A validated high‐performance thin‐layer chromatography method for the identification and simultaneous quantification of six markers from Platanus orientalis and their cytotoxic profiles against skin cancer cell lines

Abstract

Betulinic acid (1), betulinic acid-3-acetate (2), 3-acetylbetulinaldehyde (3), oleanolic acid-3-acetate (4), 3-β-hydroxy-28,19-β-olenolide (5), and β-sitosterol (6) were isolated from Platanus orientalis and a high-performance thin-layer chromatography method was developed for their simultaneous quantification. The markers were first derivatized on the chromatogram with ceric ammonium sulfate and then high-performance thin-layer chromatography densitometry was carried out. Chromatographic separation of these markers was carried out on silica gel 60 plates using a ternary solvent system n-hexane/toluene/acetone (6:3.5:1 v/v/v) as a mobile phase. For marker 1, a deuterium (D2) lamp and wavelength of 420 nm was used. A tungsten (W) lamp was used for markers 2 and 3 at 550 nm and for 4-6 at 500 nm. The method was validated for accuracy, precision, LOD, and LOQ. All calibration curves showed a good linear relationship (r > 0.9919). The precision evaluated by an intra- and interday study showed RSDs < 2.51% and accuracy validation recovery between 95.54 and 99.33% with RSDs < 1.55%. The successful application of the validated method showed 1 as the most abundant component (4.63%) and 5 (0.017%) the least. The markers displayed a significant cytotoxic effect against human keratinocyte, mouse melanoma, and human skin epithelial carcinoma cancer cells by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Risk assessment, dissipation behavior and persistence of quinalphos in/on green pea by gas chromatography with electron capture detector

“…The accuracy was then calculated from the test results as the percentage of recovery and the results have been summed up in Table . The control samples of green pea were spiked at 0.09, 0.20, and 0.60 mg/kg, respectively, and percent recoveries (Equation ) of quinalphos in green pea were found to be consistent and more than 73% (Table ) indicating high accuracy of the developed method. The recovery (%) was calculated by Equation .…”

Isolation, cytotoxic evaluation, and simultaneous quantification of eight bioactive secondary metabolites fromCicer microphyllumby high-performance thin-layer chromatography

“…The chemical constituents of the plant were identified with potent cytotoxic potential through in vitro assay. The presence of the bioactive compounds particularly luteolin (8) in the plant opens new avenues with regard to drug development. The existence of Pratensein (6) in major quantities in the plant is also important because of its known antioxidant, anti-inflammatory, anti-mutagenic, and anti-obesity activities.…”

“…Phytochemical investigation of DCM/MeOH (1:1) extract yielded eight compounds (1) viz Stigmasterol (1), Oleanolic acid-3-acetate (2), Oleanolic acid (3), Biochanin A (4), Genistein (5), Pratensein (6), Chrysoeriol (7), and Luteolin (8). All the compounds were well characterized using spectroscopic techniques (spectra are provided in the Supporting Information) and reported for first time from this plant.…”

Bark Extractives and Suberin Monomers from Arbutus andrachne and Platanus orientalis

“…It is seen in the parks and landscapes as an ornamental plant (Khan et al 2013). The phenolic composition of its leaves and buds and the effects of environmental stress have been studied, but there is limited knowledge of the chemical composition of P. orientalis bark.…”