A validated reverse‐phase HPLC analytical method for the quantification of phenolic compounds in Baccharis dracunculifolia

Abstract

The method presented good results for the following parameters: selectivity, linearity, accuracy, precision, robustness, as well as limit of detection and limit of quantitation. Therefore, this method could be considered as an analytical tool for the quality control of B. dracunculifolia raw material and its products in both cosmetic and pharmaceutical companies.

Ultrasound-assisted solid liquid extraction (USLE) of olive fruit (Olea europaea) phenolic compounds

“…The sensitivity of the method was evaluated as described by De Sousa et al (2009) where LODs and LOQs were calculated from yintercept standard deviations (S b ) and slopes (a) of calibration curves prepared by adding aliquots of methanol standard mix solution to olive matrix in the concentration ranges (lg g À1 DW) close to LOQs expected for each phenol: oleuropein (6.2-62.0), verbascoside (2.5-25.0), rutin (1.1-11.0), luteolin-7-O-glucoside (3.7-37.0) and quercitrin (1.2-12.5). The parameters were calculated by using equations: LOD = 3.3 Â Sb/a and LOQ = 10 Â Sb/a.…”

Anti-inflammatory and antinociceptive effects of Baccharis dracunculifolia DC (Asteraceae) in different experimental models

“…Detection was performed a 280 nm, and veratraldehyde was used as internal standard. All the phenolic compounds were identified by comparison with the authentic chromatographic standards available at the compounds library of the Pharmacognosy Laboratory of the School of Pharmacy of Ribeirão Preto according to method previously reported (Sousa et al, , 2009). The purity of each standard was estimated by both HPLC and 13 C NMR to be higher than 96%.…”

Barlerisides A and B, new potent superoxide scavenging phenolic glycosides from Barleria acanthoides

“…The combined methanolic extract (300 g) was divided into n-hexane (100 g), EtOAc (60 g), n-BuOH (23 g), and water soluble (95 g) fractions. The n-butanolic fraction was dissolved in water and column-chromatographed over Diaion Compounds 3 and 4 were respectively identified as acteoside and p-hydroxycinnamic acid through comparison of their physical and spectral data with those reported in the literature 5,6 . A solution of 2 (4 mg) in 10% H 2 SO 4 (2 mL) was heated in a boiling water bath for 30 min.…”

“…On further fractionation, the major toxicity was observed in the n-butanolic sub-fraction. This prompted us to carry out studies on this fraction, resulting in the isolation and structural elucidation of two new phenolic glycosides named as barlerisides A (1) and B (2), along with two known compounds acteoside (3) 5 and p-hydroxycinnamic acid (4) 6 . Both compounds 1 and 2 showed significant activity in a superoxide scavenging assay, while they also showed weak inhibitory activity against the enzyme xanthine oxidase.…”