A Validated Bioanalytical Method for Quantification of Ziprasidone in Rabbit Plasma by LC-MS/MS: Application to a Pharmacokinetic Study

Structural analysis of anti-retroviral drug raltegravir and its potential impurity C: investigation of solubility and stability

“…In order to investigate the stability advantage that anhydrous RLT form A has over its K salt, the RLT solid forms were treated with 0.1 N HCl and their degradation was monitored at 45 and 90 min using HPLC. Using two previously reported HPLC methods as reference, 20,21 we developed a new HPLC process for the analysis of the RLT samples and compared it with the standard RLT. The results indicated that the RLT K salt exhibited 10.5% and 19.9% degradation after 45 and 90 min, respectively, whereas the RLT hydrate exhibited 10.8% and 12% degradation after the same time intervals.…”

“…Using two previously reported HPLC methods as reference, 20,21 we developed a new HPLC process for the analysis of the RLT samples and compared it with the standard RLT. The results indicated that the RLT K salt exhibited 10.5% and 19.9% degradation after 45 and 90 min, respectively, whereas the RLT hydrate exhibited 10.8% and 12% degradation after the same time intervals.…”

“…16,17 There are several reports on improved synthesis of RLT and its pure impurities for analytical purposes. 5,[18][19][20][21] The synthesis of highly pure RLT impurities as a reference standard in a low economic process is highly challenging for industries. Here we report another suitable method of synthesis of RLT impurity C via hydrolysis of an oxadiazole moiety in isopropanol or ethyl acetate using reflux conditions (Scheme 1).…”

Comparative study of performances of UHPLC-MS/MS and HPLC/UV methods for analysis of ziprasidone and its main impurities

“…Several methods have been developed for therapeutic monitoring of ziprasidone and for pharmacokinetic studies in biological (both human and animal) samples such, as voltammetry [6], high performance liquid chromatography (HPLC) with the ultraviolet (UV) [7][8][9] and fluorescence detection [10], and HPLC with monitoring of radioactivity for investigation of the primary oxidative metabolites [11]. Determination of ziprasidone by means of HPLC with the single and tandem MS detection was performed mainly in biological samples, either alone, or in combination with the other CNS drugs [12][13][14][15][16][17][18][19][20]. Quantitative determination of ziprasidone in raw materials and pharmaceuticals was performed by spectrophotometry [21], high performance thin layer chromatography (HPTLC) [22], thin-layer chromatography (TLC) [23], capillary electrophoresis (CE) [24], HPLC with UV detection [25][26][27] and ultra high performance liquid chromatography (UHPLC) with UV detection [28].…”