A UPLC-MRM-MS method for comprehensive profiling of Amadori compound-modified phosphatidylethanolamines in human plasma

He, Xia; Li, Zhucui; Zhang, Qibin

doi:10.1007/s00216-020-03012-w

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…The distribution and diversity of protein modifications by electrophiles, the “adductome”, is complex and likely to be characteristic for particular diseases . The sensitivity of mass spectrometric techniques, exemplified by the work of He et al, also revealed a similar complexity for lipid modifications by glycation. While the levels of these modifications may not have a significant physiological effect, they may offer up the potential for early detection of disease conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The most abundant species detected, with plasma concentrations >5 nM, were the plasmalogens PE P-16:0/20:4, PE P-18:0/20:4, and PE P-18:1/20:4 and the lipids PE 18:0/20:4, PE 18:0/18:2, and PE16:0/22:6. By comparison with the corresponding unmodified PE lipids in the sample, the glycation rate was estimated at approximately 0.07%. , …”

Section: Oxidationmentioning

confidence: 99%

“…By comparison with the corresponding unmodified PE lipids in the sample, the glycation rate was estimated at approximately 0.07%. 441,442 Given the role of mitochondria in respiration and the generation of ROS, it is unsurprising that attention had been given to the oxidative stability of mitochondrial lipids. Particular attention has focused on PE lipids and cardiolipins (CDLs), which are major components of mitochondrial membranes, and their reaction with perhydroxyl radicals formed in the proximity of the inner membrane from the superoxide radical.…”

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confidence: 99%

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The Chemical Reactivity of Membrane Lipids

Duché,

Sanderson

2024

Chem. Rev.

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It is well-known that aqueous dispersions of phospholipids spontaneously assemble into bilayer structures. These structures have numerous applications across chemistry and materials science and form the fundamental structural unit of the biological membrane. The particular environment of the lipid bilayer, with a water-poor low dielectric core surrounded by a more polar and better hydrated interfacial region, gives the membrane particular biophysical and physicochemical properties and presents a unique environment for chemical reactions to occur. Many different types of molecule spanning a range of sizes, from dissolved gases through small organics to proteins, are able to interact with membranes and promote chemical changes to lipids that subsequently affect the physicochemical properties of the bilayer. This Review describes the chemical reactivity exhibited by lipids in their membrane form, with an emphasis on conditions where the lipids are well hydrated in the form of bilayers. Key topics include the following: lytic reactions of glyceryl esters, including hydrolysis, aminolysis, and transesterification; oxidation reactions of alkenes in unsaturated fatty acids and sterols, including autoxidation and oxidation by singlet oxygen; reactivity of headgroups, particularly with reactive carbonyl species; and E/Z isomerization of alkenes. The consequences of reactivity for biological activity and biophysical properties are also discussed. CONTENTS

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Section: Discussionmentioning

confidence: 99%

Section: Oxidationmentioning

confidence: 99%

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confidence: 99%

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The Chemical Reactivity of Membrane Lipids

Duché,

Sanderson

2024

Chem. Rev.

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“…The relative abundance of Phosphoethanolamine was reduced by CO in HFD mice in our study. Phosphoethanolamine is an abundant signaling phospholipid and participated in the mechanism of glucose, while exhibiting a negative correlation with TC but was positively corrected with TG [45]. Further experiments are needed to demonstrate this opposite regulatory mechanism.…”

Section: Discussionmentioning

confidence: 99%

Camellia (Camellia oleifera Abel.) Seed Oil Regulating of Metabolic Phenotype and Alleviates Dyslipidemia in High Fat-Fed Mice through Serum Branch-Chain Amino Acids

Gao

et al. 2022

Nutrients

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Camellia (Camellia oleifera Abel.) seed oil (CO) has been shown to effectively reduce the blood lipid level of its host due to its fatty acid content, but the specific molecular mechanism associated with the metabolic phenotype after digestion is not clear. Here, we further investigated the relationship between branched-chain amino acids (BCAA) and the metabolic phenotype that may exhibit the anti-dyslipidemia effect of CO on mice fed a high-fat diet for 30 day C57BL/6J male mice were allocated to three groups: the control group (Cont), the high-fat feed group (HFD), and a high-fat feed group with CO treatment (CO). A serum sample was collected to detect lipid biomarkers and BCAA concentration. Notably, Low-density lipoprotein (LDL), Total Cholesterol (TC), and Triglycerides (TG) showed a significant decrease, whereas High-density lipoprotein (HDL) increased in CO mice but not in the HFD group. The concentration of Isoleucine (Ile), leucine (Leu), and valine (Val) was similar between the Cont and CO groups compared with the HFD group, exhibiting an inhibition induced by CO in mice fed with a high-fat diet. A metabolic phenotype from serum examined by non-targeted metabolite analysis using UHPLC/MS showed most metabolites exhibited lipid and BCAA metabolism. The results indicated that CO treatment notably regulated the metabolism of arachidonic acid and steroid biosynthesis in response to HFD-induced dyslipidemia. In addition, the expression of PPARγ genes that correlated with the BCAA and serum lipid biomarkers were compared, and significant inhibition was noticed, which might lead to the potential exposure of the anti-dyslipidemia mechanism of CO in HFD-fed mice. In conclusion, the expression of PPARγ genes, serum lipid level, BCAA concentration, and the metabolic phenotype was significantly positive in correlation with a high-fat diet, whereas oral CO improved the biomarkers and metabolism of some specific serum metabolites in HFD-fed mice.

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What if using certified reference materials (CRMs) was a requirement to publish in analytical/bioanalytical chemistry journals?

Wise

2022

Anal Bioanal Chem

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A UPLC-MRM-MS method for comprehensive profiling of Amadori compound-modified phosphatidylethanolamines in human plasma

Cited by 5 publications

References 19 publications

The Chemical Reactivity of Membrane Lipids

The Chemical Reactivity of Membrane Lipids

Camellia (Camellia oleifera Abel.) Seed Oil Regulating of Metabolic Phenotype and Alleviates Dyslipidemia in High Fat-Fed Mice through Serum Branch-Chain Amino Acids

What if using certified reference materials (CRMs) was a requirement to publish in analytical/bioanalytical chemistry journals?

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