A variety of wheat tolerant to deep‐seeding conditions: elongation of the first internode depends on the response to gibberellin and potassium

Chen, L.; Nishizawa, T.; Higashitani, Akihiro; Suge, Hiroshi; Wakui, Yuta; Takeda, Kazuyoshi; Takahashi, Hideyuki

doi:10.1046/j.1365-3040.2001.00688.x

Cited by 28 publications

(45 citation statements)

References 36 publications

(53 reference statements)

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“…Orthologous proteins have since been identified in other grasses, such as rice, wheat, and Lolium temulentum (Gubler et al, 1997;Gocal et al, 1999;Chen et al, 2001). The GAMYB protein induces the expression of ␣ -amylase and some other GA-inducible genes by interacting directly with the GA-responsive cisacting elements of these genes in aleurone tissue .…”

Section: Introductionmentioning

confidence: 99%

Loss-of-Function Mutations of the Rice GAMYB Gene Impair α-Amylase Expression in Aleurone and Flower Development

et al. 2003

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GAMYB was first isolated as a positive transcriptional regulator of gibberellin (GA)-dependent ␣ -amylase expression in barley aleurone cells, and its molecular and biochemical properties have been well characterized. However, the role of GAMYB elsewhere in the plant is not well understood. To investigate the molecular function of GAMYB outside of the aleurone cells, we isolated loss-of-function mutants from a panel of rice mutants produced by the insertion of a retrotransposon, Tos17 . Through PCR screening using primers for rice GAMYB ( OsGAMYB ) and Tos17 , we isolated three independent mutant alleles that contained Tos17 inserted in the exon region. No ␣ -amylase expression in the endosperm was induced in these mutants in response to GA treatment, indicating that the Tos17 insertion had knocked out OsGAMYB function. We found no significant defects in the growth and development of the mutants at the vegetative stage. After the phase transition to the reproductive stage, however, shortened internodes and defects in floral organ development, especially a defect in pollen development, were observed. On the other hand, no difference was detected in flowering time. High-level OsGAMYB expression was detected in the aleurone cells, inflorescence shoot apical region, stamen primordia, and tapetum cells of the anther, but only low-level expression occurred in organs at the vegetative stage or in the elongating stem. These results demonstrate that, in addition to its role in the induction of ␣ -amylase in aleurone, OsGAMYB also is important for floral organ development and essential for pollen development.

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Section: Introductionmentioning

confidence: 99%

Loss-of-Function Mutations of the Rice GAMYB Gene Impair α-Amylase Expression in Aleurone and Flower Development

et al. 2003

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“…Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.104.027920. (Diaz et al, 2002), elongation of the first internode in wheat (Triticum aestivum) (Chen et al, 2001), and flowering in Lolium temulentum (Gocal et al, 1999).In Arabidopsis, there is a small family of GAMYB-like genes (Gocal et al, 2001). Construction of a phylogenetic tree with the entire MYB gene family of Arabidopsis groups five genes (MYB33, MYB65, MYB97, MYB101, and MYB120) with HvGAMYB (Stracke et al, 2001).…”

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confidence: 99%

The Arabidopsis GAMYB-Like Genes, MYB33 and MYB65, Are MicroRNA-Regulated Genes That Redundantly Facilitate Anther Development

2005

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The functions of the vast majority of genes encoding R2R3 MYB domain proteins remain unknown. The closely related MYB33 and MYB65 genes of Arabidopsis thaliana have high sequence similarity to the barley (Hordeum vulgare) GAMYB gene. T-DNA insertional mutants were isolated for both genes, and a myb33 myb65 double mutant was defective in anther development. In myb33 myb65 anthers, the tapetum undergoes hypertrophy at the pollen mother cell stage, resulting in premeiotic abortion of pollen development. However, myb33 myb65 sterility was conditional, where fertility increased both under higher light or lower temperature conditions. Thus, MYB33/MYB65 facilitate, but are not essential for, anther development. Neither single mutant displayed a phenotype, implying that MYB33 and MYB65 are functionally redundant. Consistent with functional redundancy, promoter-b-glucuronidase (GUS) fusions of MYB33 and MYB65 gave identical expression patterns in flowers (sepals, style, receptacle, anther filaments, and connective but not in anthers themselves), shoot apices, and root tips. By contrast, expression of a MYB33:GUS translational fusion in flowers was solely in young anthers (consistent with the male sterile phenotype), and no staining was seen in shoot meristems or root tips. A microRNA target sequence is present in the MYB genes, and mutating this sequence in the MYB33:GUS fusion results in an expanded expression pattern, in tissues similar to that observed in the promoter-GUS lines, implying that the microRNA target sequence is restricting MYB33 expression. Arabidopsis transformed with MYB33 containing the mutated microRNA target had dramatic pleiotrophic developmental defects, suggesting that restricting MYB33 expression, especially in the shoot apices, is essential for proper plant development. INTRODUCTIONWith >120 genes in the Arabidopsis thaliana genome, the R2R3-MYB gene family has been identified as one of the most abundant classes of transcription factors in plants (Stracke et al., 2001). They are involved in a diverse range of processes, including controlling cell shape, disease resistance, regulating secondary metabolism, and hormone signal transduction (Jin and Martin, 1999). One MYB gene from barley (Hordeum vulgare), HvGAMYB, is involved in gibberellin (GA) signaling in the aleurone (Gubler et al., 1995). Here, expression of HvGAMYB is upregulated by GA, where it then binds to the TAACAAA motif of a barley high-pI a-amylase promoter, a motif that plays an important role in the GA-regulated expression of the a-amylase gene (Skriver et al., 1991;Gubler and Jacobsen, 1992;Lanahan et al., 1992). In transient assays, constitutive expression of HvGAMYB is sufficient to activate the a-amylase promoter (Gubler et al., 1995) and the promoters of other aleurone GAregulated genes that are required for the mobilization of endosperm reserves (Cercó s et al., 1999;Gubler et al., 1999). Thus, in regards to expression of these genes in aleurone layers, transient expression of HvGAMYB has the same effect as GA application. Fr...

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“…Tall-type cultivars of wheat responded to the applied GA 3 by slightly elongating the first internode, but the stimulation by GA 3 was only 19. 2% of that in Hong Mang Mai (Chen et al, 2001). We measured the levels of endogenous GAs, but there was no significant difference in GA content among the cultivars tested, including Hong Mang Mai (Chen et al,200 I) .…”

Section: Discussionmentioning

confidence: 94%

“…The elongation of the first internode in Hong Mang Mai is also enhanced by the application of GA and potassium (Chen et al, 2001). In the present study, we further studied this phenomenon from a more agronomic point of view.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Responses of the First Internodes of Hong Mang Mai Wheat to Ethylene, Gibberellins and Potassium

Nishizawa¹,

Chen²,

Higashitani³

et al. 2002

Plant Production Science

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A variety of wheat tolerant to deep‐seeding conditions: elongation of the first internode depends on the response to gibberellin and potassium

Cited by 28 publications

References 36 publications

Loss-of-Function Mutations of the Rice GAMYB Gene Impair α-Amylase Expression in Aleurone and Flower Development

Loss-of-Function Mutations of the Rice GAMYB Gene Impair α-Amylase Expression in Aleurone and Flower Development

The Arabidopsis GAMYB-Like Genes, MYB33 and MYB65, Are MicroRNA-Regulated Genes That Redundantly Facilitate Anther Development

Responses of the First Internodes of Hong Mang Mai Wheat to Ethylene, Gibberellins and Potassium

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