

Synthesis of Benzaldehyde‐Functionalized Glycans: A Novel Approach Towards Glyco‐SAMs as a Tool for Surface Plasmon Resonance Studies

“…[52][53][54][55] In many cases SAMs are formed by the covalent binding of thiols to gold. [56][57][58][59] For example, Zhi et al reported a concept that could be applied to synthetic glycosides and glycans from natural sources. [57] However, the approach reported herein focused on well-defined synthetic saccharides.…”

“…[56][57][58][59] For example, Zhi et al reported a concept that could be applied to synthetic glycosides and glycans from natural sources. [57] However, the approach reported herein focused on well-defined synthetic saccharides. Recently, Penades and co-workers used long-chain alkenyl N-acetylglucosaminopyranosides and 11-mercapto-1-undecanol as a reference for the formation of SAMs.…”

Modulating patterned adhesion and repulsion of HEK 293 cells on microengineered parylene‐C/SiO2 substrates

“…1,2 It is also critical to applications such as tissue engineering, drug discovery, and the fabrication of biosensors. [3][4][5] An armamentarium of techniques is evolving that takes advantage of biological, chemical, and physical factors that influence cell adhesion.…”