A universal ratiometric method for Micro-RNA detection based on the ratio of electrochemical/electrochemiluminescence signal, and toehold-mediated strand displacement amplification

Niazi, Mohammad; Azizi, Ava; Khajavi, Zeynab; Sheikh, Milad; Taheri, Salman; Radfar, Sasan; Alizadeh, Abdolhamid; Ghanbari, Reza

doi:10.1016/j.aca.2023.341119

Cited by 8 publications

(1 citation statement)

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“…Moreover, since it is difficult for a single electrochemical method to accurately detect and specifically identify analytes in complex biological samples, in order to overcome this shortcoming, electrochemical signals are often used as an internal reference to correct the experimental error caused by uneven modification of the probe on the electrode surface and improve reproducibility. 113 For the first time, Niazi et al 114 attached a common internal molecule of a luminescent group (Ru(phen) 3 2+ ) to Ti 3 C 2 -Au, generating a unique nanocomposite (Ti 3 C 2 -Au-Ru) that can generate a stable ECL signal (Fig. 12B).…”

Section: Multi-modal Nanoprobes Enabled Multi-modal Biosensorsmentioning

confidence: 99%

Multi-modal nanoprobe-enabled biosensing platforms: a critical review

Li,

Zhang,

et al. 2024

Nanoscale

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Section: Multi-modal Nanoprobes Enabled Multi-modal Biosensorsmentioning

confidence: 99%

Multi-modal nanoprobe-enabled biosensing platforms: a critical review

Li,

Zhang,

et al. 2024

Nanoscale

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Sensitive and Specific Y-Shaped Ratio Biosensor for Detecting Serum miR-18a: Potential Early Scanning Tool for Non-Small Cell Lung Cancer

Yu,

Wang,

et al. 2024

J. Anal. Test.

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AbstractmiR-18a has been identified as a significantly expressed microRNA (miRNA) in non-small cell lung cancer (NSCLC) and plays a vital role in cancer cell transformation, metastasis, and carcinogenesis. Herein, a pair of binary probes from numerous probe pairs based on single nucleotide polymorphism analyses of miR-18a and miR-18b was first designed and screened to develop a Y-shaped ratio biosensor for accurate detection of serum miR-18a in NSCLC. The special structure of the binary probes combined with the hairpin showed strong specificity for miR-18a, which was confirmed by polyacrylamide gel electrophoresis assay and square wave voltammetry assay. Furthermore, it is beneficial to immobilize single-stranded DNA (ssDNA) probes due to the large specific surface area of nanoporous gold, thereby improving the sensitivity of the biosensor. The Y-shaped ratio biosensor exhibited a wide detection range and can quantify the concentration of miR-18a in the range of 10 fmol/L–100 pmol/L, with a limit of detection of 0.211 fmol/L (S/N = 3). Moreover, it exhibits excellent detection capabilities in serum samples since the biosensor showed a high selectivity toward the coexistence of miR-18a and miR-18b. Therefore, the prepared Y-shaped ratio biosensor is a highly sensitive and specific miR-18a detection tool, capable of identifying microscopic amounts of miR-18a in serum samples, providing great potential for early screening of NSCLC.

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