A universal design for a DNA probe providing ratiometric fluorescence detection by generation of silver nanoclusters

Abstract

and singe-nucleotide specificity, expanding the breadth of applicability of these cost-effective probes for biomolecular detection.

Metal Nanoclusters–Based Ratiometric Fluorescent Probes from Design to Sensing Applications

“…The in situ synthesis can obtain the products directly without elaborate molecular design and complicated synthesis. Thus, it is a facile, effective, and controllable way to prepare ratiometric fluorescent probes . In the typical procedure, the precursors and ligands are added to the system simultaneously or sequentially, with the reaction progresses, the ratio fluorescent probe is finally synthesized in situ.…”

“…Their process is the CDs–AuNCs synthesized via situ microwaving a mixture of gold seeds solution (HClAu 4 ) and carbon precursor (glucose). Fygenson's group prepared dual‐emitting DNA‐stabilized Ag NCs using similar in situ technique, which can be used for ratiometric fluorescence DNA detection . Furthermore, Yu's group synthesized the polyethylenimine‐modified Ru(bpy) 3 2+ ‐doped silica nanoparticles according to the Stöber method, which was used as the template particle to grow Ag NCs in situ to prepare dual‐emission PL nanoprobe .…”

Large-scale investigation of the effects of nucleobase sequence on fluorescence excitation and Stokes shifts of DNA-stabilized silver clusters

“…[2][3][4] Researchers have collectively reported on Ag N -DNAs stabilized by thousands of different DNA strands, representing a diverse palette of fluorescence properties: emission spectra peaked at 450 nm to 1000 nm, 5,6 quantum yields from 3% to 93%, [7][8][9][10][11][12][13] Stokes shifts up to 0.73 eV (5900 cm −1 ), 14 and light-up or color-switching behavior induced by stimuli. [15][16][17][18] These diverse and sequence-tunable fluorescent Ag N -DNAs are promising for applications ranging from sensing [19][20][21][22] and molecular logic schemes 23 to background-free fluorescence microscopy [24][25][26] and nanophotonics. 10,27,28 Recent breakthroughs are rapidly advancing our understanding of the structures of certain Ag N -DNAs, 5,7,14,[29][30][31] , largely due to detailed studies of about 20 different Ag N -DNA species purified by high performance liquid chromatography (HPLC).…”

A Multicolor Chameleon DNA‐templated Silver Nanocluster and Its Application for Ratiometric Fluorescence Target Detection with Exponential Signal Response

“…Petty and co‐workers reported that by hybridizing with complementary strands, the emission of AgNC could convert from violet to blue–green or near‐infrared . Donnell et al constructed a ratiometric AgNC probe showing distinct fluorescence emission before and after binding with target DNA . A chameleon AgNC obtaining orange‐to‐green fluorescence transformation was designed for Hg 2+ detection .…”