A Validated HPLC-DAD Method for Simultaneous Determination of Etodolac and Pantoprazole in Rat Plasma

Abstract

A simple, sensitive, and accurate HPLC-DAD method has been developed and validated for the simultaneous determination of pantoprazole and etodolac in rat plasma as a tool for therapeutic drug monitoring. Optimal chromatographic separation of the analytes was achieved on a Waters Symmetry C18 column using a mobile phase that consisted of phosphate buffer pH~4.0 as eluent A and acetonitrile as eluent B in a ratio of A : B, 55 : 45 v/v for 6 min, pumped isocratically at a flow rate of 0.8 mL min−1. The eluted analytes were monitored using photodiode array detector set to quantify samples at 254 nm. The method was linear withr2=0.9999for PTZ andr2=0.9995for ETD at a concentration range of 0.1–15 and 5–50 μgmL−1for PTZ and ETD, respectively. The limits of detection were found to be 0.033 and 0.918 μgmL−1for PTZ and ETD, respectively. The method was statistically validated for linearity, accuracy, precision, and selectivity following the International Conference for Harmonization (ICH) guidelines. The reproducibility of the method was reliable with the intra- and interday precision (% RSD) <7.76% for PTZ and <7.58 % for ETD.

Development and Validation of a New Rp HPLC Analytical Method for the Determination of Etodolac Succinic Acid Co-Crystals in Spiked Rabbit Plasma

“…The separated plasmas were taken in glass tubes. The plasma samples were pooled and 0.5 ml of plasma was taken into each of 10 ml tubes and were spiked with 0.5 ml of ESA standard solution (corresponding to concentration 50,100,150,200,250,300µg/ml) and 0.5 ml of Internal standard (tinidazole RS) corresponding to concentration 200µg/ml was added to each tubes and 0.5 ml acetonitrile was also added to each tube [3].…”

“…In order to fix the wavelength of detection, standard solutions of Etodolac RS and Succinic acid RS and Tinidazole RS were prepared in methanol. Dilutions were made using the mobile phase to get 10μg/ml solutions of Etodolac, Succinic acid and Tinidazole [3].…”

Facile validated HPLC method using photodiode array detector for the combined analysis of etodolac and 5-FU in bulk and tablet dosage form

“…Further, various analytical reports have shown validated quantification methods for the determination of 5-FU alone in samples of biological matrices employing GC/MS [12], HPLC [13,14], hydrophilic interaction liquid chromatography-APCImass spectrometry [15], liquid chromatography tandem mass spectrometry (LC-MS/MS) [16][17][18] and in pharmaceutical dosage forms such as polymeric nanoparticles [19] or ETD alone [20][21]. Furthermore, Table 1 summarizes the comparative data of analytical methods/chromatographic studies in chronological order carried out in the last decade on individual analytes 5-FU or ETD or in combination with the agents of other therapeutic class /analytes from the literature [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]. This comparative analysis offer distinct understanding of the sensitivity (in terms of LOD/LLOQ) of the analytical method using PDA detector conducted in the present study in comparison to other methods for the analysis of 5-FU or ETD or in combination with agents of other therapeutic class.…”

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“…Various analytical methods used for the determination of this drug in the body fluid and pharmaceutical preparations. These include Spectrofluorometric method 7, 8 , Gas Chromatography GC 9 , Sequential injection analysis 10 , High-Performance Method HPLC [11][12][13][14][15][16][17] , High-Performance Thin Layer Chromatography HTLC 18 , Capillary electrophoresis 19,20 , Voltammetry 21 and UV-spectro-photometric [21][22][23][24][25][26][27][28][29][30] . The current paper reports a simple, sensitive spectrophotometric method for determination of Etodolac via the reaction of the drug with ferric ion Fe 3+ to form the corresponding ferrous ion Fe 2+ and subsequent reaction with potassium hexacyanoferrate to form a colored compound that is absorbed in 700 nm.…”