A versatile carrier for simply constructed wire knives and microsyringes

Scouten, Charles W.; Cegavske, Craig F.; Rozboril, Lloyd

doi:10.1016/0031-9384(81)90217-1

Cited by 20 publications

(5 citation statements)

References 19 publications

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“…Mechanical transection of the nigrostriatal tract results in the delayed degeneration of neurons in the substantia nigra (Hagg & Varon, 1993; Brecknell et al ., 1995). Adult male Wistar rats (PND42) were axotomized using a wire knife (Scouten et al ., 1981; David Kopf Instruments) lowered 8.0 mm ventral and 2.4 mm to the right and 3.8 mm caudal to bregma. The wire knife was then extruded 2 mm from the end of the blade carrier and raised 2.5 mm.…”

Section: Methodsmentioning

confidence: 99%

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NAIP protects the nigrostriatal dopamine pathway in an intrastriatal 6‐OHDA rat model of Parkinson's disease

Crocker

Wigle

Liston

et al. 2001

Eur J of Neuroscience

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Parkinson's disease (PD) is a progressive neurodegenerative disorder of the basal ganglia, associated with the inappropriate death of dopaminergic neurons of the substantia nigra pars compacta (SNc). Here, we show that adenovirally mediated expression of neuronal apoptosis inhibitor protein (NAIP) ameliorates the loss of nigrostriatal function following intrastriatal 6-OHDA administration by attenuating the death of dopamine neurons and dopaminergic fibres in the striatum. In addition, we also addressed the role of the cysteine protease caspase-3 activity in this adult 6-OHDA model, because a role for caspases has been implicated in the loss of dopamine neurons in PD, and because NAIP is also a reputed inhibitor of caspase-3. Although caspase-3-like proteolysis was induced in the SNc dopamine neurons of juvenile rats lesioned with 6-OHDA and in adult rats following axotomy of the medial forebrain bundle, caspase-3 is not induced in the dopamine neurons of adult 6-OHDA-lesioned animals. Taken together, these results suggest that therapeutic strategies based on NAIP may have potential value for the treatment of PD.

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Section: Methodsmentioning

confidence: 99%

“…Ad-NAIP-6-OHDA PND 42 ± ± ± ± ± ± ± ± ± d Ad-lacZ-6-OHDA PND 42 ± ± ± ± ± ± ± ± ± axotomized using a wire knife (Scouten et al, 1981; David Kopf Instruments) lowered 8.0 mm ventral and 2.4 mm to the right and 3.8 mm caudal to bregma. The wire knife was then extruded 2 mm from the end of the blade carrier and raised 2.5 mm.…”

Section: Adenovirusmentioning

confidence: 99%

NAIP protects the nigrostriatal dopamine pathway in an intrastriatal 6‐OHDA rat model of Parkinson's disease

Crocker

Wigle

Liston

et al. 2001

Eur J of Neuroscience

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“…The other approach involves the retrograde degeneration of dopaminergic neurons by axonal transection, where the axons of dopaminergic neurons are severed to induce their degeneration (Reis et al, 1978). As more than 90% of midbrain dopaminergic neurons send their axons to the ipsilateral striatum through the nigrostriatal pathway (Fass and Butcher, 1981), selective MFB axotomy using a retractable wire knife, as developed by Scouten and colleagues (Scouten et al, 1981), leads to the degeneration of a significant number of dopaminergic neurons in the substantia nigra pars compacta within the midbrain (Sugama et al, 2003).…”

Section: Mfb Axotomy Modelmentioning

confidence: 99%

Medial Forebrain Bundle Axotomy Induced Dopaminergic Neuronal Degeneration is Accompanied with Microglial Activation

