A Universal, Multi-Modality Localization System for Animal Radiosurgery

Solberg, T.D.; Salles, Antonio A. F. De; Hovda, David A.; Holly, F. Eugene

doi:10.1007/978-3-7091-9371-6_6

Cited by 10 publications

(8 citation statements)

References 5 publications

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“…The use of clinical radiosurgery equipment in pre-clinical irradiation has been described previously29,30. Dose equivalence was calculated from the linear-quadratic model using a tumor α/β ratio of 10.…”

Section: Resultsmentioning

confidence: 99%

Correlation Between Tumor Growth Delay and Expression of Cancer and Host VEGF, VEGFR2, and Osteopontin in Response to Radiotherapy

Solberg

Nearman

Mullins

et al. 2008

International Journal of Radiation Oncology*Biology*Physics

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Purpose-to determine late effects of radiotherapy on the VEGF, VEGFR2 and OPN expression in cancer and stromal cells.Methods and Materials-LS174T xenografted athymic mice were used as a tumor model. Radiation was delivered in two equivalent fractionation schemes: 5×7 Gy and 1×20 Gy, the latter at two dose rates.Results-Tumor growth arrest was similar in all treatment groups with the exception of a better response of small-sized tumors in the 5×7 Gy group. Host VEGF and OPN levels were directly proportional to tumor doubling time (T D ) and were independent of the fractionation scheme. Host and cancer cell VEGFR2 levels in tumor were also directly related to the tumor response to radiotherapy.Conclusion-Upregulated VEGFR2 in cancer cells suggest paracrine signaling in the VEGFR2 pathway of cancer cells as the factor contributing to the radiotherapy failure. The transient activation of the host VEGF/VEGFR2 pathway in tumor supports the model of angiogenic regeneration and suggests that radiation-induced upregulation of VEGF, VEGFR2, and downstream proteins may contribute to the failure of radiotherapy by escalating the rate of vascular repair. Co-expression of host OPN and VEGF, two factors closely associated with angiogenesis, indicate that OPN can serve as a surrogate marker of the tumor recovery after radiotherapy. Taken together these results strongly support the notion that to achieve optimal therapeutic outcome, the scheduling of radiation and antiangiogenic therapies will require patient-specific post-treatment monitoring of the VEGF/VEGFR2 pathway and that tumor-associated OPN can serve as an indicator of the tumor regrowth.

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Section: Resultsmentioning

confidence: 99%

Correlation Between Tumor Growth Delay and Expression of Cancer and Host VEGF, VEGFR2, and Osteopontin in Response to Radiotherapy

Solberg

Nearman

Mullins

et al. 2008

International Journal of Radiation Oncology*Biology*Physics

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“…Each animal was initially anesthetized using ketamine (10 mg/kg, intramuscularly) followed by the administration of atropine sulfate (0.04 mg/kg, intramuscularly), maintenance doses of anesthetics (ketamine 20 mg/kg/hr and midazolam 0.2-0.4 mg/kg/hr, via the saphenous vein), and intubation. Before the imaging studies, an MR imaging-compatible stereotactic frame 12,52 was secured with 4 pins to the animal's zygomatic and occipital bones after local lidocaine injections. The stereotactic frame was designed to precisely fit the commercially available human stereotactic device CRW (Radionics, Inc.).…”

Section: Magnetic Resonance Imaging and Surgery For Vmh Dbsmentioning

confidence: 99%

Modulation of food intake following deep brain stimulation of the ventromedial hypothalamus in the vervet monkey

Laćan¹,

Salles

Gorgulho³

et al. 2008

JNS

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“…Radiation dose characteristics are similar to kV and orthovoltage X-ray irradiators and must be characterised, particularly for the small fields used for irradiation. Focal stereotactic irradiation of small animals using a specialised clinical gamma unit (Kondziolka et al 1992, Lunsford et al 1990, DesRosiers et al 2003, Wiant et al 2009) or linear accelerator (Spiegelmann et al 1993, Solberg et al 1994, Blatt et al 1994) has also been performed.…”

Section: Introductionmentioning

confidence: 99%

Specific issues in small animal dosimetry and irradiator calibration

Yoshizumi¹,

Brady

Robbins

et al. 2011

International Journal of Radiation Biology

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Purpose In response to the increased risk of radiological terrorist attack, a network of Centers for Medical Countermeasures against Radiation (CMCR) has been established in the United States, focusing on evaluating animal model responses to uniform, relatively homogenous whole- or partial-body radiation exposures at relatively high dose rates. The success of such studies is dependent not only on robust animal models but on accurate and reproducible dosimetry within and across CMCR. To address this issue, the Education and Training Core of the Duke University School of Medicine CMCR organised a one-day workshop on small animal dosimetry. Topics included accuracy in animal dosimetry accuracy, characteristics and differences of cesium-137 and X-ray irradiators, methods for dose measurement, and design of experimental irradiation geometries for uniform dose distributions. This paper summarises the information presented and discussed. Conclusions Without ensuring accurate and reproducible dosimetry the development and assessment of the efficacy of putative countermeasures will not prove successful. Radiation physics support is needed, but is often the weakest link in the small animal dosimetry chain. We recommend: (i) A user training program for new irradiator users, (ii) subsequent training updates, and (iii) the establishment of a national small animal dosimetry center for all CMCR members.

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A Universal, Multi-Modality Localization System for Animal Radiosurgery

Cited by 10 publications

References 5 publications

Correlation Between Tumor Growth Delay and Expression of Cancer and Host VEGF, VEGFR2, and Osteopontin in Response to Radiotherapy

Correlation Between Tumor Growth Delay and Expression of Cancer and Host VEGF, VEGFR2, and Osteopontin in Response to Radiotherapy

Modulation of food intake following deep brain stimulation of the ventromedial hypothalamus in the vervet monkey

Specific issues in small animal dosimetry and irradiator calibration

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