A Validated HPLC Method With Dual Wavelength Detection for Chlorogenic Acid With an Internal Standard in Plasma and Its Application in Pharmacokinetic Studies in Rats

Wan, Chun-Wai; Lee, Yee-Ki; Kwok, Ching-Yee; Chan, Robbie Yat-Kan; Yu, Peter H.; Chan, Shun‐Wan

doi:10.1080/10826070903524084

Cited by 2 publications

(1 citation statement)

References 26 publications

(24 reference statements)

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“…Chlorogenic acid is a class of the polyphenol group that has antioxidant properties (Bonita, Mandarano, Shuta, & Vinson, ; Esquivel & Jiménez, ). It reaches its blood level peak within 15 min after intravenous administration (Wan et al., ), whereas its maximum level in plasma is 13.33 min with the half‐life of 59.1 min following ip injection (Yuan, Qiao, Xu, Wang, & Li, ). The bioavailability of CGA after oral administration is poor due to the first pass metabolism by the liver and the degradation by normal flora in the digestive tract (Gonthier, Verny, Besson, Remesy, & Scalbert, ).…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic acid ameliorates memory loss and hippocampal cell death after transient global ischemia

Hermawati

Arfian

Mustofa

et al. 2019

Eur J of Neuroscience

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Chlorogenic acid (CGA) is known to have antioxidant potentials, yet the effect of CGA on brain ischemia has not been sufficiently understood. Brain ischemia such as transient global ischemia disrupts many areas of the brain of rats, including the hippocampus. Male Wistar rats were randomly assigned into five groups, that is, sham‐operated (SO), bilateral common carotid occlusion (BCCO), and BCCO+ 15, 30, and 60 mg/kg bw CGA groups (CGA15, CGA30, and CGA60, respectively). Brain ischemia was induced in Wistar rats with BCCO for 20 min followed by intraperitoneal injection of CGA. The rats were examined for the spatial memory in a Morris water maze test on the 3rd day and were euthanized on the 10th day after BCCO. The total number of pyramidal cells was estimated, and the mRNA expressions of Bcl2, Bax, caspase‐3, SOD2, SOD1, GPx, ET‐1, eNOS, CD31, and VEGF‐A were measured. The BCCO group spent less time and distance in the target quadrant than any other group in the spatial memory retention test. The CA1 pyramidal cell numbers in the BCCO and CGA15 groups were lower than in the CGA30 and CGA60 groups. The mRNA expressions of Bcl2, SOD2, and CD31 in the BCCO group were lower than in the CGA15, CGA30, and CGA60 groups. The ET‐1 expression was higher in the BCCO and CGA15 groups than in the SO, CGA30, and CGA60 groups. CGA improves the spatial memory and prevents the CA1 pyramidal cell death after BCCO by increasing Bcl2, SOD2, and CD31 expressions and decreasing ET‐1 expression.

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Section: Discussionmentioning

confidence: 99%

Chlorogenic acid ameliorates memory loss and hippocampal cell death after transient global ischemia

Hermawati

Arfian

Mustofa

et al. 2019

Eur J of Neuroscience

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Bioavailability and metabolism of chlorogenic acids (acyl‐quinic acids) in humans

Clifford

Kerimi

Williamson

2020

Comp Rev Food Sci Food Safe

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Acyl‐quinic acids (chlorogenic acids) are produced by many plants, including fruits, vegetables, and herbal remedies, with coffee and maté particularly rich dietary sources. Epidemiological and intervention studies suggest that they can reduce the risk of developing type 2 diabetes and cardiovascular disease. This review addresses their metabolic handling after oral consumption to provide a mechanistic basis to explain their possible effects on health. Intact acyl‐quinic acids are absorbed only to a small extent in the small intestine, but the cinnamic acids are efficiently absorbed after hydrolysis by either digestive or microbial enzymes in the colon. Metabolism results in phenolic conjugates in the blood and urine, but varying dependent on the acyl‐quinic acid, and subject to significant interperson variability. The balance between hydrogenation and complete β‐oxidation of the cinnamic acids, both by liver and gut microbiota, determines the profile of metabolites. Pharmacokinetic data suggest that some metabolites are bound to human serum albumin and/or sequestered in tissues, and some exhibit biological activity in vitro, consistent with proposed protective action in vivo. Significant gaps in the literature include lack of plasma and urinary data for free‐living individuals, and pharmacokinetic data for groups who consume coffee or maté at regular short intervals. Data are required for cis isomers. There is a critical need for precise urinary biomarkers of consumption of acyl‐quinic acids, accounting for variability in individual metabolism and in beverage composition, thus facilitating better translation of urinary metabolite measurements into accurate coffee consumption data to improve the outcomes of future epidemiological and intervention studies.

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A Validated HPLC Method With Dual Wavelength Detection for Chlorogenic Acid With an Internal Standard in Plasma and Its Application in Pharmacokinetic Studies in Rats

Cited by 2 publications

References 26 publications

Chlorogenic acid ameliorates memory loss and hippocampal cell death after transient global ischemia

Chlorogenic acid ameliorates memory loss and hippocampal cell death after transient global ischemia

Bioavailability and metabolism of chlorogenic acids (acyl‐quinic acids) in humans

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