2013
DOI: 10.1073/pnas.1218705110
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A vector-free microfluidic platform for intracellular delivery

Abstract: Intracellular delivery of macromolecules is a challenge in research and therapeutic applications. Existing vector-based and physical methods have limitations, including their reliance on exogenous materials or electrical fields, which can lead to toxicity or off-target effects. We describe a microfluidic approach to delivery in which cells are mechanically deformed as they pass through a constriction 30-80% smaller than the cell diameter. The resulting controlled application of compression and shear forces res… Show more

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Cited by 396 publications
(505 citation statements)
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“…1, we aim to address how a nanosize MS channel in a model bilayer can be activated by flow-generated stresses by focusing on two typical physiological flows involving vesicles: a planar shear flow and flow through a narrowing constriction. For example, recently a microfluidic platform has been reported for intracellular delivery of macromolecules into a highly deformed cell as it passes through a narrow constriction (26). Here we illustrate that a similar microfluidic constriction flow can gate a MS channel open for intracellular delivery.…”
mentioning
confidence: 60%
“…1, we aim to address how a nanosize MS channel in a model bilayer can be activated by flow-generated stresses by focusing on two typical physiological flows involving vesicles: a planar shear flow and flow through a narrowing constriction. For example, recently a microfluidic platform has been reported for intracellular delivery of macromolecules into a highly deformed cell as it passes through a narrow constriction (26). Here we illustrate that a similar microfluidic constriction flow can gate a MS channel open for intracellular delivery.…”
mentioning
confidence: 60%
“…Anderson and Knothe Tate [44] report that such a stress state will result in stem cells differentiation towards an endothelial lineage. The characterisation of nucleus behaviour presented in the current study may aid the development and calibration of microfluidic devices for cell sorting [45] and direct cytosolic delivery of transcription factors [46].…”
Section: Discussionmentioning
confidence: 96%
“…34 Unlike these previous approaches, the plasmids in our study were not packaged or pre-adsorbed on a material surface before cell seeding. Instead, the cells and plasmids were plated at the same time ('concurrent' transfection) on the nanosheets to improve the transfection efficiency.…”
Section: Resultsmentioning
confidence: 99%