A VAMP-associated protein, PVA31 is involved in leaf senescence in<i>Arabidopsis</i>

Ichikawa, Mie; Nakai, Yusuke; Arima, Keita; Nishiyama, Sayo; Hirano, Toshio; Sato, Masa H.

doi:10.4161/15592324.2014.990847

Cited by 16 publications

(9 citation statements)

References 41 publications

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“…SNAREs are small proteins often inserted into membranes that can function on either target membranes (t-SNAREs) or transport vesicles (v-SNAREs) [ 14 ]. Vesicle-associated membrane proteins or VAMPs (also referred to as synaptobrevins), are the transport vesicle residing SNARE proteins involved in the membrane fusion processes between transport vesicles and target membranes [ 15 ]. Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 (syntaxin), SNAP33 and endomembrane-resident VAMP721/722 [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

A genome-wide identification and comparative analysis of the lentil MLO genes

et al. 2018

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Powdery mildew is a widespread fungal plant disease that can cause significant losses in many crops. Some MLO genes (Mildew resistance locus O) have proved to confer a durable resistance to powdery mildew in several species. Resistance granted by the MLO gene family members has prompted an increasing interest in characterizing these genes and implementing their use in plant breeding. Lentil (Lens culinaris Medik.) is a widely grown food legume almost exclusively consumed as dry seed with an average world production of 4.5 million tons. Powdery mildew causes severe losses on certain lentil cultivars under particular environmental conditions. Data mining of the lentil CDC Redberry draft genome allowed to identify up to 15 gene sequences with homology to known MLO genes, designated as LcMLOs. Further characterization of these gene sequences and their deduced protein sequences demonstrated conformity with key MLO protein characteristics such as the presence of transmembrane and calmodulin binding domains, as well as that of other conserved motifs. Phylogenetic and other comparative analyses revealed that LcMLO1 and LcMLO3 are the most likely gene orthologs related to powdery mildew response in other species, sharing a high similarity with other known resistance genes of dicot species, such as pea PsMLO1 and Medicago truncatula MtMLO1 and MtMLO3. Sets of primers were designed as tools to PCR amplify the genomic sequences of LcMLO1 and LcMLO3, also to screen lentil germplasm in search of resistance mutants. Primers were used to obtain the complete sequences of these two genes in all of the six wild lentil relatives. Respective to each gene, all Lens sequences shared a high similarity. Likewise, we used these primers to screen a working collection of 58 cultivated and 23 wild lentil accessions in search of length polymorphisms present in these two genes. All these data widen the insights on this gene family and can be useful for breeding programs in lentil and close related species.

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Section: Introductionmentioning

confidence: 99%

A genome-wide identification and comparative analysis of the lentil MLO genes

et al. 2018

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“…PEN1 in plants forms the SNARE complex with VAMP721 and VAMP722 during defense against powdery mildew fungi, and it also forms SNARE complexes in vitro with VAMP724 and VAMP727, which are not related to plant immunity ( Kwon et al, 2008 ). PVA31 participates in SA-associated apoptosis by interacting with VAMP721/722/724 but not VAMP711/727 to combat pathogen infection ( Ichikawa et al, 2015 ). In wheat, silencing of TaNPSN11/13 reduced the resistance to CYR23 , whereas silencing of TaNPSN12 did not have the same effect ( Wang et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, evolution, and expression of the SNARE gene family in wheat resistance to powdery mildew

Wang

Long

et al. 2021

PeerJ

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SNARE proteins mediate eukaryotic cell membrane/transport vesicle fusion and act in plant resistance to fungi. Herein, 173 SNARE proteins were identified in wheat and divided into 5 subfamilies and 21 classes. The number of the SYP1 class type was largest in TaSNAREs. Phylogenetic tree analysis revealed that most of the SNAREs were distributed in 21 classes. Analysis of the genetic structure revealed large differences among the 21 classes, and the structures in the same group were similar, except across individual genes. Excluding the first homoeologous group, the number in the other homoeologous groups was similar. The 2,000 bp promoter region of the TaSNARE genes were analyzed, and many W-box, MYB and disease-related cis-acting elements were identified. The qRT-PCR-based analysis of the SNARE genes revealed similar expression patterns of the same subfamily in one wheat variety. The expression patterns of the same gene in resistant/sensitive varieties largely differed at 6 h after infection, suggesting that SNARE proteins play an important role in early pathogen infection. Here, the identification and expression analysis of SNARE proteins provide a theoretical basis for studies of SNARE protein function and wheat resistance to powdery mildew.

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“…Manuscript to be reviewed participates in SA-associated apoptosis by interacting with VAMP721/722/724 but not VAMP711/727 to combat pathogen infection (Ichikawa et al 2015). In wheat, silencing of TaNPSN11/13 reduced the resistance to CYR23, whereas silencing of TaNPSN12 did not have the same effect (Wang et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Genome-wide identification, evolution, and expression of the SNARE gene family in wheat resistance to powdery mildew (v0.1)"

Mukhopadhyay¹

2021

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SNARE proteins mediate eukaryotic cell membrane/transport vesicle fusion and act in plant resistance to fungi. Herein, 173 SNARE proteins were identified in wheat and divided into 5 subfamilies and 21 classes. The number of the SYP1 class type was largest in TaSNAREs. Phylogenetic tree analysis revealed that most of the SNAREs were distributed in 21 classes. Analysis of the genetic structure revealed large differences among the 21 classes, and the structures in the same group were similar, except across individual genes. Excluding the first homoeologous group , the number in the other homoeologous groups was similar. The 2000 bp promoter region of the TaSNARE genes were analyzed, and many W-box, MYB and disease-related cis-acting elements were identified. The qRT-PCR-based analysis of the SNARE genes revealed similar expression patterns of the same subfamily in one wheat variety. The expression patterns of the same gene in resistant/sensitive varieties largely differed at 6h after infection, suggesting that SNARE proteins play an important role in early pathogen infection. Here, the identification and expression analysis of SNARE proteins provide a theoretical basis for studies of SNARE protein function and wheat resistance to powdery mildew.

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A VAMP-associated protein, PVA31 is involved in leaf senescence inArabidopsis

Cited by 16 publications

References 41 publications

A genome-wide identification and comparative analysis of the lentil MLO genes

A genome-wide identification and comparative analysis of the lentil MLO genes

Genome-wide identification, evolution, and expression of the SNARE gene family in wheat resistance to powdery mildew

Peer Review #1 of "Genome-wide identification, evolution, and expression of the SNARE gene family in wheat resistance to powdery mildew (v0.1)"

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