A universal platform for sensitive and selective colorimetric DNA detection based on Exo III assisted signal amplification

Cui, Liang; Ke, Guoliang; Zhang, Wei Yun; Yang, Chaoyong

doi:10.1016/j.bios.2010.11.005

Cited by 62 publications

(37 citation statements)

References 46 publications

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“…In recent years, enzymes have been widely applied for the development of amplified colorimetric sensors to detect DNA because the mechanisms of various enzymes involve nucleic acids as substrates [194][195][196][197][198][199][200][201]. Liu et al developed an approach to enhance the sensitivity of a DNA sensor by using nicking endonuclease-assisted nanoparticle amplification (NEANA) [194].…”

Section: Increased Aggregate Size: Enzyme-guided Aggregate Formationmentioning

confidence: 99%

Strategies for enhancing the sensitivity of plasmonic nanosensors

et al. 2015

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Based on the localized surface plasmon resonance (LSPR) of metallic nanoparticles, plasmonic nanosensors have emerged as a powerful tool for biosensing applications. Many detection schemes have been developed and the field is rapidly growing to incorporate new methodologies and applications. Amidst all the ongoing research efforts, one common factor remains a key driving force: continued improvement of high-sensitivity detection. Although there are many excellent reviews available that describe the general progress of LSPR-based plasmonic biosensors, there has been limited attention to strategies for improving the sensitivity of plasmonic nanosensors. Recognizing the importance of this subject, this review highlights recent progress on different strategies used for improving the sensitivity of plasmonic nanosensors. These strategies are classified into the following three categories based on their different sensing mechanisms: (1) sensing based on target-induced local refractive index changes, (2) colorimetric sensing based on LSPR coupling, and (3) amplification of detection sensitivity based on nanoparticle growth. The basic principles and cutting-edge examples are provided for each kind of strategy, collectively forming a unifying framework to view the latest attempts to improve the sensitivity of nanoplasmonic sensors. Future trends for the fabrication of improved plasmonic nanosensors are also discussed.

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Section: Increased Aggregate Size: Enzyme-guided Aggregate Formationmentioning

confidence: 99%

Strategies for enhancing the sensitivity of plasmonic nanosensors

et al. 2015

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“…For example, the limit of colorimetric detection for a short DNA sequence by conventional sandwich assays was about 10 nM, which was much higher than the detection limit (typically below 1 nM) required in many diagnostic assays (Zhao et al, 2008a;Rosi et al, 2005). To meet these challenges and significantly improve the detection sensitivity, different types of enzyme-modified AuNPs colorimetric assay have been utilized for signal amplification (Xu et al, 2009Yang and Gao, 2014;Cui et al, 2011;Zhao et al, 2008b). However, the enzymes needed in these approaches brought about the complexity of the experimental system and increased the detection cost which limited the application of these techniques.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive visual detection of DNA with tunable dynamic range by using unmodified gold nanoparticles and target catalyzed hairpin assembly amplification

Yun

Jiang

Cai

et al. 2016

Biosensors and Bioelectronics

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“…[3][4][5] Integration of AuNP-based color detection with isothermal amplication has been reported for DNA detection, aiming to achieve POC application with clinically relevant high sensitivity. 3,6 For example, by coupling Exonuclease III (ExoIII) recycling cleavage reaction into AuNPs colorimetric assay, one can detect SNP in a 32 nucleotide (nt) KRAS with 20 pM LOD at 37 C, 7 and in a 33 nt DNA with a 2 nM LOD at room temperature (RT). 8 Enzyme-free hybridization chain reaction has also been developed with colorimetric AuNP assembly to detect Ebola all four virus subtypes DNA biomarker (24 nt).…”

Section: Introductionmentioning

confidence: 99%

“…9 Most of these reports are for short DNA biomarkers (20-30 bp). [7][8][9] It has been very challenging for SNP detection in clinical ctDNA (average of 150 bp length, more diverse conformation and secondary structure in need of assay consideration) with high detection sensitivity, and more importantly in the complicated high background interference. These are, however, essential for circulating DNA biomarker detection for diagnosis and prognosis.…”

Section: Introductionmentioning

confidence: 99%