A universal CRISPR/Cas12a-mediated AuNPs aggregation-based surface-enhanced Raman scattering (CRISPR/Cas-SERS) platform for virus gene detection

Su, Ailing; Liu, Yuan; Cao, Xiumian; Xu, Weiqing; Liang, Chaozhao; Xu, Shuping

doi:10.1016/j.snb.2022.132295

Cited by 26 publications

(18 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One example involves screening specific crRNAs for targeting the genes of HPV 16 and HPV 18 types in a Cas12a-based sensing system. 41 Fig. 3b-d presents the agarose gel electrophoresis results for validity and specificity tests.…”

Section: Grna Designmentioning

confidence: 99%

“…59 (E) Schematic illustration of the CRISPR/Cas12a-mediated AuNPs polymerization SERS platform for viral gene detection. 41 (F) Schematic diagram of the CRISPR/Cas12abased SERS microfluidic paper assay device (μPAD) for ultrasensitive detection of food pathogenic bacteria. 60 (G) Schematic diagram of a CRISPR/ Cas12a integrated SERS nanoplatform with a chimeric DNA/RNA hairpin guidance for nucleic acid detection.…”

Section: Raman Reporter Modifies Metal Surface: Sers Signal Turn-offmentioning

confidence: 99%

See 1 more Smart Citation

Current advance of CRISPR/Cas-based SERS technology

Wang

Chang

et al. 2023

Sens. Diagn.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Grna Designmentioning

confidence: 99%

Section: Raman Reporter Modifies Metal Surface: Sers Signal Turn-offmentioning

confidence: 99%

Current advance of CRISPR/Cas-based SERS technology

Wang

Chang

et al. 2023

Sens. Diagn.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additionally, instead of being cut by Cas enzymes after anchoring SERS tags or reporter molecules, ssDNA can be cleaved beforehand. [164] Degraded linkers fail to crosslink SERS tags, leaving them in dispersed form and causing weak SERS intensities. This strategy can be combined with a follow-up centrifugation step and changed into a turn-on method by measuring SERS signals in the supernatant.…”

Section: Surface-enhanced Raman Spectroscopymentioning

confidence: 99%

“…This method also demonstrated versatility by enclosing cysteine in liposomes for colorimetric detection. Additionally, instead of being cut by Cas enzymes after anchoring SERS tags or reporter molecules, ssDNA can be cleaved beforehand [164] . Degraded linkers fail to crosslink SERS tags, leaving them in dispersed form and causing weak SERS intensities.…”

Section: Crispr‐based Nucleic Acid Detection Systems Integrated With ...mentioning

confidence: 99%

CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics

et al. 2023

View full text Add to dashboard Cite

Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires a long turnaround time, expensive equipment, and trained personnel, limiting its widespread availability and diagnostic capacity. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR) technology has recently demonstrated capability for nucleic acid detection with high sensitivity and specificity. CRISPR‐mediated biosensing holds great promise for revolutionizing nucleic acid testing procedures and developing point‐of‐care diagnostics. This review focuses on recent developments in both fundamental CRISPR biochemistry and CRISPR‐based nucleic acid detection techniques. Four ongoing research hotspots in molecular diagnostics‐target preamplification‐free detection, microRNA (miRNA) testing, non‐nucleic‐acid detection, and SARS‐CoV‐2 detection‐are also covered.

show abstract

“…Using this SERS-optimizing system improved the sensitivity of the viral DNAs, including HBV, human papillomavirus 16 (HPV-16), and HPV-18, with a 1 aM LOD. Su et al showed a similar strategy to detect HPV DNA using a pair of AuNP, which are connected by ssDNA [ 60 ]. Raman dye-functionalized two AuNPs modified each capture DNA.…”

Section: Sensitive Plasmonic Nanobiosensors For Viral Dna Detectionmentioning

confidence: 99%

Recent Development in Plasmonic Nanobiosensors for Viral DNA/RNA Biomarkers

Park

Choi

2022

Biosensors

View full text Add to dashboard Cite

Recently, due to the coronavirus pandemic, the need for early diagnosis of infectious diseases, including viruses, is emerging. Though early diagnosis is essential to prevent infection and progression to severe illness, there are few technologies that accurately measure low concentrations of biomarkers. Plasmonic nanomaterials are attracting materials that can effectively amplify various signals, including fluorescence, Raman, and other optical and electromagnetic output. In this review, we introduce recently developed plasmonic nanobiosensors for measuring viral DNA/RNA as potential biomarkers of viral diseases. In addition, we discuss the future perspective of plasmonic nanobiosensors for DNA/RNA detection. This review is expected to help the early diagnosis and pathological interpretation of viruses and other diseases.

show abstract

A universal CRISPR/Cas12a-mediated AuNPs aggregation-based surface-enhanced Raman scattering (CRISPR/Cas-SERS) platform for virus gene detection

Cited by 26 publications

References 38 publications

Current advance of CRISPR/Cas-based SERS technology

Current advance of CRISPR/Cas-based SERS technology

CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics

Recent Development in Plasmonic Nanobiosensors for Viral DNA/RNA Biomarkers

Contact Info

Product

Resources

About