A validated UHPLC-MS/MS method for pharmacokinetic and brain distribution studies of twenty constituents in rat after oral administration of Jia-Wei-Qi-Fu-Yin

An, Hai-Ming; Li, Meng-Ning; Yang, Hua; Pang, Haijun; Qu, Cheng; Xu, Yangsheng; Liu, Run‐Zhou; Peng, Chao; Li, Ping; Gao, Wen

doi:10.1016/j.jpba.2021.114140

Cited by 16 publications

(8 citation statements)

References 23 publications

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“…Studies have shown that glycyrrhetinic acid [ 57 ], 3,6′-disinapoyl sucrose [ 63 ], tenuifolin [ 64 ], and senkyunolide I and H [ 65 ] can be absorbed into cerebrospinal fluid. Some components have been determined in the brain tissue homogenate [ 66 – 68 ], but whether these components can penetrate the BBB is unknown, and they may only exist in the astrocytes and/or vascular endothelial cells constituting the BBB. In this study, 3,6′-disinapoyl sucrose, ginsenoside Rh 1 , butylphthalide, glycyrrhetinic acid, tenuifolin, and senkyunolide I and H were detected in cerebrospinal fluid.…”

Section: Discussionmentioning

confidence: 99%

Identification of Chemical Components of Qi-Fu-Yin and Its Prototype Components and Metabolites in Rat Plasma and Cerebrospinal Fluid via UPLC-Q-TOF-MS

Zhao

Yang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Qi-Fu-Yin, a traditional Chinese medicine formula, has been used to treat Alzheimer’s disease (AD, a neurodegenerative disorder) in clinical setting. In this study, the chemical components of Qi-Fu-Yin and its prototype components and metabolites in rat plasma and cerebrospinal fluid, after oral administration, were preliminarily characterized via ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS). A total of 180 compounds, including saponins, flavonoids, organic acids, sucrose esters, oligosaccharide esters, phthalides, phenylethanoid glycosides, alkaloids, xanthones, terpene lactones, ionones, and iridoid glycoside, were tentatively characterized. For the first time, 51 prototypical components and 26 metabolites, including saponins, phthalides, flavonoids, sucrose esters, organic acids, alkaloids, ionones, terpene lactones, iridoid glycoside, and their derivatives, have been tentatively identified in the plasma. Furthermore, 10 prototypical components (including butylidenephthalide, butylphthalide, 20(S)-ginsenoside Rh1, 20(R)-ginsenoside Rh1, and zingibroside R1) and 6 metabolites were preliminarily characterized in cerebrospinal fluid. These results were beneficial to the discovery of the active components of Qi-Fu-Yin anti-AD.

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Section: Discussionmentioning

confidence: 99%

Identification of Chemical Components of Qi-Fu-Yin and Its Prototype Components and Metabolites in Rat Plasma and Cerebrospinal Fluid via UPLC-Q-TOF-MS

Zhao

Yang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…In addition, ginsenosides Rb1, Rg1, Ro, and Re can be detected in rat brain tissue after oral administration of Jia-Wei-Qi-Fu-Yin, a TCM decoction with ginseng as the main gradient. These data indicate that ginsenosides can penetrate through the blood–brain barrier (BBB) [ 36 ]. However, another study found that the average brain concentration of ginsenosides Rb1, Rg1, and Re was eight to 15 times lower than the corresponding content in plasma after the oral administration of ginseng extract in rats, which indicated that they have poor permeability to the BBB [ 37 ].…”

