A universal epitope-based influenza vaccine and its efficacy against H5N1

Adar, Yaakov; Singer, Yossi; Levi, Raphael; Tzehoval, Esther; Perk, Shimon; Banet-Noach, Caroline; Nagar, Sagit; Arnon, Ruth; Ben-Yedidia, Tamar

doi:10.1016/j.vaccine.2009.02.011

Cited by 88 publications

(65 citation statements)

References 49 publications

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“…The second was the growing evidence that flagellin can be a potent adjuvant (28). In both cases, it is also clear that antigen delivery and TLR activation must be targeted to the same antigen-presenting cell as a fusion product or coformulation (1,16,25,31). We employed the unique approach of displaying both antigen and adjuvant simultaneously on the bacterial cell surface using FliC as the adjuvant.…”

Section: Discussionmentioning

confidence: 99%

Construction and Immunological Evaluation of Dual Cell Surface Display of HIV-1 Gag and Salmonella enterica Serovar Typhimurium FliC in Lactobacillus acidophilus for Vaccine Delivery

Kajikawa

Zhang

Long

et al. 2012

Clin Vaccine Immunol

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ABSTRACTOral vaccines that elicit a mucosal immune response may be effective against human immunodeficiency virus type 1 (HIV-1) because its transmission occurs mainly at the mucosa. The aim of this study was to construct recombinantLactobacillusfor oral delivery of oral vaccines against HIV-1 and to evaluate their immunogenicity. A recombinantLactobacillus acidophilusstrain expressing the HIV-1 Gag on the bacterial cell surface was established by fusion with the signal peptide and anchor motif of a mucus binding protein (Mub) fromL. acidophiluswith or without coexpression ofSalmonella entericaserovar Typhimurium flagellin (FliC) fused to a different Mub signal peptide and anchor. Using HEK293 cells engineered to express Toll-like receptor 5 (TLR5), the biological activity of FliC on the bacterial cell surfaces was determined. The surface-exposed flagellin retained its TLR5-stimulating activity, suggesting that the recombinant strain with Gag and FliC dual display might provide a different immunopotency than the strain expressing only Gag. The immunological properties of the recombinant strains were assessed by coculture with human myeloid dendritic cells (DCs). The heterologous antigens on the cell surface affected maturation and cytokine responses of DCs. Acquired immune responses were also investigated by intragastric immunization of mice. The enzyme-linked immunosorbent spot assay showed induction of gamma interferon-producing cells at local mucosa after immunization of mice with the Gag-producing strain. Meanwhile, the immunization withL. acidophilusdisplaying both Gag and FliC resulted in an increase of Gag-specific IgA-secreting cells. These results suggested that the Gag-displayingL. acidophiluselicited specific immune responses and the coexistence of FliC conferred an adjuvant effect on local IgA production.

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Section: Discussionmentioning

confidence: 99%

Construction and Immunological Evaluation of Dual Cell Surface Display of HIV-1 Gag and Salmonella enterica Serovar Typhimurium FliC in Lactobacillus acidophilus for Vaccine Delivery

Kajikawa

Zhang

Long

et al. 2012

Clin Vaccine Immunol

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“…IM administration of influenza virus epitope peptides carried by recombinant flagellin (originated from nonvirulent salmonella bacteria) induced both humoral and cellular responses and conferred some protection against lethal challenge (H5N1) [410]. The intensive response to flagellin is mediated by toll-like receptor 5, linking innate and adaptive immunity11.…”

Section: Flagellinmentioning

confidence: 99%

Selection of Adjuvants for Enhanced Vaccine Potency

Wang¹,

Singh²

2011

WJV

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“…[155][156][157][158][159][160] Vaccines based solely on conserved internal antigens must rely on cell-mediated immunity, because the target antigens are not exposed on the surface of an influenza virion for antibody binding. 161 In rodent models, such T cell-based vaccines can protect from challenge.…”

Section: Universal Influenza Vaccine Approachesmentioning

confidence: 99%