A UPLC–MS/MS method for analysis of vancomycin in human cerebrospinal fluid and comparison with the chemiluminescence immunoassay

Abstract

Vancomycin (VCM) is clinically used in treating patients with postoperative intracranial infections. The cerebrospinal fluid (CSF) concentration of VCM varies greatly among patients. To guide the dosage regimens, monitoring of VCM in CSF is needed. However a method for analysis of VCM in human CSF is lacking. An ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for analysis of VCM in human CSF, and the agreement of UPLC-MS/MS and chemiluminescence immunoassay (CLIA) in the analysis of CSF VCM was evaluated. The ion transitions were m/z 725.5 > 144.1 for VCM and m/z 455.2 > 308.2 for methotrexate (internal standard). The agreement between UPLC-MS/MS and CLIA was evaluated by Bland-Altman plot in 179 samples. The calibration range of the UPLC-MS/MS method was 1-400 mg/L. The inaccuracy and imprecision were -0.69-10.80% and <4.95%. The internal standard normalized recovery and matrix factor were 86.14-99.31 and 85.84-92.07%, respectively. The measurements of CLIA and UPLC-MS/MS were strongly correlated (r > 0.98). The 95% limit of agreement of the ratio of CLIA to UPLC-MS/MS was 61.66-107.40%. Further studies are warranted to confirm the results.

Chemical Biology Toolsets for Drug Discovery and Target Identification

“…The treatment concentration of vanomycin greatly varies among the patients. UPLC-MS technique was developed and used for the analysis of VCM in human cerebrospinal fluid [38].…”

Measurement of methotrexate in human cerebrospinal fluid using a chemiluminescence immunoassay intended for serum and plasma matrices

“…[9][10][11] In the present study, we aimed to construct an environment for measuring the concentration of MTX in the CSF using a CLIA method following past reports. 12,13 We measured the concentration of MTX in CSF using a high-performance liquid chromatography (HPLC) method, intended for CSF, and a CLIA method, intended for serum and plasma matrices. We also determined the differences in the results obtained using the two methods.…”

Studies on the metabolism and degradation of vancomycin in simulated in vitro and aquatic environment by UHPLC-Triple-TOF-MS/MS

“…As a commonly used clinical drug, vancomycin is one of the PPCPs that is of significant concern. Among all the glycopeptide antibiotics in clinical use, vancomycin is the most commonly used to treat serious infections like endocarditis, pneumonia and meningitis, caused by vancomycin-susceptible Gram-positive organisms, such as methicillin-resistant Staphylococcus aureus (MRSA) 7 , 8 . In Italy and Germany, treated urban discharges can be an entry route of vancomycin into surface water, because wastewater treatment processes are not completely effective in removing vancomycin 9 .…”