A versatile family of degradable non-viral gene carriers based on hyperbranched poly(ester amine)s

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

“…We showed that an increasing number of carbon atoms in the aliphatic spacer of the diacrylates was able to increase DNA condensation ability, revealing that an increasing hydrophobicity of the pseudodendritic core plays an important role in DNA binding. This is consistent with previous work by Zhong et al, 7 who found that among their linear poly(ester amine)s investigated, the most hydrophobic ones showed the best DNA-binding ability. Furthermore, an increasing number of nitrogen per coupled oligoamine on the pseudodendritic surface resulted in stepwise rising B-potentials, which also led to enhanced DNA binding and condensation to nanoscaled polyplexes.…”

“…2,3 Thus, an excellent in vivo transfection activity at low toxicity should be the major considerations regarding the design of novel nonviral gene delivery devices. Recently, many degradable polymers have been generated, [4][5][6][7][8][9][10][11][12][13][14] aiming to reduce the toxicity profile while maintaining transfection efficiency levels comparable to nondegradable gene vectors like polyamidoamine dendrimers [15][16][17] or optimized polyethylenimines (PEIs). 18,19 For example, promising results have been obtained 6,9 by coupling low molecular weight oligoethylenimine 800 Da (OEI) with short diacrylate linkages, thus combining the beneficial low cytotoxic properties of OEI with the higher transfection efficiency of higher molecular weight PEIs.…”

Incorporation of 2,3‐Diaminopropionic Acid into Linear Cationic Amphipathic Peptides Produces pH‐Sensitive Vectors

“…the vector peptide LAH4 are uncharged at neutral pH but when the pH of the endosomal lumen drops, the side-chains become protonated. [3,4] Histidine rich peptides, as with other polyamines with high buffering capacities between pH 5.1 and pH 7.4, [5][6][7][8][9][10][11][12] are capable of acting as a proton sponge while the membrane disturbing behavior and nucleic acid binding ability of the peptide are also dramatically altered. [13,14] As a result of including the pH sensitive histidines, large numbers of membrane disturbing peptides are released from the nucleic acid-peptide complex during endosomal acidification enhancing the ability of the complex to escape the endosome and reach the cell cytosol and, eventually, nucleus.…”

Poly(ester amine) constructed from polyethylenimine and pluronic for gene delivery in vitro and in vivo

“…Such as Dr. Langer's group reported a large library of poly(ester amine)s (Lynn & Langer, 2000;Akinc et al, 2003;Anderson et al, 2004), which were prepared by the reaction of a primary amine or bis(secondary amine) with a diacrylate, and some of them were highly efficient in vitro and in vivo and still able to maintain degradability and low cytotoxicity. Zhong et al showed that hyperbranched poly(ester amine)s containing primary, secondary and tertiary amino groups were degradable and yet had a higher transfection efficiency with a lower cytotoxicity compared to PEI 25K in vitro (Zhong et al, 2005).…”