A universal primer multiplex PCR method for typing of toxinogenic Pseudomonas aeruginosa

Development of loop-mediated isothermal amplification assays for genotyping of Type III Secretion System in Pseudomonas aeruginosa

“…In our previous study, the occurrence of various virulence genes from P. aeruginosa was investigated with universal‐primer multiplex PCR (Shi et al . 2012a). Among these genes, exoU and exoS have great contribution to pathogenesis and can be used to judge the virulence of P. aeruginosa strains.…”

“…These limits are lower than those observed with novel universal‐primer multiplex PCR for P. aeruginosa with a detection limit of 1 pg (Shi et al . 2012a) and conventional PCR with a detection limit of 10 pg (Jeong et al . ; Zhao et al .…”

“…PCR amplifications with primers for the ecfX internal control gene were performed as is described in our previous study (Shi et al . 2012a). P. aeruginosa isolations were used in the LAMP and PCR experiments.…”

“…; Shaver and Hauser ; Shi et al . 2012a). Therefore, it is vitally necessary to detect the presence of exoS and exoU to judge virulence of P. aeruginosa in outbreak situations or in routine surveillance studies.…”

“…; Shi et al . 2012a); however, disadvantages for PCR (use of expensive thermal cycling device, low detection limits and time‐consuming product detection) can pose obstacles for its application.…”