A Vacuolar Phytosiderophore Transporter Alters Iron and Zinc Accumulation in Polished Rice Grains

Che, Jianfei; Yokosho, Kengo; Yamaji, Naoki; Feng, Jian

doi:10.1104/pp.19.00598

Cited by 68 publications

(56 citation statements)

References 61 publications

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“…of OsVMT, a vacuolar transporter for phytosiderophore enhanced both Fe and Zn accumulation in polished rice grain through increasing solubilization of these metals deposited in the node (Che et al, 2019). In this study, knockout of OsVIT2 also resulted in a significant increase in Fe concentration in the grain without yield penalty (Fig.…”

Section: Transporters Expressed In the Node I As Targets For Iron Biosupporting

confidence: 55%

Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

2021

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Iron (Fe) from rice grains is an important source of dietary intake; however, the molecular mechanisms responsible for loading of Fe to the grains are poorly understood. We functionally characterized a vacuolar iron transporter gene, OsVIT2 in terms of expression pattern, cellular localization, and mutant phenotypes. OsVIT2 was expressed in the parenchyma cell bridges of nodes, in the mestome sheath of leaf sheath and aleurone of the caryopsis. Mutation of OsVIT2 resulted in decreased Fe distribution to the leaf sheath, nodes, and aleurone, but increased Fe to the leaf blade and grains. Furthermore, Fe was heavily deposited in the parenchyma cell bridges, mestome sheath and aleurone in the wild-type rice, but this accumulation was decreased in the knockout lines. Conversely, heavier deposition of Fe was observed in the embryo and endosperm of the grains of knockout lines compared with the wild-type rice, resulting in increased Fe accumulation in the polished rice without yield penalty. These results indicate that OsVIT2 is involved in the distribution of Fe to the grains through sequestering Fe into vacuoles in mestome sheath, nodes, and aleurone layer and that knockout of this gene provides a potential way for Fe biofortification without yield penalty.

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Section: Transporters Expressed In the Node I As Targets For Iron Biosupporting

confidence: 55%

Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

2021

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“…OsZIP9 knockout lines were generated by using CRISPR/Cas9 using the plant expression vector of Cas9 (pU6gRNA) and single-guide RNA expression vector (pZDgRNA_Cas9ver.2_HPT) as described in Che et al (2019). Twenty bases upstream of the PAM motif were selected as candidate target sequences (Supplemental Fig.…”

Section: Generation Of Transgenic Rice Linesmentioning

confidence: 99%

The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions

et al. 2020

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Zinc (Zn) is an important essential micronutrient for plants and humans; however, the exact transporter responsible for root zinc uptake from soil has not been identified. Here, we found that OsZIP9, a member of the ZRT-IRT-related protein, is involved in Zn uptake in rice (Oryza sativa) under Zn-limited conditions. OsZIP9 was mainly localized to the plasma membrane and showed transport activity for Zn in yeast (Saccharomyces cerevisiae). Expression pattern analysis showed that OsZIP9 was mainly expressed in the roots throughout all growth stages and its expression was upregulated by Zn-deficiency. Furthermore, OsZIP9 was expressed in the exodermis and endodermis of root mature regions. For plants grown in a hydroponic solution with low Zn concentration, knockout of OsZIP9 significantly reduced plant growth, which was accompanied by decreased Zn concentrations in both the root and shoot. However, plant growth and Zn accumulation did not differ between knockout lines and wild-type rice under Zn-sufficient conditions. When grown in soil, Zn concentrations in the shoots and grains of knockout lines were decreased to half of wild-type rice, whereas the concentrations of other mineral nutrients were not altered. A shortterm kinetic experiment with stable isotope 67 Zn showed that 67 Zn uptake in knockout lines was much lower than that in wildtype rice. Combined, these results indicate that OsZIP9 localized at the root exodermis and endodermis functions as an influx transporter of Zn and contributes to Zn uptake under Zn-limited conditions in rice.

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“…It was found that introduction of rice Mn-NA transporter with barley NA ++ synthase gene and soybean Ferritin gene resulted in 4.4-fold Fe increase and 1.6-fold Zn increase in transgenic rice grain ( Masuda et al, 2012 ). Che et al (2019) reported that OsVMT knockout resulted in increased Zn and Fe level in rice grain. Yamaji et al (2017) reported that novel plasma membrane-localized transporter gene (OsSPDT) knockout resulted in ∼20% decrease of phytic acid in rice grains.…”

Section: Manipulation Of Transportersmentioning

confidence: 99%

Genetic Manipulation for Improved Nutritional Quality in Rice

2020

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Food with higher nutritional value is always desired for human health. Rice is the prime staple food in more than thirty developing countries, providing at least 20% of dietary protein, 3% of dietary fat and other essential nutrients. Several factors influence the nutrient content of rice which includes agricultural practices, post-harvest processing, cultivar type as well as manipulations followed by selection through breeding and genetic means. In addition to mutation breeding, genetic engineering approach also contributed significantly for the generation of nutrition added varieties of rice in the last decade or so. In the present review, we summarize the research update on improving the nutritional characteristics of rice by using genetic engineering and mutation breeding approach. We also compare the conventional breeding techniques of rice with modern molecular breeding techniques toward the generation of nutritionally improved rice variety as compared to other cereals in areas of micronutrients and availability of essential nutrients such as folate and iron. In addition to biofortification, our focus will be on the efforts to generate low phytate in seeds, increase in essential fatty acids or addition of vitamins (as in golden rice) all leading to the achievements in rice nutrition science. The superiority of biotechnology over conventional breeding being already established, it is essential to ascertain that there are no serious negative agronomic consequences for consumers with any difference in grain size or color or texture, when a nutritionally improved variety of rice is generated through genetic engineering technology.

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A Vacuolar Phytosiderophore Transporter Alters Iron and Zinc Accumulation in Polished Rice Grains

Cited by 68 publications

References 61 publications

Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

Role of a vacuolar iron transporter OsVIT2 in the distribution of iron to rice grains

The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions

Genetic Manipulation for Improved Nutritional Quality in Rice

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