A validated protocol to quantify severity of male urogenital feminization using the MOUSE (Mouse objective urethral severity evaluation)

Abstract

Scoring• Open one of the provided zip folders with 24 randomly assorted photos o The order of these photos correspond with the order of the provided histology • Examine one picture at a time and give each penis a score, record the score in a spreadsheet (e.g., excel) • In preparation for the second scoring attempt re--randomize the set of photos o Keep track of which new numbers correspond with the old numbers • Within a week, but at least 24 hours later) rescore the newly randomized set of photos in a different sheet of the workbook • Unrandomize the second scoring attempt so that data corresponds to histology data o Order the scores by the old identifying numbers • Compile the two scoring attempts into one spreadsheet • Copy and paste the histology ratio column provided in the sheet below into the spreadsheet o 1.

Prenatal exposure to atrazine induces cryptorchidism and hypospadias in F1 male mouse offspring

“…The effect of VCZ on penile masculinization and sexual differentiation resembles that of well‐known AR antagonist flutamide (Gray Jr., Ostby, & Kelce, 1994). A dosage of 150 mg/kg was chosen based on previous studies which suggested that this dose of VCZ may interrupt male sexual differentiation, reproductive function and development without increasing mortality, or other adverse toxic effects (Amato & McCoy, 2016; Gray Jr. et al, 1994; O'Connor, Frame, & Ladics, 2002).…”

“…Disruption of AR in different developmental stages induces different penile anomalies (Zheng, Armfield, & Cohn, 2015). In addition, some environmental endocrine disruptors, such as AR antagonist vinclozolin (VCZ), could induce adverse effects on reproductive system in male rodents (Amato & McCoy, 2016; Hsieh, Breyer, Eisenberg, & Baskin, 2008; Vilela et al, 2007).…”