Section: Classification Chemical Structure and Pharmacokinetics Of Gi...mentioning

confidence: 99%

Ginsenoside and Its Therapeutic Potential for Cognitive Impairment

et al. 2022

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Cognitive impairment (CI) is one of the major clinical features of many neurodegenerative diseases. It can be aging-related or even appear in non-central nerve system (CNS) diseases. CI has a wide spectrum that ranges from the cognitive complaint with normal screening tests to mild CI and, at its end, dementia. Ginsenosides, agents extracted from a key Chinese herbal medicine (ginseng), show great promise as a new therapeutic option for treating CI. This review covered both clinical trials and preclinical studies to summarize the possible mechanisms of how ginsenosides affect CI in different diseases. It shows that ginsenosides can modulate signaling pathways associated with oxidative stress, apoptosis, inflammation, synaptic plasticity, and neurogenesis. The involved signaling pathways mainly include the PI3K/Akt, CREB/BDNF, Keap1/Nrf2 signaling, and NF-κB/NLRP3 inflammasome pathways. We hope to provide a theoretical basis for the treatment of CI for related diseases by ginsenosides.

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“…In addition, the liver first-pass effect of alisol A was significant (HD = 9.55%). Alisol A mainly produced hydroxylation (alisol+O) and dehydrogenation products (alisol-2H) through in vivo metabolism [5] and had high exposure in the liver (Figures S9-S11). Alisol A undergoes phase I metabolism in liver microsomes, and CYP450 plays a vital role in the metabolism of alisol A [31].…”

Section: Analysis Of Pharmacokinetic Hd and Heart Tissue Distributionmentioning

confidence: 99%

“…Although previous studies have revealed that QLQX exhibits a major improvement on heart function [3,4], the effective substances remain unclear. Pharmacokinetic and tissue distribution studies of TCMP are highly beneficial in forecasting effective substances and explaining their effectiveness mechanisms [5]. Furthermore, with significant in vivo exposure and strong pharmacodynamic activity, these components of TCMP have a good chance of being effective substances [6].…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics, hepatic disposition, and heart tissue distribution of 14 compounds in rat after oral administration of Qi‐Li‐Qiang‐Xin capsule via ultra‐high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry

Tang

Dai

Zeng

et al. 2022

J of Separation Science

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In the present study, a specific and sensitive approach using ultra-highperformance liquid chromatography coupled with triple quadrupole tandem mass spectrometry was developed and validated for the quantitative analysis of 14 constituents in rat plasma, liver, and heart. The method was fully validated and successfully applied to pharmacokinetic, hepatic disposition, and heart tissue distribution studies of 14 compounds after the oral administration of Qi-Li-Qiang-Xin capsule. Ginsenoside Rb1, alisol A, astragaloside IV, and periplocymarin were found to be highly exposed in rat plasma, while toxic components such as hypaconitine, mesaconitine, and periplocin had low circulation levels in vivo. Moreover, sinapine thiocyanate, neoline, formononetin, calycosin, and alisol A exhibited significant liver first-pass effects. Notably, high levels of alisol A, periplocymarin, benzoylmesaconine, and benzoylhypaconine were observed in the heart. Based on high exposure and appropriate pharmacokinetic features in the systemic plasma and heart, astragaloside IV, ginsenoside Rb1, periplocymarin, benzoylmesaconine, benzoylhypaconine, and alisol A can be considered as the main potentially effective components. Ultimately, the results provide relevant information for discovery of effective substances, as well as further anti-heart failure action mechanism investigations of Qi-Li-Qiang-Xin capsule.

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A validated UHPLC-MS/MS method for pharmacokinetic and brain distribution studies of twenty constituents in rat after oral administration of Jia-Wei-Qi-Fu-Yin

Cited by 16 publications

References 23 publications

Identification of Chemical Components of Qi-Fu-Yin and Its Prototype Components and Metabolites in Rat Plasma and Cerebrospinal Fluid via UPLC-Q-TOF-MS

Identification of Chemical Components of Qi-Fu-Yin and Its Prototype Components and Metabolites in Rat Plasma and Cerebrospinal Fluid via UPLC-Q-TOF-MS

Ginsenoside and Its Therapeutic Potential for Cognitive Impairment

Pharmacokinetics, hepatic disposition, and heart tissue distribution of 14 compounds in rat after oral administration of Qi‐Li‐Qiang‐Xin capsule via ultra‐high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry

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