Kim,

Song,

Choy

2023

IJAR

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Background: We aimed to investigate the role of microglia in the degeneration of dopaminergic neurons by using the medial forebrain bundle (MFB) axotomy model, one of the animal models of Parkinson’s disease. Methods: We performed immunohistochemical staining, immunofluorescence staining, and Western blot assays on rats sacrificed 7, 14, and 28 days after the axotomy of their MFB to study tissue immune responses. Animal sacrifice was performed 30 minutes prior to injection of hydroethidine into the abdominal cavity to investigate the presence of oxidative stress in the brain tissue and the characteristics of cells that produce reactive oxygen species (ROS). Result: Using OX42 and OX6 antibodies, excessive activation of microglia occurred in the surgical site of the substantia nigra on 7 days after the procedure. Activated microglia were still present and clustered even in the 14-day and 28-day groups, although their reactivity had slightly decreased compared to the 7-day group. These cells showed strong immunopositive reactions to ED1, indicating that they were actively engaged in phagocytic activity. Many activated microglia showing positive immunofluorescence staining for OX6 also expressed ethidium fluorescence, indicating that these cells were actively generating and secreting ROS.

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“…An incision was made in the scalp and a small flap of skull (2.5 mm in diameter) removed to expose the superior sagittal sinus. The sinus was retracted and a Scouten w'ire microknife [33] lowered to a point aimed midw'ay between the SCN and the PVN (5.5-5.6 mm ventral to the sinus). The blade was then extended and rotated 360° in each direction.…”

Section: Surgerymentioning

confidence: 99%

Prolactin Secretion in Female Siberian Hamsters following Hypothalamic Deafferentation Role of Photoperiod and Dopamine

Badura¹,

Goldman²

1994

Neuroendocrinology

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The role of the hypothalamic paraventricular nucleus (PVN) region in the control of seasonal prolactin (PRL) responses was investigated in female Siberian hamsters via disruption of PVN afferent connections from the region of the suprachiasmatic nucleus (SCN). Adult female hamsters received sham surgery or horizontal knife cuts placed ventral to the PVN so as to sever dorsally projecting fibers from the SCN and were either immediately transferred to a short-day photoperiod [10 h light: 14 h dark (10L: 14D); experiment (exp.) 1A] or returned to the long-day photoperiod (16L:8D; exp. 1B and 2). Serial blood samples were analyzed for determination of circulating PRL and follicle-stimulating hormone (FSH; exp. 1 A) levels at various time points after surgery. In exp. 1 A, sham-operated animals showed a steady decline in circulating levels of both PRL and FSH during exposure to 10L. Animals with knife cuts that passed through the extent of the SCN were prevented from showing declines in PRL and FSH during exposure to 10L. Animals with knife cuts located ventral to or through the PVN but dorsal to the SCN maintained high FSH levels during short-day exposure but showed a decline in PRL. Similarly, animals with knife cuts in exp. IB and 2 showed a decline in circulating PRL despite continued exposure to a stimulatory photoperiod. In exp. 2, the efficacy of a dopaminergic agonist (CB154) and an antagonist (pimozide) in altering circulating PRL under the 16L photoperiod was evaluated. CB154 induced declines in PRL in knife-cut but not sham-operated animals, whereas administration of pimozide elevated circulating PRL in both groups. These results suggest that disruption of afferent input to the PVN region may result in reductions in circulating PRL independently of the photoperiodic regulation of this hormone, and via mechanisms other than increased dopaminergic tone, i.e., possibly through disruption of a hypothalamic PRL-stimulating system.

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A versatile carrier for simply constructed wire knives and microsyringes

Cited by 20 publications

References 19 publications

NAIP protects the nigrostriatal dopamine pathway in an intrastriatal 6‐OHDA rat model of Parkinson's disease

NAIP protects the nigrostriatal dopamine pathway in an intrastriatal 6‐OHDA rat model of Parkinson's disease

Medial Forebrain Bundle Axotomy Induced Dopaminergic Neuronal Degeneration is Accompanied with Microglial Activation

Prolactin Secretion in Female Siberian Hamsters following Hypothalamic Deafferentation Role of Photoperiod and Dopamine

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