A vehicle for photodynamic therapy of skin cancer: influence of dimethylsulphoxide on 5-aminolevulinic acid in vitro cutaneous permeation and in vivo protoporphyrin IX accumulation determined by confocal microscopy

Rosa, Fernanda Scarmato De; Marchetti, Juliana Maldonado; Thomazini, José Antônio; Tedesco, Antônio Cláudio; Bentley, Maria Vitória Lopes Badra

doi:10.1016/s0168-3659(99)00213-8

Cited by 121 publications

(77 citation statements)

References 28 publications

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“…10,11 5-aminolevulinic acid (ALA) is a precursor of PpIX, which is well known as a strong photosensitizer, and administered ALA is interconverted to PpIX via the heme biosynthetic pathway. [12][13][14][15] PpIX accumulation in tumor cells is generally higher than that in normal cells, as the activity of key enzymes in tumor cells is significantly changed.…”

Section: Introductionmentioning

confidence: 99%

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Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Kim

Chung²,

Choi³

et al. 2011

IJN

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Cancer cells have been reported to exhibit an enhanced capacity for protoporphyrin IX (PpIX) synthesis facilitated by the administration of 5-aminolevulinic acid (ALA). We investigated the effect of ALA-based photodynamic therapy (PDT) on human cholangiocarcinoma cells (HuCC-T1). Since protoporphyrin IX (PpIX), a metabolite of ALA, can produce reactive oxygen species (ROS) under irradiation and then induce phototoxicity, ALA-based PDT is a promising candidate for the treatment of cholangiocarcinoma. When various concentrations of ALA (0.05–2 mM) were used to treat HuCC-T1 cells for 6 or 24 hours, the intracellular PpIX level increased according to the ALA concentration and treatment time. Furthermore, an increased amount of PpIX in HuCC-T1 cells induced increased production of ROS by irradiation, resulting in increased phototoxicity.

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Section: Introductionmentioning

confidence: 99%

“…This fact reduces or avoids the risk of prolonged phototoxicity to the human body. [14][15][16][17] Recently, researchers reported the effects of ALA-based PDT in various tumor cells such as oral squamous cell carcinoma, melanoma, glioma, lymphoma, esophageal carcinoma, and basal cell carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Kim

Chung²,

Choi³

et al. 2011

IJN

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“…The physicochemical characteristics of the PpIX allows the development of an analytical method for its quantification based on fluorescence spectroscopy, since this PS is able to emit fluorescence after excitation by a beam of light with an adequate wavelength (Castano, Deminova, Hamblin, 2004;De Rosa et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Fluorometric quantification of protoporphyrin IX in biological skin samples from in vitro penetration/permeation studies

Rossetti

Depieri

Tedesco

et al. 2010

Braz. J. Pharm. Sci.

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A fluorometric analytical method was developed for quantification of protoporphyrin IX (PpIX) in skin samples and receptor phase solution after in vitro cutaneous penetration/permeation studies. Analytical conditions used were: excitation and emission wavelengths: 400 nm and 632 nm; bandwidth: 0.5 nm; excitation and emission slits: 10/10. PpIX was recovered from two different layers of skin, the stratum corneum (SC) and the epidermis plus dermis ([E+D]), by vortex homogenization, probe and bath sonication, using DMSO as an extraction solvent. The detection and quantification limits were 0.002 and 0.005 µg/mL, respectively. The assay was linear from 0.005 -0.5 µg/mL. The within-day and betweenday assay precision and accuracy in DMSO and receptor phase solution were each studied at the two concentration levels 0.04 and 0.2 µg/mL, and 0.01 and 0.08 µg/mL, respectively. The coefficients of variation and deviation from the theoretical values were lower than 5%. The skin recovery of PpIX from SC and [E+D] layers using two different concentrations (0.5 and 1.0 µg/mL) were all above 90.0%. The method described has potential application to in vitro penetration/permeation studies of PpIX using porcine skin as a biological membrane model. Uniterms: Fluorometric quantification. Protoporphyrin IX/determination. Photodynamic therapy. Porcine skin. Cutaneous penetration/in vitro study. Cutaneous permeation/in vitro study.Um método analítico por espectrofluorimetria foi desenvolvido para quantificar a protoporfirina IX (Pp IX) em amostras de pele e fase receptora após a realização de testes in vitro de penetração/permeação cutâneas. As condições analíticas utilizadas foram: comprimentos de onda de excitação e emissão: 400 nm e 632 nm; largura de banda: 0,5 nm; fendas de excitação e emissão: 10/10. A PpIX foi extraída de amostras de estrato córneo (EC) e da epiderme sem estrato córneo + derme ([E+D]) através da agitação em vórtex e sonicação por haste e banho, utilizando-se o DMSO como solvente extrator. O limite de detecção e quantificação foram, respectivamente, de 0,002 e 0,005 µg/mL. O método mostrou-se linear da faixa de 0,005 -0,5 µg/mL. A precisão e exatidão intra e inter-ensaio em DMSO e na fase receptora foram validadas utilizando-se duas concentrações distintas, respectivamente, de 0,004 e 0,2 µg/mL, e 0,01 e 0,08 µg/mL. Os valores de coeficiente de variação e o desvio do valor teórico foram inferiores a 5%. A recuperação da PpIX das camadas da pele (EC e [E+D]) utilizando-se duas concentrações distintas (0,5 e 1,0 µg/mL) foram todas acima de 90,0%. O método descrito pode ser utilizado para determinação da PpIX após estudos de penetração/permeação cutânea in vitro utilizando pele de porco como modelo de membrana.Unitermos: Espectrofluorimetria/análise quantitativa. Quantificação fluorimétrica. Protoporfirina IX/ determinação. Terapia fotodinâmica. Pele porcina. Penetração cutânea/estudo in vitro. Permeação cutânea/estudo in vitro.

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“…DMSO is an enhancer of ALA penetration in tissue, and EDTA inhibits the enzyme ferrochelatase, resulting in a higher concentration of PpIX in cells. The PpIX production occurs inside the mitochondria, and it is induced by the ALA present in the biosynthetic heme pathway [18][19][20].…”

Section: Photosensitizationmentioning

confidence: 99%

Identification of skin lesions through aminolaevulinic acid-mediated photodynamic detection

Andrade

Vollet‐Filho

Sálvio

et al. 2014

Photodiagnosis and Photodynamic Therapy

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A vehicle for photodynamic therapy of skin cancer: influence of dimethylsulphoxide on 5-aminolevulinic acid in vitro cutaneous permeation and in vivo protoporphyrin IX accumulation determined by confocal microscopy

Cited by 121 publications

References 28 publications

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells

Fluorometric quantification of protoporphyrin IX in biological skin samples from in vitro penetration/permeation studies

Identification of skin lesions through aminolaevulinic acid-mediated photodynamic detection

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